psectag2b expression vector (Thermo Fisher)
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psectag2b expression vector
Psectag2b Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psectag2b+expression+vector/pm36652482-237-7-11
Average 86 stars, based on 1 article reviews
Psectag2b Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psectag2b+expression+vector/pm36652482-237-7-11
Average 86 stars, based on 1 article reviews
psectag2b expression vector - by Bioz Stars,
2026-09
86/100 stars
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Amplification:Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity. Article Snippet: .. The amplified flaB and Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity Article Snippet: .. The amplified flaB and Expressing:Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity. Article Snippet: .. The amplified flaB and Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2 Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody Sigma-Aldrich Cat# A8592-1MG; RRID: AB_439702 Anti-M13-horseradish peroxidase (HRP) polyclonal antibody Pharmacia Cat# 27-9421 CR3022 scFv This paper N/A Bacterial and Virus Strains TG1 Electrocompetent Cells Lucigen Cat# 60502-1 HB2151 Escherichia coli Strains This paper N/A SARS-CoV-2 strain nCoV-SH01 Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Phosphate Buffered Saline solution HyClone Cat# SH30256.01B Ni Sepharose High Performance GE Healthcare Cat# 17526802 Protein G Sepharose 4 Fast Flow resin GE Healthcare Cat# 17061805 BirA500 Kit Avidity N/A Polymyxin B sulfate salt Sigma-Aldrich Cat# P1004-5MU High Fidelity PCR Master Roche Cat# 12140314001 SfiI New England Biolabs Cat# R0123L Dynabeads MyOne Streptavidin T1 Thermo Fisher Cat# 65601 ABTS Thermo Fisher Cat# 002024 EZ-Link Sulfo-NHS-LC-LC-Biotin Thermo Fisher Cat# A35358 Biotinylated ZIKV DIII This paper N/A Biotinylated Human TIM-3 / HAVCR2 ACROBiosystems Cat# TM3-H82E7 Human IL-33 ACROBiosystems Cat# IL3-H52H7 Human TPBG / 5T4 ACROBiosystems Cat# TPG-H52E5 Human TNF-a Sino Biological Inc. Cat# 10602-HNAE SARS-CoV-2 Spike S1-His Sino Biological Inc. Cat# 40591-V08H SARS-CoV-2 Spike RBD-His Sino Biological Inc. Cat# 40592-V08B ACE2-His Protein Novoprotein Scientific Inc. Cat# C419 Critical Commercial Assays Bright-Glo Luciferase Assay System Promega Cat# E2620 Cell Couting Kit-8 Assay Kit Dojindo Cat# CK04-20 Experimental Models: Cell Lines Expi293 Expression System Thermo Fisher Cat# A14635 Vero E6 cells ATCC Cat# CRL-1586 Recombinant DNA Germline IGHV alleles genes Genscript N/A VHH#3 gene Genscript N/A RBD gene: SARS-CoV-2 (strain WIV04, GISAID accession number EPI_ISL_402124) Genscript N/A RBD gene: SARS-CoV-2 (strain SZTH-004, GISAID accession number EPI_ISL_406595) Genscript N/A (Continued on next page) Cell Host & Microbe 27, 891–898.e1–e5, June 10, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER RBD gene: SARS-CoV-2 (strain IDF0372, GISAID accession number EPI_ISL_406596) Genscript N/A RBD gene: SARS-CoV (strain WH20) Genscript N/A pComb3x vector Addgene Cat# 63891 pSecTag2B expression vector Thermo Fisher Cat# Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity Article Snippet: .. The amplified flaB and Plasmid Preparation:Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity. Article Snippet: .. The amplified flaB and Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Article Title: Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc) Article Snippet: .. Genes encoding the different polypeptide chains were cloned into the Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Article Title: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells Article Snippet: .. To generate the scDb, variable domains of the heavy and light chains of the 3–43 and a humanized version of UCHT1 (huU3) were cloned into the Article Title: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity Article Snippet: .. The amplified flaB and Gel Extraction:Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Polymerase Chain Reaction:Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Purification:Article