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Evaluation of chemotherapy resistance in LoVo cells cultured in LRELHLNNN-Gal-3 modified adECM scaffolds. (A) Apoptosis results of LoVo cells seeded in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds after treatment with 5-Fu for 12, 24, and 48 h. (B) Results for oxaliplatin treatment. (C) Results for <t>capecitabine</t> treatment. All samples were analyzed by CCK-8 assay. (D) Flow cytometry analysis of cell viability after 48 h treatment with 5-Fu (E), oxaliplatin (F), and capecitabine (G) in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds. Data are presented as mean ± SD ( n = 3). Statistical significance: n.s. = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Evaluation of chemotherapy resistance in LoVo cells cultured in LRELHLNNN-Gal-3 modified adECM scaffolds. (A) Apoptosis results of LoVo cells seeded in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds after treatment with 5-Fu for 12, 24, and 48 h. (B) Results for oxaliplatin treatment. (C) Results for <t>capecitabine</t> treatment. All samples were analyzed by CCK-8 assay. (D) Flow cytometry analysis of cell viability after 48 h treatment with 5-Fu (E), oxaliplatin (F), and capecitabine (G) in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds. Data are presented as mean ± SD ( n = 3). Statistical significance: n.s. = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Evaluation of chemotherapy resistance in LoVo cells cultured in LRELHLNNN-Gal-3 modified adECM scaffolds. (A) Apoptosis results of LoVo cells seeded in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds after treatment with 5-Fu for 12, 24, and 48 h. (B) Results for oxaliplatin treatment. (C) Results for <t>capecitabine</t> treatment. All samples were analyzed by CCK-8 assay. (D) Flow cytometry analysis of cell viability after 48 h treatment with 5-Fu (E), oxaliplatin (F), and capecitabine (G) in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds. Data are presented as mean ± SD ( n = 3). Statistical significance: n.s. = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Dose-dependent effects of <t>capecitabine,</t> acyclovir and lamivudine on cell viability analyzed via non-linear regression. Cell viability (%) following treatment with (A) capecitabine, (B) acyclovir and (C) lamivudine. (D) Non-linear regression curves were used to determine the association between cell activity (%) and capecitabine, aciclovir and lamivudine concentration [Log (µM)].
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Dose-dependent effects of <t>capecitabine,</t> acyclovir and lamivudine on cell viability analyzed via non-linear regression. Cell viability (%) following treatment with (A) capecitabine, (B) acyclovir and (C) lamivudine. (D) Non-linear regression curves were used to determine the association between cell activity (%) and capecitabine, aciclovir and lamivudine concentration [Log (µM)].
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Evaluation of chemotherapy resistance in LoVo cells cultured in LRELHLNNN-Gal-3 modified adECM scaffolds. (A) Apoptosis results of LoVo cells seeded in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds after treatment with 5-Fu for 12, 24, and 48 h. (B) Results for oxaliplatin treatment. (C) Results for capecitabine treatment. All samples were analyzed by CCK-8 assay. (D) Flow cytometry analysis of cell viability after 48 h treatment with 5-Fu (E), oxaliplatin (F), and capecitabine (G) in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds. Data are presented as mean ± SD ( n = 3). Statistical significance: n.s. = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: ACS Omega

Article Title: A LRELHLNNN-Gal‑3 Modified Adipose Decellularized Scaffold To Construct Colorectal Cancer Model for Anticancer Drug Screening and Immunotherapy

doi: 10.1021/acsomega.5c07342

Figure Lengend Snippet: Evaluation of chemotherapy resistance in LoVo cells cultured in LRELHLNNN-Gal-3 modified adECM scaffolds. (A) Apoptosis results of LoVo cells seeded in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds after treatment with 5-Fu for 12, 24, and 48 h. (B) Results for oxaliplatin treatment. (C) Results for capecitabine treatment. All samples were analyzed by CCK-8 assay. (D) Flow cytometry analysis of cell viability after 48 h treatment with 5-Fu (E), oxaliplatin (F), and capecitabine (G) in 2D culture, LRELHLNNN-Gal-3 modified adECM scaffolds, and Gal-3 modified adECM scaffolds. Data are presented as mean ± SD ( n = 3). Statistical significance: n.s. = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The sensitivity of LoVo cells to chemotherapeutic drugs was assessed using 5-Fu (100 μM, MedChemExpress, HY-90006), oxaliplatin (20 μM, MedChemExpress, HY-17371), and capecitabine (1.7 mM, MedChemExpress, HY-B0016, Obtained from the IC50 curve of the drug, Figure S1 ) as model drugs under different culture conditions.

Techniques: Cell Culture, Modification, CCK-8 Assay, Flow Cytometry

Dose-dependent effects of capecitabine, acyclovir and lamivudine on cell viability analyzed via non-linear regression. Cell viability (%) following treatment with (A) capecitabine, (B) acyclovir and (C) lamivudine. (D) Non-linear regression curves were used to determine the association between cell activity (%) and capecitabine, aciclovir and lamivudine concentration [Log (µM)].

Journal: Molecular Medicine Reports

Article Title: Identification of metabolites associated with capecitabine-induced hand-foot syndrome using untargeted metabolomics in patients with cancer

doi: 10.3892/mmr.2025.13568

Figure Lengend Snippet: Dose-dependent effects of capecitabine, acyclovir and lamivudine on cell viability analyzed via non-linear regression. Cell viability (%) following treatment with (A) capecitabine, (B) acyclovir and (C) lamivudine. (D) Non-linear regression curves were used to determine the association between cell activity (%) and capecitabine, aciclovir and lamivudine concentration [Log (µM)].

Article Snippet: All patients received oral capecitabine (cat. no. 1D0733DE3; Qilu Pharmaceutical Co., Ltd.) for chemotherapy.

Techniques: Activity Assay, Concentration Assay

HaCaT cell proliferation following treatment. **P<0.01 vs. control; ## P<0.01 vs. 50% capecitabine. HaCaT, human adult keratinocyte cell line, TPA-treated cells.

Journal: Molecular Medicine Reports

Article Title: Identification of metabolites associated with capecitabine-induced hand-foot syndrome using untargeted metabolomics in patients with cancer

doi: 10.3892/mmr.2025.13568

Figure Lengend Snippet: HaCaT cell proliferation following treatment. **P<0.01 vs. control; ## P<0.01 vs. 50% capecitabine. HaCaT, human adult keratinocyte cell line, TPA-treated cells.

Article Snippet: All patients received oral capecitabine (cat. no. 1D0733DE3; Qilu Pharmaceutical Co., Ltd.) for chemotherapy.

Techniques: Control

Calcein acetoxymethyl ester/propidium iodide double staining of HaCaT cells. *P<0.05, **P<0.01 vs. control; ## P<0.01 vs. 50% capecitabine. HaCaT, human adult keratinocyte cell line, TPA-treated cells.

Journal: Molecular Medicine Reports

Article Title: Identification of metabolites associated with capecitabine-induced hand-foot syndrome using untargeted metabolomics in patients with cancer

doi: 10.3892/mmr.2025.13568

Figure Lengend Snippet: Calcein acetoxymethyl ester/propidium iodide double staining of HaCaT cells. *P<0.05, **P<0.01 vs. control; ## P<0.01 vs. 50% capecitabine. HaCaT, human adult keratinocyte cell line, TPA-treated cells.

Article Snippet: All patients received oral capecitabine (cat. no. 1D0733DE3; Qilu Pharmaceutical Co., Ltd.) for chemotherapy.

Techniques: Double Staining, Control