Journal: Lab on a chip

Article Title: A microfluidic array with cellular valving for single cell co-culture.

doi: 10.1039/c0lc00172d

Figure Lengend Snippet: Fig. 6 Heterotypic single cell co-culture. Unlabelled single SW480 cells co-cultured with single SW480 cells metabolically labelled with the fluorescent product of calcein AM (A). A single cell couple (B), and the same couple immunostained with connexin 43 (red, C). A trap : cell diameter ratio of $2.0 was required for efficient (70%) cell coupling (D).

Article Snippet: Human SW480 epithelial, HT29 colon carcinoma and MCF-7 epithelial-like breast cancer cells were purchased from DSMZ (Germany).

Techniques: Co-Culture Assay, Cell Culture, Metabolic Labelling

Journal: Molecular Vision

Article Title: Protein tyrosine phosphatase-1B (PTP1B) helps regulate EGF-induced stimulation of S-phase entry in human corneal endothelial cells

doi:

Figure Lengend Snippet: Western blot detection of PTP1B protein bands in HCEC. Confluent Passage-2 HCEC were cultured from a newborn (lane 1) and a 74-year-old donor (lane 2) and processed for western blot detection of PTP1B. A commercially prepared whole cell lysate of SW480 cells was used as a positive control (lane 3). Blots showed that the two HCEC samples yielded a band at approximately 50 kDa, indicating the presence of full-length PTP1B, as well as two additional bands of approximately 48 kDa and 46 kDa, possibly representing truncated forms. The same three bands were observed in the SW480 positive control sample, as well as lower molecular weight bands around 39–37 kDa that may result from non-specific proteolytic cleavage. Note the variability in density of the three PTP1B-positive bands within the two HCEC samples.

Article Snippet: A commercially prepared whole cell lysate from SW480 cells, a human colonic adenocarcinoma cell line (Santa Cruz Biotechnology Inc., Santa Cruz, CA), acted as a positive control for PTP1B.

Techniques: Western Blot, Cell Culture, Positive Control, Molecular Weight

Journal: Molecular Vision

Article Title: Protein tyrosine phosphatase-1B (PTP1B) helps regulate EGF-induced stimulation of S-phase entry in human corneal endothelial cells

doi:

Figure Lengend Snippet: Comparison of EGFR and PTP1B protein expression in HCEC cultured from 4 young and 4 older donors. A : Western blots demonstrate the relative expression of EGFR and PTP1B in each of the 8 donor samples. EGFR was expressed in all 8 samples, although the band density varied somewhat among the samples. Three PTP1B bands were observed in most samples. These bands are indicated within brackets. Blots for the positive controls for EGFR (EGF-treated A431cells) and PTP1B (SW480 cells) are not shown, but bands were obtained at the same relative molecular weights as in the HCEC samples. β-Actin was used as a loading control for both EGFR and PTP1B. B : Comparison of the average band density for EGFR and PTP1B in samples from young and older donors. Bars represent SEM. Asterisk indicates statistical significance (p=0.024).

Article Snippet: A commercially prepared whole cell lysate from SW480 cells, a human colonic adenocarcinoma cell line (Santa Cruz Biotechnology Inc., Santa Cruz, CA), acted as a positive control for PTP1B.

Techniques: Comparison, Expressing, Cell Culture, Western Blot, Control

Journal: Biomedicines

Article Title: Metformin Enhancement of Therapeutic Effects of 5-Fluorouracil and Oxaliplatin in Colon Cancer Cells and Nude Mice

doi: 10.3390/biomedicines10050955

Figure Lengend Snippet: Combination therapy led to cell arrest in different phases in HT29, SW480, and SW620. CRC cells were analyzed using flow cytometry after 24 h of treatment with metformin (5 mM), FuOx (2 µM), and the combination of metformin and FuOx. Representative flow cytometry results ( left panel) and histograms ( right panel) reveal cell cycle distribution and quantitative analysis, respectively, in ( A ) HT29, ( B ) SW480, and ( C ) SW620 cell lines. Data are presented as mean ± SEM. Cont, control; Met, metformin; FuOx, 5FU and oxaliplatin; Comb, combination of metformin and FuOx.

Article Snippet: An HT29 colon cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA), and SW480 and SW620 colon cancer cell lines were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Flow Cytometry, Control

Journal: Biomedicines

Article Title: Metformin Enhancement of Therapeutic Effects of 5-Fluorouracil and Oxaliplatin in Colon Cancer Cells and Nude Mice

doi: 10.3390/biomedicines10050955

Figure Lengend Snippet: The combination therapy group induced greater apoptosis than metformin alone and FuOx alone groups in HT29, SW480, and SW620 cell lines. CRC cells were analyzed using flow cytometry after 72 h of treatment with metformin (5 mM), FuOx (2 µM), and the combination of metformin and FuOx. Representative flow cytometry dot plots ( left panel) and histograms ( right panel) reveal cell apoptosis and quantitative analysis, respectively, for ( A ) HT29, ( B ) SW480, and ( C ) SW620 cell lines. Data are presented as mean ± SEM. Cont, control; Met, metformin; FuOx, 5FU and oxaliplatin; Comb, combination of metformin and FuOx.

Article Snippet: An HT29 colon cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA), and SW480 and SW620 colon cancer cell lines were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Flow Cytometry, Control

Journal: Biomedicines

Article Title: Metformin Enhancement of Therapeutic Effects of 5-Fluorouracil and Oxaliplatin in Colon Cancer Cells and Nude Mice

doi: 10.3390/biomedicines10050955

Figure Lengend Snippet: Metformin enhanced the expression of FuOx by modulating Mcl-1 and Bim in HT29, SW480, and SW620 cell lines. Mcl-1 and Bim protein levels in ( A ) HT29, ( B ) SW480, and ( C ) SW620 cell lines were analyzed through Western blot after 72 h treatment with metformin (5 mM), FuOx (2 µM), and the combination of metformin and FuOx. Data are presented as mean ± SEM. Cont, control; Met, metformin; FuOx, 5FU and oxaliplatin; Comb, combination of metformin and FuOx.

Article Snippet: An HT29 colon cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA), and SW480 and SW620 colon cancer cell lines were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Expressing, Western Blot, Control