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lanc  (MedChemExpress)


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    MedChemExpress lanc
    Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Lanc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 2635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lanc/product/MedChemExpress
    Average 99 stars, based on 2635 article reviews
    lanc - by Bioz Stars, 2026-02
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    1) Product Images from "Network pharmacology analysis of Lanatoside C: molecular targets and mechanisms in the treatment of ulcerative colitis"

    Article Title: Network pharmacology analysis of Lanatoside C: molecular targets and mechanisms in the treatment of ulcerative colitis

    Journal: Frontiers in Molecular Biosciences

    doi: 10.3389/fmolb.2025.1552360

    Anti-inflammatory effects of LanC in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected by CCK-8 assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Anti-inflammatory effects of LanC in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected by CCK-8 assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction



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    Anti-inflammatory effects of LanC in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected by CCK-8 assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Network pharmacology analysis of Lanatoside C: molecular targets and mechanisms in the treatment of ulcerative colitis

    doi: 10.3389/fmolb.2025.1552360

    Figure Lengend Snippet: Anti-inflammatory effects of LanC in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected by CCK-8 assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Following 24 h incubation, cells were treated with indicated concentrations of LanC (MedChemExpress) for 24 h. CCK-8 solution (MedChemExpress) was added to the wells and the plates were returned to the 37°C humidified chamber under a 5% CO 2 atmosphere for 1 h. The absorbance (A) values of cells were assessed at 450 nm using an enzyme marker, and the cell viability was computed.

    Techniques: CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction