ccl 185 sw872 atcc cat  (ATCC)

Journal: Neoplasia (New York, N.Y.)

Article Title: OTUD4 regulates pancreatic cancer progression via Hippo/YAP axis

doi: 10.1016/j.neo.2026.101285

Figure Lengend Snippet: OTUD4 is crucial for the progression of PAAD. A . Transfection of siControl or siOTUD4 #1/#2 was performed in PAAD cells. The knockdown efficiency of OTUD4 at the protein level was confirmed by Western blot analysis. B-C. The relative mRNA expression level of OTUD4 was measured by RT-qPCR following transfection with siControl or siOTUD4 #1/#2. D-E. Proliferation rate of PAAD cells transfected with siControl or siOTUD4 #1/#2 was measured using the CCK-8 assay. F-I. The migration and invasion abilities of PAAD cells following transfection with siControl or siOTUD4 #1/#2 was assessed by Transwell assay. J-M. Proliferation rate of PAAD cells transfected with siControl or siOTUD4 #1/#2 was assessed using the EdU assay. Hoechst 33342 was used for nuclear staining, and the scale bar is 200 μm. N-Q. Apoptosis of PAAD cells transfected with siControl or siOTUD4 was measured by flow cytometry following staining with FITC and PI. R-V. SW1990 cells transfected with shControl and shOTUD4 were injected subcutaneously into nude mice. The nude mice were sacrificed after 35 days of injection, and the tumor weight ( S ) and volume ( T ) were measured. IHC assays were utilized for evaluating the Ki-67 positive rate ( U-V ). n = 6. The scale bar is 400 μm for 10X and 100 μm for 40X. All the experiments were performed in triplicate. All data are presented as mean ± SDs. Statistical methods: Student’s t -test for B-C, G, I, K, M, O, Q, S and V; two-way ANOVA for D and E. ** P < 0.01; *** P < 0.001.

Article Snippet: The AsPC-1, SW1990, HEK293T cell lines were all originated from American Type Culture Collection (ATCC).

Techniques: Transfection, Knockdown, Western Blot, Expressing, Quantitative RT-PCR, CCK-8 Assay, Migration, Transwell Assay, EdU Assay, Staining, Flow Cytometry, Injection

Journal: Neoplasia (New York, N.Y.)

Article Title: OTUD4 regulates pancreatic cancer progression via Hippo/YAP axis

doi: 10.1016/j.neo.2026.101285

Figure Lengend Snippet: The hydrolytic activity of OTUD4 is essential in regulating YAP function. A . A schematic diagram depicts the C45A mutation of OTUD4. B-C. The protein level of YAP after transfecting either siControl or siOTUD4 #1/#2 in AsPC-1 cells ( B ) and SW1990 cells ( C ) was measured through western-blot. D. RT-qPCR assays were used for evaluating the mRNA expression levels of CTGF and CYR61 in PAAD cells after transfection with siControl or siOTUD4. E-F. Dual-luciferase reporter gene assays were conducted in siControl-transfected or siOTUD4-transfected AsPC-1 (E) and SW1990 cells (F) to evaluate TEAD4 transcriptional activity. G-H. Immunoblotting assays were performed to observe the protein level of YAP after transfecting Flag or Flag-OTUD4 WT or Flag-OTUD4 C45A in AsPC-1 cells ( G ) and SW1990 cells (H) . I-J. Quantification of CTGF and CYR61 expression levels by RT-qPCR was performed in PAAD cells transfected with Flag or Flag-OTUD4 WT or Flag-OTUD4 C45A . K-L. TEAD4 transcriptional activity were measured using a dual-luciferase reporter assay after cells transfection with Flag, Flag-OTUD4 WT , or Flag-OTUD4 C45A plasmids. All the experiments were performed in triplicate. All the data are presented as the means ± SDs. Statistical methods: Student’s t -test for D, E-F, I and J-K. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The AsPC-1, SW1990, HEK293T cell lines were all originated from American Type Culture Collection (ATCC).

Techniques: Activity Assay, Mutagenesis, Western Blot, Quantitative RT-PCR, Expressing, Transfection, Luciferase, Reporter Assay

Journal: Neoplasia (New York, N.Y.)

