Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: Clinical characteristics of PCa patients and LanCL1 expression

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: a , b LanCL1 overexpression in LNCaP and PC-3 cells reduced cell death after 24 h treatment of 150 μM H2O2. Student’s t -test was performed for statistical significance analysis. N = 5. c , d LanCL1 knockdown increased LNCaP and PC-3 cells death induced by H 2 O 2 (100 μM H 2 O 2 , 24 h treatment). Student’s t -test was performed for statistical significance analysis. N = 5. e Hoechst staining shows that downregulation of LanCL1 increased LNCaP cell death(indicated by arrows) induced by H 2 O 2 , while LanCL1 overexpression reduced cell death. f Quantitation of the Hoechst staining of LNCaP cell treated by H 2 O 2 . Student’s t -test was performed for statistical significance analysis. N >3

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Over Expression, Knockdown, Staining, Quantitation Assay

Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: a Data from The Human Protein Atlas portal show LanCL1 expression in different tissues. Prostate is indicated by red arrow. b IHC data from The Human Protein Atlas portal show LanCL1 expression is higher in prostate cancer tissues than in normal tissues. c . Western blotting was used to detect the LanCL1 expression in human prostate cell lines. Tubulin served as the control. d Quantitation of relative expression of LanCL1 protein in different prostate cancer cell lines. e , f Increased expression markedly correlates with high Gleason score and tumor stage, data from the TCGA data set (queried from cBioPortal). LanCL1 expression is exon-normalized signal intensity. Mann–Whitney U -tests were performed to assess statistical significance for the comparisons between groups

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Expressing, Western Blot, Control, Quantitation Assay, MANN-WHITNEY

Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: a Representative immunohistochemistry of LanCL1 on benign prostatic epithelia (benign) and prostate cancer tissues (Gleason score 6 or 9). Student’s t-test was performed for statistical significance analysis. b Quantitation of protein expression of LanCL1 in benign or prostate cancer tissues. c Western blot analysis of LanCL1 expression in 15 pairs of nontumor tissues (P) and PCa tissues (T). Student’s t-test was performed for statistical significance analysis. N = 15. d Immunohistochemical staining for LanCL1 protein in the dorsolateral prostate of WT and TRAMP mice

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Immunohistochemistry, Quantitation Assay, Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: a Stably transfected LNCaP cells and PC-3 cells interfered with LanCL1 were established. LanCL1 was knockdown by siRNA as indicated. The efficiency of knockdown were examined by western blotting. Tubulin served as the control. b Cell proliferation was measured at the indicated time points by MTS colorimetric assay (absorbance at 490 nm (OD490). Student’s t -test was performed for statistical significance analysis. N >3. * P <0.05, ** P <0.01, *** P <0.001 c Tumor growth in LanCL1 overexpressed PC-3 cells and control cells was investigated by xenograft tumor models. Student’s t-test was performed for statistical significance analysis. N = 7. ** P <0.01, *** P <0.001. d The cell cycle distribution was analyzed by fluorescent-activated cell scanning (FACS) in LanCL1 overexpressed LNCaP cells. e Column chart indicates the mean and SEM of the S-phase fraction for each cell. Student’s t-test was performed for statistical significance analysis. N = 5

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Stable Transfection, Transfection, Knockdown, Western Blot, Control, Colorimetric Assay

Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: a qRT–PCR shows no induction of LanCL1 mRNA in H 2 O 2 -treated (100 μM, 1 h) LNCaP cells. Student’s t -test was performed for statistical significance analysis. N = 3. b , c Western blots and quantification show that H 2 O 2 -treatment did not induce LanCL1 protein expression. Student’s t-test was performed for statistical significance analysis. N = 5. d – f Western blots and quantification show an increase in the level of 4-HNE in the H 2 O 2 -treated (100 μM, 1 h) LNCaP cells, but LanCL1 overexpression did not influence the level of 4-HNE. Western blots show that LanCL1 overexpression in LNCaP cells is not more resistant to H 2 O 2 -induced 4-HNE accumulation. Student’s t-test was performed for statistical significance analysis. N = 5. g ROS determination by using a fluorescent probe DCFH-DA in LNCaP and PC-3 cells untreated and treated with H 2 O 2 (100 μM, 1 h). Student’s t-test was performed for statistical significance analysis. N = 3

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Over Expression

Journal: Cell Death & Disease

Article Title: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

doi: 10.1038/s41419-017-0207-0

Figure Lengend Snippet: a , b Modulation of apoptosis related signaling proteins using antibody array assay. Total protein was extracted from LNCaP-NEO and LNCaP-LanCL1 cells. A slide-based antibody array was used for simultaneous detection of 19 signaling molecules involved in stress response and apoptosis using a PathScan Stress and Apoptosis Signaling Array kit. Each protein was arranged in duplicate. c The proteins with changes concentration included pP44/42 MAPK (ERK1/2) (Thr202/Try204), pAkt(S473), pHSP27(Ser82), pSmad2(Ser465), pP53(Ser15), pP38 MAPK(Thr180/Try182), pSAPK/JNK(Thr183/Try185) and Cleaved-Caspase-3(Asp175), which were indicated on the images, and quantified. d The concentrations of the protein in c were quantified. Student’s t -test was performed for statistical significance analysis. N = 3. Data are presented as mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001. e Luciferase assay shows that overexpression of LanCL1 reduced AP-1 transcription. Student’s t-test was performed for statistical significance analysis. N = 3. *** P <0.001 f Western blotting indicated the protein level of pSAPK/JNK(Thr183/Try185) in overexpression LNCaP and PC-3 cells. Student’s t -test was performed for statistical significance analysis. N = 3. g Western blotting indicated the protein level of pSAPK/JNK(Thr183/Try185) in overexpression LNCaP cells after 100 μM H 2 O 2 for 2 h. Student’s t-test was performed for statistical significance analysis. N = 3. H. SP600125 partially rescued the cell death caused by ROS in LanCL1 knockdown LNCaP cells. Student’s t-test was performed for statistical significance analysis. N = 5

Article Snippet: The primary antibody was LanCL1 (Proteintech, 1/600, 30 min).

Techniques: Ab Array, Concentration Assay, Luciferase, Over Expression, Western Blot, Knockdown

Journal: Frontiers in Plant Science

Article Title: Utilization of Transcriptome, Small RNA, and Degradome Sequencing to Provide Insights Into Drought Stress and Rewatering Treatment in Medicago ruthenica

doi: 10.3389/fpls.2021.675903

Figure Lengend Snippet: The miRNA-target gene pairs showed reverse expression patterns in the three comparison groups.

Article Snippet: , mtr-MIR2603-p3_2ss9AC17AC , ↑ , TRINITY_DN22815_c0_g1 , ↓ , lanC-like protein GCL1 ( Medicago truncatula ).

Techniques: Expressing, Comparison, Ubiquitin Proteomics, Sequencing