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nebnext poly a mrna magnetic isolation module  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext poly a mrna magnetic isolation module
    Nebnext Poly A Mrna Magnetic Isolation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/module/NEBNext+Poly(A)+mRNA+Magnetic+Isolation+Module/pm42885819-58-8-8
    Average 99 stars, based on 6803 article reviews
    nebnext poly a mrna magnetic isolation module - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions.
    Article Snippet: Libraries were prepared using the NEBNext Ultra II Directional RNA-Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). .. PolyA mRNA was isolated using the Poly(A) mRNA Magnetic Isolation Module (NEB#E7490) (New England Biolabs, Ipswich, MA, USA) using the same DNase I treated RNA from the circ-RNA seq library prep. .. Prepared libraries were sequenced at GENEWIZ (Suzhou, China) using Illumina HiSeq 4000 Platform using the PE150 protocol.

    Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy.
    Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017.

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: RNA quality was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. For RNA-seq analysis, mRNA was captured using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Paired-end sequencing (150 base pairs) for three replicates was performed using Illumina NovaSeq X Plus (Illumina, San Diego, CA, USA).

    Article Title: The Maternal Blood Transcriptome Reflects Molecular Signatures Associated With Large Offspring Syndrome and Other Adverse Pregnancy Outcomes in Cattle
    Article Snippet: Sequencing was performed at the Interdisciplinary Center of Biotechnology Research NextGen Sequencing Core ( https://biotech.ufl.edu/next‐gen‐dna/ , RRID:SCR_019152). .. First, 500 ng of total RNA was used for mRNA isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA, catalog #E7490). .. Then, RNA library construction was performed with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, catalog #E7760) with 2/3 of recommended poly A enriched RNA and 1/4 recommended reaction volume using SPT Labtech mosquito LV liquid handling instrument (Tecan, Männedorf, Switzerland).

    Article Title: CG14075/Mmt promotes amino acid catabolism and cooperates with AKH during starvation in Drosophila.
    Article Snippet: RNA was extracted from ten CC-containing neuronal ganglia and wholebody samples using TRIzol (Thermo Fisher Scientific). .. RNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs [NEB], #E7490), followed by the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, #E7420). .. The samples were quantified by Agilent Bioanalyzer with Agilent High Sensitivity DNA kit (Agilent, #5067-4626).

    Article Title: The homologous GRAS transcription factors RAM1 and RAD1 coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis.
    Article Snippet: RNA-sequencing For all experiments, RNA samples were quality-checked using gel electrophoresis and bioanalyzer (Agilent, Santa Clara, CA, USA) .. For the AM experiment, mRNAs were enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA). .. Libraries were prepared using NEBNextUltra RNALibrary Prep Kit for Illumina (NEB) and sequenced on aNovaSeq 6000 S4 platform by BGI, Hong Kong (paired-end 150 bp).

    Article Title: Impact of environmental enrichment and allostatic load induced by repeated mixing on the transcriptome of three porcine brain tissues.
    Article Snippet: .. RNA was poly(A) enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), followed by library preparation with the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). .. Sequencing was conducted on the Illumina NovaSeq 6000 platform, generating stranded 150 bp paired-end reads.

    RNA Sequencing:

    Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy.
    Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017.

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: RNA quality was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. For RNA-seq analysis, mRNA was captured using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Paired-end sequencing (150 base pairs) for three replicates was performed using Illumina NovaSeq X Plus (Illumina, San Diego, CA, USA).

    Selection:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (NEB #E7490, Ipswich, MA, USA) for Illumina with poly(A) selection and subjected to 151-bp paired-end sequencing on an Illumina NovaSeq platform (San Diego, CA, USA), generating an average of approximately 40 million paired reads per sample. ..

    Sequencing:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (NEB #E7490, Ipswich, MA, USA) for Illumina with poly(A) selection and subjected to 151-bp paired-end sequencing on an Illumina NovaSeq platform (San Diego, CA, USA), generating an average of approximately 40 million paired reads per sample. ..

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: RNA quality was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. For RNA-seq analysis, mRNA was captured using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Paired-end sequencing (150 base pairs) for three replicates was performed using Illumina NovaSeq X Plus (Illumina, San Diego, CA, USA).

    Analysis:

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: RNA quality was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. For RNA-seq analysis, mRNA was captured using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Paired-end sequencing (150 base pairs) for three replicates was performed using Illumina NovaSeq X Plus (Illumina, San Diego, CA, USA).

    Purification:

    Article Title: CG14075/Mmt promotes amino acid catabolism and cooperates with AKH during starvation in Drosophila.
    Article Snippet: RNA was extracted from ten CC-containing neuronal ganglia and wholebody samples using TRIzol (Thermo Fisher Scientific). .. RNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs [NEB], #E7490), followed by the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, #E7420). .. The samples were quantified by Agilent Bioanalyzer with Agilent High Sensitivity DNA kit (Agilent, #5067-4626).



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    Image Search Results


    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: Libraries were prepared by blunting and dA-tailing the amplicons using the End Prep library kit (NEB; Ref: E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, Ref: E7595) with the adapters obtained from NEBNext Multiplex Oligos for Illumina (NEB, Ref:E7600) following standard protocols.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: End Prep library kit , NEB , E7546.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Journal: iScience

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

    doi: 10.1016/j.isci.2026.117719

    Figure Lengend Snippet:

    Article Snippet: Clampfit software , Molecular Devices , RRID: SCR_011323.

    Techniques: Cell-Signaling, Virus, Recombinant, Clinical Proteomics, Membrane, Staining, Cloning, Software