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sw480 cell line (ATCC)

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ATCC sw480 cell line

FAM65A promotes CRC cell proliferation and inhibits cell apoptosis (A) Western blot analysis FAM65A expression in human normal colon cells and CRC cells was assessed. (B) Western blot analysis FAM65A knockdown efficiency in <t>SW480</t> and LOVO cells. (C) CCK8 assay was performed with shCtrl and shFAM65A in SW480 and LOVO cell lines, n = 3, ∗∗∗ p < 0.001. (D) The outcomes of the colony formation assay for shCtrl and shFAM65A in SW480 and LOVO cells were reported. (E) A quantitative analysis of the colony formation assay was conducted, n = 6, ∗ p < 0.05, ∗∗ p < 0.01. (F) The results of the EdU assay for shCtrl and shFAM65A in SW480 and LOVO cells were presented, where EdU-positive stained cells exhibited green fluorescence and nuclei displayed blue fluorescence. Scale bars, 100 μm. (G) A quantitative analysis of the EdU assay results was performed, n = 3, ∗∗∗ p < 0.001. (H) The results of the apoptosis assay for shCtrl and shFAM65A in SW480 and LOVO cells were documented. Scale bars, 50 μm. (I) A quantitative analysis of the apoptosis assay was conducted, n = 3, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (J) The western blot results for the expression of Ki-67, cleaved caspase 3, Bcl-2, and Bax in SW480 and LOVO cells for both shCtrl and shFAM65A were presented. Data are presented as mean ± SEM of biologically independent experiments.

Sw480 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sw480 cell line/product/ATCC
Average 99 stars, based on 8371 article reviews

sw480 cell line - by Bioz Stars, 2026-03

99/100 stars

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1) Product Images from "FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling"

Article Title: FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling

Journal: iScience

doi: 10.1016/j.isci.2026.114662

Figure Legend Snippet: FAM65A promotes CRC cell proliferation and inhibits cell apoptosis (A) Western blot analysis FAM65A expression in human normal colon cells and CRC cells was assessed. (B) Western blot analysis FAM65A knockdown efficiency in SW480 and LOVO cells. (C) CCK8 assay was performed with shCtrl and shFAM65A in SW480 and LOVO cell lines, n = 3, ∗∗∗ p < 0.001. (D) The outcomes of the colony formation assay for shCtrl and shFAM65A in SW480 and LOVO cells were reported. (E) A quantitative analysis of the colony formation assay was conducted, n = 6, ∗ p < 0.05, ∗∗ p < 0.01. (F) The results of the EdU assay for shCtrl and shFAM65A in SW480 and LOVO cells were presented, where EdU-positive stained cells exhibited green fluorescence and nuclei displayed blue fluorescence. Scale bars, 100 μm. (G) A quantitative analysis of the EdU assay results was performed, n = 3, ∗∗∗ p < 0.001. (H) The results of the apoptosis assay for shCtrl and shFAM65A in SW480 and LOVO cells were documented. Scale bars, 50 μm. (I) A quantitative analysis of the apoptosis assay was conducted, n = 3, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (J) The western blot results for the expression of Ki-67, cleaved caspase 3, Bcl-2, and Bax in SW480 and LOVO cells for both shCtrl and shFAM65A were presented. Data are presented as mean ± SEM of biologically independent experiments.

Techniques Used: Western Blot, Expressing, Knockdown, CCK-8 Assay, Colony Assay, EdU Assay, Staining, Fluorescence, Apoptosis Assay

FAM65A promotes CRC cell migration in vitro (A) Transwell migration assay was conducted to evaluate the migration capabilities of shCtrl and shFAM65A in SW480 and LOVO cell lines. Scale bars, 50 μm. (B) A quantitative analysis of the Transwell migration assay was performed, n = 3, ∗∗∗ p < 0.001. (C) The results of wound healing assay comparing shCtrl and shFAM65A in SW480 and LOVO cells were obtained. Scale bars, 50 μm. (D) A quantitative analysis of the wound healing assay was also conducted, n = 3, ∗∗∗ p < 0.001. (E) Western blot analysis was performed to assess the expression levels of EMT markers in SW480 and LOVO cells. Data are presented as mean ± SEM of biologically independent experiments.

Figure Legend Snippet: FAM65A promotes CRC cell migration in vitro (A) Transwell migration assay was conducted to evaluate the migration capabilities of shCtrl and shFAM65A in SW480 and LOVO cell lines. Scale bars, 50 μm. (B) A quantitative analysis of the Transwell migration assay was performed, n = 3, ∗∗∗ p < 0.001. (C) The results of wound healing assay comparing shCtrl and shFAM65A in SW480 and LOVO cells were obtained. Scale bars, 50 μm. (D) A quantitative analysis of the wound healing assay was also conducted, n = 3, ∗∗∗ p < 0.001. (E) Western blot analysis was performed to assess the expression levels of EMT markers in SW480 and LOVO cells. Data are presented as mean ± SEM of biologically independent experiments.

