eukaryotic expression vector psectag2b (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
86
Structured Review
Thermo Fisher
eukaryotic expression vector psectag2b
Eukaryotic Expression Vector Psectag2b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psectag2b+expression+vector/pm37752138-83-4-8
Average 86 stars, based on 1 article reviews
Eukaryotic Expression Vector Psectag2b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psectag2b+expression+vector/pm37752138-83-4-8
Average 86 stars, based on 1 article reviews
eukaryotic expression vector psectag2b - by Bioz Stars,
2026-09
86/100 stars
Images
Related Articles
Amplification:Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity. Article Snippet: .. The amplified flaB and Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity Article Snippet: .. The amplified flaB and Expressing:Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity. Article Snippet: .. The amplified flaB and Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2 Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody Sigma-Aldrich Cat# A8592-1MG; RRID: AB_439702 Anti-M13-horseradish peroxidase (HRP) polyclonal antibody Pharmacia Cat# 27-9421 CR3022 scFv This paper N/A Bacterial and Virus Strains TG1 Electrocompetent Cells Lucigen Cat# 60502-1 HB2151 Escherichia coli Strains This paper N/A SARS-CoV-2 strain nCoV-SH01 Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Phosphate Buffered Saline solution HyClone Cat# SH30256.01B Ni Sepharose High Performance GE Healthcare Cat# 17526802 Protein G Sepharose 4 Fast Flow resin GE Healthcare Cat# 17061805 BirA500 Kit Avidity N/A Polymyxin B sulfate salt Sigma-Aldrich Cat# P1004-5MU High Fidelity PCR Master Roche Cat# 12140314001 SfiI New England Biolabs Cat# R0123L Dynabeads MyOne Streptavidin T1 Thermo Fisher Cat# 65601 ABTS Thermo Fisher Cat# 002024 EZ-Link Sulfo-NHS-LC-LC-Biotin Thermo Fisher Cat# A35358 Biotinylated ZIKV DIII This paper N/A Biotinylated Human TIM-3 / HAVCR2 ACROBiosystems Cat# TM3-H82E7 Human IL-33 ACROBiosystems Cat# IL3-H52H7 Human TPBG / 5T4 ACROBiosystems Cat# TPG-H52E5 Human TNF-a Sino Biological Inc. Cat# 10602-HNAE SARS-CoV-2 Spike S1-His Sino Biological Inc. Cat# 40591-V08H SARS-CoV-2 Spike RBD-His Sino Biological Inc. Cat# 40592-V08B ACE2-His Protein Novoprotein Scientific Inc. Cat# C419 Critical Commercial Assays Bright-Glo Luciferase Assay System Promega Cat# E2620 Cell Couting Kit-8 Assay Kit Dojindo Cat# CK04-20 Experimental Models: Cell Lines Expi293 Expression System Thermo Fisher Cat# A14635 Vero E6 cells ATCC Cat# CRL-1586 Recombinant DNA Germline IGHV alleles genes Genscript N/A VHH#3 gene Genscript N/A RBD gene: SARS-CoV-2 (strain WIV04, GISAID accession number EPI_ISL_402124) Genscript N/A RBD gene: SARS-CoV-2 (strain SZTH-004, GISAID accession number EPI_ISL_406595) Genscript N/A (Continued on next page) Cell Host & Microbe 27, 891–898.e1–e5, June 10, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER RBD gene: SARS-CoV-2 (strain IDF0372, GISAID accession number EPI_ISL_406596) Genscript N/A RBD gene: SARS-CoV (strain WH20) Genscript N/A pComb3x vector Addgene Cat# 63891 pSecTag2B expression vector Thermo Fisher Cat# Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity Article Snippet: .. The amplified flaB and Plasmid Preparation:Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity. Article Snippet: .. The amplified flaB and Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Article Title: Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc) Article Snippet: .. Genes encoding the different polypeptide chains were cloned into the Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Article Title: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells Article Snippet: .. To generate the scDb, variable domains of the heavy and light chains of the 3–43 and a humanized version of UCHT1 (huU3) were cloned into the Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity Article Snippet: .. The amplified flaB and Gel Extraction:Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Polymerase Chain Reaction:Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Purification:Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Clone Assay:Article Title: Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc) Article Snippet: .. Genes encoding the different polypeptide chains were cloned into the Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Article Title: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells Article Snippet: .. To generate the scDb, variable domains of the heavy and light chains of the 3–43 and a humanized version of UCHT1 (huU3) were cloned into the Modification:Article Title: Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc) Article Snippet: .. Genes encoding the different polypeptide chains were cloned into the Article Title: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells Article Snippet: .. To generate the scDb, variable domains of the heavy and light chains of the 3–43 and a humanized version of UCHT1 (huU3) were cloned into the Software:Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2 Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody Sigma-Aldrich Cat# A8592-1MG; RRID: AB_439702 Anti-M13-horseradish peroxidase (HRP) polyclonal antibody Pharmacia Cat# 27-9421 CR3022 scFv This paper N/A Bacterial and Virus Strains TG1 Electrocompetent Cells Lucigen Cat# 60502-1 HB2151 Escherichia coli Strains This paper N/A SARS-CoV-2 strain nCoV-SH01 Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Phosphate Buffered Saline solution HyClone Cat# SH30256.01B Ni Sepharose High Performance GE Healthcare Cat# 17526802 Protein G Sepharose 4 Fast Flow resin GE Healthcare Cat# 17061805 BirA500 Kit Avidity N/A Polymyxin B sulfate salt Sigma-Aldrich Cat# P1004-5MU High Fidelity PCR Master Roche Cat# 12140314001 SfiI New England Biolabs Cat# R0123L Dynabeads MyOne Streptavidin T1 Thermo Fisher Cat# 65601 ABTS Thermo Fisher Cat# 002024 EZ-Link Sulfo-NHS-LC-LC-Biotin Thermo Fisher Cat# A35358 Biotinylated ZIKV DIII This paper N/A Biotinylated Human TIM-3 / HAVCR2 ACROBiosystems Cat# TM3-H82E7 Human IL-33 ACROBiosystems Cat# IL3-H52H7 Human TPBG / 5T4 ACROBiosystems Cat# TPG-H52E5 Human TNF-a Sino Biological Inc. Cat# 10602-HNAE SARS-CoV-2 Spike S1-His Sino Biological Inc. Cat# 40591-V08H SARS-CoV-2 Spike RBD-His Sino Biological Inc. Cat# 40592-V08B ACE2-His Protein Novoprotein Scientific Inc. Cat# C419 Critical Commercial Assays Bright-Glo Luciferase Assay System Promega Cat# E2620 Cell Couting Kit-8 Assay Kit Dojindo Cat# CK04-20 Experimental Models: Cell Lines Expi293 Expression System Thermo Fisher Cat# A14635 Vero E6 cells ATCC Cat# CRL-1586 Recombinant DNA Germline IGHV alleles genes Genscript N/A VHH#3 gene Genscript N/A RBD gene: SARS-CoV-2 (strain WIV04, GISAID accession number EPI_ISL_402124) Genscript N/A RBD gene: SARS-CoV-2 (strain SZTH-004, GISAID accession number EPI_ISL_406595) Genscript N/A (Continued on next page) Cell Host & Microbe 27, 891–898.e1–e5, June 10, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER RBD gene: SARS-CoV-2 (strain IDF0372, GISAID accession number EPI_ISL_406596) Genscript N/A RBD gene: SARS-CoV (strain WH20) Genscript N/A pComb3x vector Addgene Cat# 63891 pSecTag2B expression vector Thermo Fisher Cat# Construct:Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the |