Journal: ACS Omega
Article Title: A LRELHLNNN-Gal‑3 Modified Adipose Decellularized Scaffold To Construct Colorectal Cancer Model for Anticancer Drug Screening and Immunotherapy
doi: 10.1021/acsomega.5c07342
Figure Lengend Snippet: Evaluation of chemotherapy resistance in LoVo cells cultured in LRELHLNNN-Gal-3 modified adECM scaffolds. (A) Apoptosis results of LoVo cells seeded in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds after treatment with 5-Fu for 12, 24, and 48 h. (B) Results for oxaliplatin treatment. (C) Results for capecitabine treatment. All samples were analyzed by CCK-8 assay. (D) Flow cytometry analysis of cell viability after 48 h treatment with 5-Fu (E), oxaliplatin (F), and capecitabine (G) in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds. Data are presented as mean ± SD ( n = 3). Statistical significance: n.s. = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: The sensitivity of LoVo cells to chemotherapeutic drugs was assessed using 5-Fu (100 μM, MedChemExpress, HY-90006), oxaliplatin (20 μM, MedChemExpress, HY-17371), and capecitabine (1.7 mM, MedChemExpress, HY-B0016, Obtained from the IC50 curve of the drug, Figure S1 ) as model drugs under different culture conditions.
Techniques: Cell Culture, Modification, CCK-8 Assay, Flow Cytometry