Journal: Nature Communications
Article Title: Heterobifunctional proteomimetic polymers for targeted degradation of MYC and KRAS
doi: 10.1038/s41467-026-68913-3
Figure Lengend Snippet: Differential cell toxicity following treatment with HYDRAC or P-sHR. Representative dose-response plots of PC3 ( a ), A549 ( b ), or MycCaP ( c ) cells following 72 h treatment with viability normalized to vehicle-treated control from n = 3 technical replicates. d Summary IC 50 values in PC3 and A549 cells following treatment with indicated polymers averaged from n = 3 independent biological experiments with > 2 different batch formulations. e Annexin V/PI staining of PC3 cells treated for 24 h with HYDRAC analyzed by flow cytometry and split into late (Annexin V-FITC + /PI + ) and early (Annexin V-FITC + /PI + ) stage apoptosis. The DMSO group was incubated with 10% DMSO as a positive control. n = 3 independent samples per group. Experiment repeated with similar results. f Representative dose-response curves of PC3 cells treated for 72 h with HYDRACs consisting of different targeting ligand to degron ratios with viability normalized to vehicle-treated controls. g PC3 (solid) or PC12 (dashed) cells were treated with HYDRAC for 72 h and cell viability was normalized to vehicle treatment. IC 50 values listed. h A549 cells were incubated with a single treatment of 10 or 20 μM of the indicated polymer formulations at day 0, and cell counts were normalized to the initial seeding amount monitored over 2 weeks. Data depict mean ± s.d. P -values determined by one-way ANOVA in d, e . One representative dose-response or proliferation curve from n = 3 independent experiments is shown in ( f), ( g ), and ( h ). * P < 0.05, ** P < 0.01, ns: not significant.
Article Snippet: PC-3, PC-12, A549, 293T, and HCT116 cells were obtained from ATCC; Mouse MycCaP cells were the kind gift of Charles Sawyers (Memorial Sloan-Kettering Cancer Centre); HCT116 KEAP1 KO cells were obtained from Abcam (ab286484); 293 T VHL KO cells were obtained from Creative Biogene (CSC-RT2714).
Techniques: Control, Staining, Flow Cytometry, Incubation, Positive Control, Polymer
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