Article Title: OTUD4 regulates pancreatic cancer progression via Hippo/YAP axis

doi: 10.1016/j.neo.2026.101285

Figure Lengend Snippet: YAP overexpression rescues OTUD4 knockdown-induced suppression of PAAD. A. SW1990 cells were transfected with siControl, siOTUD4 , or siOTUD4+Myc-YAP, respectively. The protein levels of OTUD4 and YAP were measured by immunoblotting. B. To determine the effect of OTUD4 and YAP, the mRNA levels of CTGF and CYR61 in SW1990 cells were measured using RT-qPCR after the cells were transfected with siRNAs and Myc-YAP plasmids. C. CCK-8 assay was conducted to evaluate cell proliferation of SW1990 cells after transfection with siControl, siOTUD4+Myc, or siOTUD4+Myc-YAP. D-E. To assess migration and invasion, transwell assays were performed using SW1990 cells transfected with the specified siRNA and plasmid. F-G. The EdU assay was used to further evaluate the proliferative capacity of SW1990 cells following transfection with indicated siRNA and plasmid. The nuclei were stained with Hoechst 33342.The scale bar is 200 μm. H-I. Flow cytometry was performed to analyze apoptosis in SW1990 cells transfected with specific siRNAs and plasmids. J-N. SW1990 cells transfected with shControl, shOTUD4, or shOTUD4 + Myc-YAP were subcutaneously injected into nude mice. After 35 days, the mice were euthanized ( J ), and tumor weight ( K ) and volume ( L ) were measured. In addition, IHC staining was performed to assess the expression of Ki-67 ( M ), and the percentage of Ki-67-positive cells was quantified ( N ). n = 6. All the experiments were performed in triplicate. All the data are presented as the means ± SDs. Statistical methods: Student’s t -test for B, E, J, I, K and N . Two-way ANOVA for C and L. ** P < 0.01 *** P < 0.001.

Article Snippet: The AsPC-1, SW1990, HEK293T cell lines were all originated from American Type Culture Collection (ATCC).

Techniques: Over Expression, Knockdown, Transfection, Western Blot, Quantitative RT-PCR, CCK-8 Assay, Migration, Plasmid Preparation, EdU Assay, Staining, Flow Cytometry, Injection, Immunohistochemistry, Expressing

Journal: Neoplasia (New York, N.Y.)

Article Title: OTUD4 regulates pancreatic cancer progression via Hippo/YAP axis

doi: 10.1016/j.neo.2026.101285

Figure Lengend Snippet: OTUD4 interacts with YAP to promote its stability. A. Immunofluorescence (IF) analysis was performed to determine the subcellular localization of OTUD4 and YAP. OTUD4 is shown in green, and YAP is displayed in red. Nuclei were stained with DAPI. The scale bar represents 50 μm. B. The endogenous interaction between OTUD4 and YAP was investigated by co-IP. Immunoprecipitation was performed using anti-OTUD4 or anti-YAP on lysates of SW1990 cells, and the associated proteins were detected by Western blotting. C. A schematic diagram of the molecular docking structure, which reveals the interaction between the OTU domain of OTUD4 and the WW domain of YAP, was visualized using PyMOL. D-F. Schematic representations of the individual domains of OTUD4 and YAP are provided ( D ). Plasmids encoding full-length OTUD4, YAP, and their respective truncated domains (OTUD4 residues: 1-155, Δ34-155 and 156-1114; YAP residues: 1-171, 1-292, 171-504 and 292-504) were transfected into HEK-293T cells. Co-IP assays show the WW domain of YAP ( E ) and the OTU domain of OTUD4 ( F ) are responsible for their interaction. G, J. MG132 treatment abolished the decrease in YAP expression induced by siOTUD4 in both AsPC-1 ( G ) and SW1990 ( J ) cells. After transfection with siControl or siOTUD4, the cells were treated with 10 μM MG132 for 12 hours. H-I, K-N. The effect of OTUD4 knockdown or overexpression on YAP stability was assessed using cycloheximide (CHX) assays. OTUD4 knockdown reduced YAP stability ( H-I, K-L ), whereas OTUD4 overexpression enhanced it. In contrast, the C45A mutant of OTUD4 failed to promote YAP stabilization ( M-N ). A concentration of 100 μM CHX was used in all experiments. All the experiments were performed in triplicate. All the data are presented as the means ± SDs. Statistical methods: two-way ANOVA for I, L and N . * P < 0.05; *** P < 0.001.

