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t4 dna ligase  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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    Structured Review

    New England Biolabs t4 dna ligase
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psectag2b+expression+vector/T4+DNA+Ligase/custom%40m0202%4010400683
    Average 99 stars, based on 33344 article reviews
    t4 dna ligase - by Bioz Stars, 2026-09
    99/100 stars

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    Construct:

    Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling
    Article Snippet: .. Vectors were pretreated with Antarctic phosphatase (M0289, NEB), and the DVL, CK1ε, or CK1δ constructs were ligated using T4 DNA Ligase (M0202, NEB). ..

    Ligation:

    Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation
    Article Snippet: .. 2000 units of T4 DNA ligase (NEB #M0202M) were used for ligation at 37°C for 1 h. RNA ligase 2 (NEB #M0239L) reactions were either set up with DNA splint or RNA splint. .. Reaction conditions remained the same for both the splints: 15 units of enzyme with 10% polyethylene gylcol (PEG), 100 mM MgCl 2 , and RNA ligase 2 buffer, and reactions were incubated at 25°C for 1 h. For RNA ligase 1 (NEB #M0204S) reactions, 25 units of the enzyme were added with 10% PEG, 1× RNA ligase buffer and incubated at 37°C for 1 h. When DNA splints were included, the addition of 2 units of Turbo DNase (Invitrogen #AM2238) for 15 min at 37°C degraded the DNA immediately after the end of ligation incubation.

    Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients
    Article Snippet: DNA (1 pg-200 ng) was digested with 4 U of BcgI (NEB) for 3 hours at 37 °C. .. Adaptors were then ligated to the DNA fragments by mixing 5 μl of digested DNA with 10 μl of a ligation master mix containing 0.2 μM of each adaptor and 800 U of T4 DNA ligase (NEB). ..

    Plasmid Preparation:

    Article Title: Novel ADPRS Missense Variant (p.Leu162Pro) Causes Stress-Induced Childhood-Onset Neurodegeneration With Ataxia and Seizures
    Article Snippet: .. These oligonucleotides were annealed and inserted into eSpCas9(1.1) vector (addgene #71814) using restriction enzyme BbsI and T4 DNA ligase from NEB (MA). .. Competent Escherichia coli cells (NEB® 5-alpha strain, MA) were transformed by heat shock and cloned vectors were purified by the Monarch® Plasmid Miniprep Kit (NEB, MA). hTERT-RPE1 cells were then transfected with this vector by electroporation, using NeonTM Transfection System (Thermo Fisher, MA), along with vector pCD2E (a kind gift of K. Caldecott, Univ.

    Amplification:

    Article Title: Elevated Expression of MALAT1 Contributes to the Survival of Drug-Tolerant Persister Cells Following Targeted Therapy in Lung Adenocarcinoma
    Article Snippet: .. The mCherry amplicon was then ligated into pSin-PuroR using T4 DNA ligase (New England Biolabs). ..

    Purification:

    Article Title: Poliovirus 3Dpol polymerase region is essential for cleavage of poliovirus 3AB in vivo.
    Article Snippet: The RT-PCR products were purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250). .. T4 DNA ligase buffer (NEB, #B0202S) (3 μL) and T4 DNA ligase (NEB, #M0202L) (800 U, 2 μL) were added to the purified RT-PCR products (25 μL). .. The samples were incubated at room temperature for 2 h for circularization and then purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Poliovirus 3Dpol polymerase region is essential for cleavage of poliovirus 3AB in vivo.
    Article Snippet: The RT-PCR products were purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250). .. T4 DNA ligase buffer (NEB, #B0202S) (3 μL) and T4 DNA ligase (NEB, #M0202L) (800 U, 2 μL) were added to the purified RT-PCR products (25 μL). .. The samples were incubated at room temperature for 2 h for circularization and then purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250).



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