Techniques Used: Migration, In Vitro, Transwell Migration Assay, Wound Healing Assay, Western Blot, Expressing

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Journal: iScience

Article Title: FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling

doi: 10.1016/j.isci.2026.114662

Figure Lengend Snippet: FAM65A promotes CRC cell proliferation and inhibits cell apoptosis (A) Western blot analysis FAM65A expression in human normal colon cells and CRC cells was assessed. (B) Western blot analysis FAM65A knockdown efficiency in SW480 and LOVO cells. (C) CCK8 assay was performed with shCtrl and shFAM65A in SW480 and LOVO cell lines, n = 3, ∗∗∗ p < 0.001. (D) The outcomes of the colony formation assay for shCtrl and shFAM65A in SW480 and LOVO cells were reported. (E) A quantitative analysis of the colony formation assay was conducted, n = 6, ∗ p < 0.05, ∗∗ p < 0.01. (F) The results of the EdU assay for shCtrl and shFAM65A in SW480 and LOVO cells were presented, where EdU-positive stained cells exhibited green fluorescence and nuclei displayed blue fluorescence. Scale bars, 100 μm. (G) A quantitative analysis of the EdU assay results was performed, n = 3, ∗∗∗ p < 0.001. (H) The results of the apoptosis assay for shCtrl and shFAM65A in SW480 and LOVO cells were documented. Scale bars, 50 μm. (I) A quantitative analysis of the apoptosis assay was conducted, n = 3, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (J) The western blot results for the expression of Ki-67, cleaved caspase 3, Bcl-2, and Bax in SW480 and LOVO cells for both shCtrl and shFAM65A were presented. Data are presented as mean ± SEM of biologically independent experiments.

Article Snippet: SW480 cell line , ATCC , CCL-228.

Techniques: Western Blot, Expressing, Knockdown, CCK-8 Assay, Colony Assay, EdU Assay, Staining, Fluorescence, Apoptosis Assay

Journal: iScience

Article Title: FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling

doi: 10.1016/j.isci.2026.114662

Figure Lengend Snippet: FAM65A promotes CRC cell migration in vitro (A) Transwell migration assay was conducted to evaluate the migration capabilities of shCtrl and shFAM65A in SW480 and LOVO cell lines. Scale bars, 50 μm. (B) A quantitative analysis of the Transwell migration assay was performed, n = 3, ∗∗∗ p < 0.001. (C) The results of wound healing assay comparing shCtrl and shFAM65A in SW480 and LOVO cells were obtained. Scale bars, 50 μm. (D) A quantitative analysis of the wound healing assay was also conducted, n = 3, ∗∗∗ p < 0.001. (E) Western blot analysis was performed to assess the expression levels of EMT markers in SW480 and LOVO cells. Data are presented as mean ± SEM of biologically independent experiments.

Article Snippet: SW480 cell line , ATCC , CCL-228.

Techniques: Migration, In Vitro, Transwell Migration Assay, Wound Healing Assay, Western Blot, Expressing

Knockdown of ARG2 inhibits the proliferative ability of CRC cells. (A) ARG2 protein expression in CRC cell lines and normal intestinal epithelial cells was determined by Western blot. Western blot was also used to detect the knockdown efficiency of ARG2 protein in HCT116 (B) and SW480 (C) cells. (D, E) CCK‐8 assay analysis of proliferation in ARG2‐knockdown (shARG2) and NC HCT116/SW480 cells. “Relative cell number” in (D) and (E) is derived from CCK‐8 assay results, representing the ratio of absorbance at each time point to Day 0 (baseline). It reflects the relative change in viable cell quantity over the culture period. (F–H) Colony formation assay to evaluate proliferation of shARG2 and NC HCT116/SW480 cells. Data in (D, E) and (F–H) are presented as mean ± SD of three independent experiments. Statistical significance was determined by two‐way ANOVA (D, E) and one‐way ANOVA (G, H). ns, no significant difference. *** p < 0.001, **** p < 0.0001.

Journal: Cancer Medicine

Article Title: Arginase 2 Promotes Colorectal Cancer Metastasis via PI3K/AKT Pathway Activation and Regulates Tumor Immune Infiltration

doi: 10.1002/cam4.71567

Figure Lengend Snippet: Knockdown of ARG2 inhibits the proliferative ability of CRC cells. (A) ARG2 protein expression in CRC cell lines and normal intestinal epithelial cells was determined by Western blot. Western blot was also used to detect the knockdown efficiency of ARG2 protein in HCT116 (B) and SW480 (C) cells. (D, E) CCK‐8 assay analysis of proliferation in ARG2‐knockdown (shARG2) and NC HCT116/SW480 cells. “Relative cell number” in (D) and (E) is derived from CCK‐8 assay results, representing the ratio of absorbance at each time point to Day 0 (baseline). It reflects the relative change in viable cell quantity over the culture period. (F–H) Colony formation assay to evaluate proliferation of shARG2 and NC HCT116/SW480 cells. Data in (D, E) and (F–H) are presented as mean ± SD of three independent experiments. Statistical significance was determined by two‐way ANOVA (D, E) and one‐way ANOVA (G, H). ns, no significant difference. *** p < 0.001, **** p < 0.0001.

