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lanc  (MedChemExpress)
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Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
Lanc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ultrasonic lance up200st  (Hielscher Ultrasonics)
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Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
Ultrasonic Lance Up200st, supplied by Hielscher Ultrasonics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lance-williams dissimilarity algorithm  (RStudio)
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Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
Lance Williams Dissimilarity Algorithm, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lance bur 859 lf.fg.014  (Komet GmbH)
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Komet GmbH lance bur 859 lf.fg.014
Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
Lance Bur 859 Lf.Fg.014, supplied by Komet GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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20-gauge microvitreoretinal knife v-lance knife  (alcon inc)
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Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
20 Gauge Microvitreoretinal Knife V Lance Knife, supplied by alcon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v-lance micro-scalpel  (Alcon Japan Ltd)
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Alcon Japan Ltd v-lance micro-scalpel
Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
V Lance Micro Scalpel, supplied by Alcon Japan Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lance-chopper sp-9989  (ASICO LLC)
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ASICO LLC lance-chopper sp-9989
Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
Lance Chopper Sp 9989, supplied by ASICO LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sonopuls equipped with bandelin uw2070 and ms73 lance  (Bandelin Electronic)
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Bandelin Electronic sonopuls equipped with bandelin uw2070 and ms73 lance
Anti-inflammatory effects of <t>LanC</t> in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected <t>by</t> <t>CCK-8</t> assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.
Sonopuls Equipped With Bandelin Uw2070 And Ms73 Lance, supplied by Bandelin Electronic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Anti-inflammatory effects of LanC in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected by CCK-8 assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Frontiers in Molecular Biosciences

Article Title: Network pharmacology analysis of Lanatoside C: molecular targets and mechanisms in the treatment of ulcerative colitis

doi: 10.3389/fmolb.2025.1552360

Figure Lengend Snippet: Anti-inflammatory effects of LanC in RAW264.7 cells. (A) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and cell viabilities were detected by CCK-8 assay. (B) RAW264.7 cells were treated with indicated concentrations of LanC for 24 h and IC50 of LanC were calculated with cell viability through CCK-8 assay. (C) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, supernatants were assayed by ELISA for levels of IL-1β, IL-6 and TNF-α. (D, E) RAW264.7 cells were pre-treated with 5 μM or 10 μM LanC for 1 h and stimulated with 1 μg/mL LPS for 24 h, mRNA were assayed by Real-time PCR for levels of IL-1β, IL-6, and TNF-α (D) or STAT3, KDR, ABCB1a, and ABCB1b (E) . Data were collected from three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following 24 h incubation, cells were treated with indicated concentrations of LanC (MedChemExpress) for 24 h. CCK-8 solution (MedChemExpress) was added to the wells and the plates were returned to the 37°C humidified chamber under a 5% CO 2 atmosphere for 1 h. The absorbance (A) values of cells were assessed at 450 nm using an enzyme marker, and the cell viability was computed.

Techniques: CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction