crl  (ATCC)

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: IL-4-Induced M2 macrophage polarization promotes the progression of LPS. RAW264.7 cells were divided into two groups: control group, IL-4 group. (A) Flow cytometry was used to detect the expression level of CD206 on RAW264.7 cells in each group. (B) ELISA was performed to measure the secretion level of IL-10 in RAW264.7 cells from each group. (C) Western Blot was employed to determine the protein expression level of Arg-1 in RAW264.7 cells of each group. A Transwell co-culture system was utilized, which included two groups: control co-culture group (LPS cells co-cultured with untreated RAW264.7 cells). IL-4 co-culture group (LPS cells co-cultured with RAW264.7 cells pre-treated with IL-4). (D) EdU incorporation assay was used to detect the proliferative activity of SW872 cells and 94T778 cells in each group. (E-F) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 cells and 94T778 cells in each group. Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Culture Assay, Cell Culture, Activity Assay, Migration

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: mTORC2 regulates TAM via PPAR-γ/CD36 pathway to promote LPS progression. LPS cells (SW872 and 94T778) were co-cultured with RAW264.7 macrophages, and the groups were divided as follows: control co-culture group, IL-4 co-culture group, IL-4+JR-AB2-011 co-culture group, IL-4+JR-AB2-011+LPA co-culture group. (A) EdU assay was used to detect the proliferation of SW872 and 94T778 cells in each co-culture group. (B-C) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 and 94T778 cells in each co-culture group. Data are presented as the mean±SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Cell Culture, Control, Co-Culture Assay, EdU Assay, Migration