Article Snippet: Human CRC cell lines (DLD1, LoVo, HCT116, and SW480) and the normal colon epithelial cell line (NCM460) were purchased from the ATCC.

Techniques: Knockdown, Expressing, Western Blot, CCK-8 Assay, Derivative Assay, Colony Assay

Knockdown of ARG2 suppresses invasion and migration capabilities of CRC cells in vitro and restrains EMT progression. (A–D) Wound‐healing assay was performed to evaluate the migration capacity of shARG2 and NC HCT116/SW480 cells. The wound closure after 24 h post scratching was measured. Scale bar represents 200 μm. (E–G) Transwell assay was used to assess the invasion capacity of shARG2 and NC HCT116/SW480 cells. Scale bar represents 50 μm. (H, I) The protein expressions of EMT markers in shARG2 and NC HCT116/SW480 cells, including ZEB1, N‐cadherin, and MMP2, were measured by western blotting. Data in (A–G) are presented as mean ± SD of three independent experiments. Statistical significance was determined by one‐way ANOVA (B, D, F, G). ns, no significant difference. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Cancer Medicine

Article Title: Arginase 2 Promotes Colorectal Cancer Metastasis via PI3K/AKT Pathway Activation and Regulates Tumor Immune Infiltration

doi: 10.1002/cam4.71567

Figure Lengend Snippet: Knockdown of ARG2 suppresses invasion and migration capabilities of CRC cells in vitro and restrains EMT progression. (A–D) Wound‐healing assay was performed to evaluate the migration capacity of shARG2 and NC HCT116/SW480 cells. The wound closure after 24 h post scratching was measured. Scale bar represents 200 μm. (E–G) Transwell assay was used to assess the invasion capacity of shARG2 and NC HCT116/SW480 cells. Scale bar represents 50 μm. (H, I) The protein expressions of EMT markers in shARG2 and NC HCT116/SW480 cells, including ZEB1, N‐cadherin, and MMP2, were measured by western blotting. Data in (A–G) are presented as mean ± SD of three independent experiments. Statistical significance was determined by one‐way ANOVA (B, D, F, G). ns, no significant difference. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human CRC cell lines (DLD1, LoVo, HCT116, and SW480) and the normal colon epithelial cell line (NCM460) were purchased from the ATCC.

Techniques: Knockdown, Migration, In Vitro, Wound Healing Assay, Transwell Assay, Western Blot

GO and KEGG enrichment analyses revealed that ARG2 is involved in the regulation of the PI3K/AKT signaling pathway in CRC. (A) The GO bubble plot illustrates the top enriched Gene Ontology terms across biological processes (BP), cellular components (CC), and molecular functions (MF). (B) The KEGG analysis highlights significantly enriched pathways, among which the PI3K/AKT signaling pathway was involved. (C) GSEA analysis revealed a significant association between ARG2 expression and the PI3K/AKT signaling pathway. (D, E) Protein levels of total AKT, p‐AKT, p‐PI3K, mTOR, and p‐mTOR were measured by western blotting in shARG2 and NC HCT116/SW480 cells. Statistical significance for GSEA was determined by the permutation test, with FDR < 0.05 considered significant.

Journal: Cancer Medicine

Article Title: Arginase 2 Promotes Colorectal Cancer Metastasis via PI3K/AKT Pathway Activation and Regulates Tumor Immune Infiltration

doi: 10.1002/cam4.71567

Figure Lengend Snippet: GO and KEGG enrichment analyses revealed that ARG2 is involved in the regulation of the PI3K/AKT signaling pathway in CRC. (A) The GO bubble plot illustrates the top enriched Gene Ontology terms across biological processes (BP), cellular components (CC), and molecular functions (MF). (B) The KEGG analysis highlights significantly enriched pathways, among which the PI3K/AKT signaling pathway was involved. (C) GSEA analysis revealed a significant association between ARG2 expression and the PI3K/AKT signaling pathway. (D, E) Protein levels of total AKT, p‐AKT, p‐PI3K, mTOR, and p‐mTOR were measured by western blotting in shARG2 and NC HCT116/SW480 cells. Statistical significance for GSEA was determined by the permutation test, with FDR < 0.05 considered significant.

Article Snippet: Human CRC cell lines (DLD1, LoVo, HCT116, and SW480) and the normal colon epithelial cell line (NCM460) were purchased from the ATCC.

Techniques: Expressing, Western Blot

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1) Product Images from "FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling"

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