Title: Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells. Article Snippet: Background Interleukin-21 (IL-21) is a cytokine produced by various cell types, including T cells, natural killer cells, myeloid cells, and B cells, and has a broad range of potential applications in cancer therapy.. To improve the therapeutic index, we explored the use of fusion technologies that involved linking other anticancer peptides to the IL-21 gene using specific linkers.. Objectives This study aimed to compare the anticancer potential of IL-21 and IL-21 fusion proteins. Clone Assay:Article Title: Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc) Article Snippet: .. Genes encoding the different polypeptide chains were cloned into the Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer. Article Snippet: .. The PCR product was cloned into a Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the Article Title: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells Article Snippet: .. To generate the scDb, variable domains of the heavy and light chains of the 3–43 and a humanized version of UCHT1 (huU3) were cloned into the Modification:Article Title: Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc) Article Snippet: .. Genes encoding the different polypeptide chains were cloned into the Article Title: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells Article Snippet: .. To generate the scDb, variable domains of the heavy and light chains of the 3–43 and a humanized version of UCHT1 (huU3) were cloned into the Software:Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2 Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody Sigma-Aldrich Cat# A8592-1MG; RRID: AB_439702 Anti-M13-horseradish peroxidase (HRP) polyclonal antibody Pharmacia Cat# 27-9421 CR3022 scFv This paper N/A Bacterial and Virus Strains TG1 Electrocompetent Cells Lucigen Cat# 60502-1 HB2151 Escherichia coli Strains This paper N/A SARS-CoV-2 strain nCoV-SH01 Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Phosphate Buffered Saline solution HyClone Cat# SH30256.01B Ni Sepharose High Performance GE Healthcare Cat# 17526802 Protein G Sepharose 4 Fast Flow resin GE Healthcare Cat# 17061805 BirA500 Kit Avidity N/A Polymyxin B sulfate salt Sigma-Aldrich Cat# P1004-5MU High Fidelity PCR Master Roche Cat# 12140314001 SfiI New England Biolabs Cat# R0123L Dynabeads MyOne Streptavidin T1 Thermo Fisher Cat# 65601 ABTS Thermo Fisher Cat# 002024 EZ-Link Sulfo-NHS-LC-LC-Biotin Thermo Fisher Cat# A35358 Biotinylated ZIKV DIII This paper N/A Biotinylated Human TIM-3 / HAVCR2 ACROBiosystems Cat# TM3-H82E7 Human IL-33 ACROBiosystems Cat# IL3-H52H7 Human TPBG / 5T4 ACROBiosystems Cat# TPG-H52E5 Human TNF-a Sino Biological Inc. Cat# 10602-HNAE SARS-CoV-2 Spike S1-His Sino Biological Inc. Cat# 40591-V08H SARS-CoV-2 Spike RBD-His Sino Biological Inc. Cat# 40592-V08B ACE2-His Protein Novoprotein Scientific Inc. Cat# C419 Critical Commercial Assays Bright-Glo Luciferase Assay System Promega Cat# E2620 Cell Couting Kit-8 Assay Kit Dojindo Cat# CK04-20 Experimental Models: Cell Lines Expi293 Expression System Thermo Fisher Cat# A14635 Vero E6 cells ATCC Cat# CRL-1586 Recombinant DNA Germline IGHV alleles genes Genscript N/A VHH#3 gene Genscript N/A RBD gene: SARS-CoV-2 (strain WIV04, GISAID accession number EPI_ISL_402124) Genscript N/A RBD gene: SARS-CoV-2 (strain SZTH-004, GISAID accession number EPI_ISL_406595) Genscript N/A (Continued on next page) Cell Host & Microbe 27, 891–898.e1–e5, June 10, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER RBD gene: SARS-CoV-2 (strain IDF0372, GISAID accession number EPI_ISL_406596) Genscript N/A RBD gene: SARS-CoV (strain WH20) Genscript N/A pComb3x vector Addgene Cat# 63891 pSecTag2B expression vector Thermo Fisher Cat# Construct:Article Title: Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis Article Snippet: The TMEM67 cleavage site mutations (S1, S2, and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length, human TMEM67 plasmid construct in the pCDNA3.1Myc/His vector backbone, originally cloned by the Johnson lab [ ]. .. TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the |