dapt Search Results


dapt  (Tocris)
94
Tocris dapt
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
Dapt, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapt/product/Tocris
Average 94 stars, based on 1 article reviews
dapt - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
ldn193189  (MedChemExpress)
95
MedChemExpress ldn193189
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
Ldn193189, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ldn193189/product/MedChemExpress
Average 95 stars, based on 1 article reviews
ldn193189 - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier
γ secretase inhibitor n  (MedChemExpress)
95
MedChemExpress γ secretase inhibitor n
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
γ Secretase Inhibitor N, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/γ secretase inhibitor n/product/MedChemExpress
Average 95 stars, based on 1 article reviews
γ secretase inhibitor n - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier
dapt  (Selleck Chemicals)
96
Selleck Chemicals dapt
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
Dapt, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapt/product/Selleck Chemicals
Average 96 stars, based on 1 article reviews
dapt - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
3 5 difluorophenacetyl l alanyl s phenylglycine t butyl ester  (StressMarq)
90
StressMarq 3 5 difluorophenacetyl l alanyl s phenylglycine t butyl ester
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
3 5 Difluorophenacetyl L Alanyl S Phenylglycine T Butyl Ester, supplied by StressMarq, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 5 difluorophenacetyl l alanyl s phenylglycine t butyl ester/product/StressMarq
Average 90 stars, based on 1 article reviews
3 5 difluorophenacetyl l alanyl s phenylglycine t butyl ester - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
dapt  (Thermo Fisher)
95
Thermo Fisher dapt
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
Dapt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapt/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
dapt - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier
2015 n ◦ 180 15 meemf sg dgf dapt scbt  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology 2015 n ◦ 180 15 meemf sg dgf dapt scbt
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
2015 N ◦ 180 15 Meemf Sg Dgf Dapt Scbt, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2015 n ◦ 180 15 meemf sg dgf dapt scbt/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
2015 n ◦ 180 15 meemf sg dgf dapt scbt - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
dapt  (TargetMol)
94
TargetMol dapt
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
Dapt, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapt/product/TargetMol
Average 94 stars, based on 1 article reviews
dapt - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
stem cell signaling inhibitors dapt  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc stem cell signaling inhibitors dapt
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
Stem Cell Signaling Inhibitors Dapt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stem cell signaling inhibitors dapt/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
stem cell signaling inhibitors dapt - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
n n  (Biosynth Carbosynth)
90
Biosynth Carbosynth n n
Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, <t>TGFβ3,</t> <t>dbcAMP,</t> <t>DAPT.</t> (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.
N N, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n n/product/Biosynth Carbosynth
Average 90 stars, based on 1 article reviews
n n - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, TGFβ3, dbcAMP, DAPT. (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Generation of hiPSC-Derived Functional Dopaminergic Neurons in Alginate-Based 3D Culture

doi: 10.3389/fcell.2021.708389

Figure Lengend Snippet: Viability of hiPSCs encapsulated and differentiated in alginate of different compositions. (A) The induction of early neuroectoderm differentiation from hiPSCs was achieved by dual SMAD inhibition, followed by the activation of SHH, Wnt, and FGF8 signaling pathways for patterning the midbrain fate. The committed neural progenitor cells were terminally differentiated into DA neurons by withdrawal of key neurogenic factors (BAGTCD). SM, StemMACS medium; RI, Rho associated kinase inhibitor; BAGTCD, BDNF, L-Ascorbic Acid, GDNF, TGFβ3, dbcAMP, DAPT. (B) Representative 3D reconstructions of cell aggregates stained with Calcein-AM (green, live cells) and Ethidium Homodimer-1 (red, dead cells). Scale bar represents 100 μm. (C) Cell viability over time was calculated as a percentage of green/red ratio. (D) Average size of the cell aggregates formed by viable cells was measured in millions of cubic micrometers. Statistical differences were calculated by two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ** p ≤ 0.005.

Article Snippet: On day 12, maturation of DA neurons was initiated by adding recombinant Human BDNF (Peprotech), recombinant Human GDNF (Peprotech), ascorbic acid (AA, Sigma), recombinant Human TGF-beta 3 (β3, Peprotech), dibutyryl-cyclic-AMP (dbcAMP, EnzoLifescience) and DAPT (Tocris).

Techniques: Inhibition, Activation Assay, Protein-Protein interactions, Staining