Article Snippet: The AsPC-1, SW1990, HEK293T cell lines were all originated from American Type Culture Collection (ATCC).

Techniques: Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, Transfection, Expressing, Knockdown, Over Expression, Mutagenesis, Concentration Assay

Journal: Neoplasia (New York, N.Y.)

Article Title: OTUD4 regulates pancreatic cancer progression via Hippo/YAP axis

doi: 10.1016/j.neo.2026.101285

Figure Lengend Snippet: OTUD4 stabilizes YAP via decreasing its K48-linked polyubiquitination. A-B. Knockdown of OTUD4 specifically enhanced K48-linked polyubiquitination of YAP without altering K63-linked polyubiquitination. To assess ubiquitination patterns, SW1990 cells were transfected with plasmids encoding HA-tagged wild-type ubiquitin or K48 or K63 ubiquitin ( A ) or K48R mutant ubiquitin or K63R mutant ubiquitin ( B ). Following transfection, cells were treated with 20 μM MG132 for 8 hours before harvesting. YAP was immunoprecipitated using an anti-YAP antibody, and its ubiquitination levels were detected by immunoblotting assay with an anti-HA antibody. C-D. Overexpression of OTUD4 reduced K48-linked polyubiquitination of YAP without affecting K63-linked polyubiquitination. SW1990 cells were co-transfected with plasmids encoding Flag-OTUD4 WT and Myc-YAP, along with HA-tagged wild-type ubiquitin or K48 or K63 ubiquitin ( C ) or K48R mutant ubiquitin or K63R mutant ubiquitin ( D ). After transfection, the cells were treated with 20 μM MG132 for 8 hours, harvested, and subjected to immunoprecipitation using an anti-YAP antibody. Ubiquitination levels of YAP were then examined by Western blot with an anti-HA antibody. E-F. Overexpression of OTUD4 decreased K48-linked polyubiquitination of YAP without altering K63-linked polyubiquitination; however, overexpression of the C45A mutant of OTUD4 did not affect either K48-linked or K63-linked polyubiquitination. SW1990 cells were co-transfected with plasmids encoding either Flag-OTUD4 WT or Flag-OTUD4 C45A and Myc-YAP, together with HA-tagged ubiquitin or K48 ubiquitin or K63 ubiquitin ( E ) or K48R mutant ubiquitin or K63R mutant ubiquitin ( F ). After transfection, cells were treated with 20 μM MG132 for 8 hours, harvested, and subjected to immunoprecipitation using an anti-YAP antibody. YAP ubiquitination levels were then analyzed by Western blot with an anti-HA antibody.

Article Snippet: The AsPC-1, SW1990, HEK293T cell lines were all originated from American Type Culture Collection (ATCC).

Techniques: Knockdown, Ubiquitin Proteomics, Transfection, Mutagenesis, Immunoprecipitation, Western Blot, Over Expression

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: SW780 bladder cancer cells produce floating microbladder-like vesicles (luminal cavities characteristic of urothelial differentiation) in monolayer culture. A. Phase-contrast microscopy of T24 and SW780 bladder cancer cells. B. Scanning electron microscopy (SEM) images showing cell surface morphology. Red arrows indicate floating cyst-like vesicles (microbladder-like, luminal cavities characteristic of urothelial differentiation) produced by the SW780 cell line. Images are representative of the overall cell population.

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Microscopy, Electron Microscopy, Produced

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Encapsulation of pheophorbide a enhances PDT efficacy in 3D tumor spheroids. A. Scanning electron microscopy (SEM) images showing the surface morphology of T24 and SW780 spheroids. B. General appearance of bladder tumor spheroids before treatment. Red arrows indicate detached cyst-like vesicles, i.e. “microbladders” (luminal cavities characteristic of urothelial differentiation); dotted circles highlight internal cyst-like structures embedded within the spheroids. C. Counterstained semi-thin transverse sections (500 nm) of spheroids revealing internal cyst-like structures (dotted circles). D. Two-photon microscopy imaging of pheophorbide a (Pheo) (1 µM) penetration in T24 spheroids after 30 min incubation at 37 °C either in its free or encapsulated formulation. Poly (ethylene oxide)-block-poly (ε-caprolactone) (PEO 5000 -PCL 4000 ) empty micelles; Pheophorbide encapsulated in PEO-PCL micelles (Pheo-PEOPCL). Cyan: nuclei (Hoechst); red: pheophorbide fluorescence. E. Spheroid viability assessed by intracellular ATP quantification at 3- and 6-days post-PDT ([Pheo] = 3 µM). Results include data from 1 to 6 independent experiments (N), with a cumulative number of biological replicates (n) ranging from 6 to 39.

