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dapt  (MedChemExpress)


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  • 97

    Structured Review

    MedChemExpress dapt
    Dapt, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt/DAPT/pm42854987-49-0-3
    Average 97 stars, based on 159 article reviews
    dapt - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    other:

    Article Title: Subnuclear genome compartmentalization controls bivalent chromatin activity.
    Article Snippet: For neuronal differentiation, NPCs were dissociated with Accutase and seeded at a density of 275,000 cells per cm2 on plates coated with 100 μg ml−1 poly-l-ornithine, 100 μg ml−1 poly-d-lysine, 10 μg ml−1 laminin and 10 μg ml−1 fibronectin (Sigma-Aldrich) in neuronal differentiation medium (Neurobasal-A supplemented with 2% B-27, 2 mM l-glutamine (Gibco), 200 μM l-ascorbic acid 2-phosphate, 10 μM dibutyryl-cyclic AMP (Sigma-Aldrich), 10 ng ml−1 BDNF (Peprotech), 10 ng ml−1 GDNF (Peprotech) and 10 μM DAPT (MedChemExpress)).

    Concentration Assay:

    Article Title: Notch signaling regulates corpus cavernosum fibroblast function via FLNA-associated cell adhesion
    Article Snippet: .. Cell treatment Human CCFs were treated with DAPT (40 micromolar; Notch inhibitor; HY-13027, MedChem Express), Jagged-1 (40 micromolar; Notch activator; HY-P1846A, MedChem Express), or an equivalent concentration of vehicle control (DMSO) for 48 h. Treatment medium was refreshed every 24 h to maintain consistent drug exposure. ..

    Control:

    Article Title: Notch signaling regulates corpus cavernosum fibroblast function via FLNA-associated cell adhesion
    Article Snippet: .. Cell treatment Human CCFs were treated with DAPT (40 micromolar; Notch inhibitor; HY-13027, MedChem Express), Jagged-1 (40 micromolar; Notch activator; HY-P1846A, MedChem Express), or an equivalent concentration of vehicle control (DMSO) for 48 h. Treatment medium was refreshed every 24 h to maintain consistent drug exposure. ..

    Immunofluorescence:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: The medium was refreshed every 48 h. Organoid morphology and the number of buds were monitored using an Olympus CKX53 microscope. .. For immunofluorescence analysis, organoids derived from overfed mice were seeded in 8-well culture slides (BD Falcon), cultured for 2 days, and then treated with 10μM DAPT (MedChemExpress, HY13027) for 4 days. .. Organoids derived from normal-fed mice were cultured for 4 days post-seeding and then treated with the glutamine transporter inhibitor S-benzyl-L-cysteine (MACKLIN, S802600) for an additional 2 days.

    Analysis:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: The medium was refreshed every 48 h. Organoid morphology and the number of buds were monitored using an Olympus CKX53 microscope. .. For immunofluorescence analysis, organoids derived from overfed mice were seeded in 8-well culture slides (BD Falcon), cultured for 2 days, and then treated with 10μM DAPT (MedChemExpress, HY13027) for 4 days. .. Organoids derived from normal-fed mice were cultured for 4 days post-seeding and then treated with the glutamine transporter inhibitor S-benzyl-L-cysteine (MACKLIN, S802600) for an additional 2 days.

    Organoids:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: The medium was refreshed every 48 h. Organoid morphology and the number of buds were monitored using an Olympus CKX53 microscope. .. For immunofluorescence analysis, organoids derived from overfed mice were seeded in 8-well culture slides (BD Falcon), cultured for 2 days, and then treated with 10μM DAPT (MedChemExpress, HY13027) for 4 days. .. Organoids derived from normal-fed mice were cultured for 4 days post-seeding and then treated with the glutamine transporter inhibitor S-benzyl-L-cysteine (MACKLIN, S802600) for an additional 2 days.

    Derivative Assay:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: The medium was refreshed every 48 h. Organoid morphology and the number of buds were monitored using an Olympus CKX53 microscope. .. For immunofluorescence analysis, organoids derived from overfed mice were seeded in 8-well culture slides (BD Falcon), cultured for 2 days, and then treated with 10μM DAPT (MedChemExpress, HY13027) for 4 days. .. Organoids derived from normal-fed mice were cultured for 4 days post-seeding and then treated with the glutamine transporter inhibitor S-benzyl-L-cysteine (MACKLIN, S802600) for an additional 2 days.

    Cell Culture:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: The medium was refreshed every 48 h. Organoid morphology and the number of buds were monitored using an Olympus CKX53 microscope. .. For immunofluorescence analysis, organoids derived from overfed mice were seeded in 8-well culture slides (BD Falcon), cultured for 2 days, and then treated with 10μM DAPT (MedChemExpress, HY13027) for 4 days. .. Organoids derived from normal-fed mice were cultured for 4 days post-seeding and then treated with the glutamine transporter inhibitor S-benzyl-L-cysteine (MACKLIN, S802600) for an additional 2 days.



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