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Encapsulation, Electron Microscopy, Microscopy, Imaging, Incubation, Formulation, Blocking Assay, Fluorescence

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Encapsulation of pheophorbide a in PEO-PCL micelles enhances PDT-induced cytotoxicity in 2D high-grade (T24) and low-grade (SW780) bladder cancer cell cultures. A. Cells were treated with empty poly (ethylene oxide)-block-poly (ε-caprolactone) PEO-PCL micelles (PEOPCL) at polymer concentrations equivalent to those used in pheo-loaded micelles, based on the 1:30 drug-to-polymer weight ratio. B. Cells were exposed to increasing concentrations of free pheophorbide a (Pheo) (nM). C. Cells were treated with increasing concentrations of Pheo encapsulated in PEO-PCL micelles (pheo–PEOPCL) (nM). D. Half-maximal inhibitory concentration (IC₅₀) determination for the Pheo–PEOPCL condition. Cell confluence was monitored by videomicroscopy for 72 h post-PDT ( N > 1; n = 6).

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Encapsulation, Blocking Assay, Polymer, Concentration Assay

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Reduced wound closure in bladder cancer cell monolayers following photodynamic treatment with encapsulated pheophorbide a . A. Kinetics of wound closure over 24 h post-PDT in T24 (grade 3) and SW780 (grade 1) monolayers, monitored by videomicroscopy. Cells were treated with increasing concentrations of pheophorbide a encapsulated in poly (ethylene oxide)-block-poly (ε-caprolactone) PEO-PCL micelles (Pheo-PEO-PCL) (nM). B. Quantification of wound closure ( %) at 24 h post-PDT across the different Pheo-PEO-PCL concentrations. Relative wound density ( %) reflects the proportion of the scratch area repopulated by migrating cells. Data are expressed as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test versus control (0 nM).

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Blocking Assay, Control

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Proof of concept of photodynamic therapy efficacy in a complex 3D engineered bladder tumor model. A. Histological cross-sections of the vesical reconstructed tissues stained with Masson’s trichrome, 72 hours after PDT with 3 µM of either free pheophorbide a (Pheo) or pheophorbide a encapsulated in poly (ethylene oxide)-block-poly (ε-caprolactone) PEO-PCL micelles (Pheo–PEO-PCL). Cells appear in red, and stromal collagens in blue. Tumor cells are outlined with black dotted lines. Representative images are shown. B. Tissue viability assessed using the PrestoBlue assay at 48- and 72-hours post-PDT on engineered bladder substitutes implanted with T24 or SW780 spheroids. Results are expressed as a percentage of the untreated control at each timepoint ± SEM.

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Staining, Blocking Assay, Prestoblue Assay, Control

Journal: Bioresources and Bioprocessing

Article Title: Ulvan and Ulva oligosaccharides from Ulva sp. attenuate osteoarthritis in a high-fat diet and ligamentous meniscal injury-induced rat model

doi: 10.1186/s40643-026-01012-9

Figure Lengend Snippet: Effects of UE and UH on the production of IL-6, MMP-3, and Collagen 2α1 in MIA-induced SW1353 cells. Data are presented as mean ± SD ( n = 3). # indicates significant difference at p < 0.05; ## indicates significant difference at p < 0.01. Collagen 2α1, Collagen Type II Alpha 1; IL, interleukin; MIA, monosodium iodoacetate; MMP, matrix metalloproteinase; UE, Ulva extract; UH, Ulva hydrolysate

Article Snippet: The human chondrosarcoma cell line SW1353 was purchased from the Bioresource Collection and Research Center of the Food Industry Research and Development Institute and the American Type Culture Collection (ATCC).

Techniques: