nebnext multiplex oligos for illumina (New England Biolabs)
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New England Biolabs
nebnext multiplex oligos for illumina
Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/module/NEBNext+Ultra+II+Ligation+Module/pmc13651819-675-34-34
Average 97 stars, based on 528 article reviews
Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/module/NEBNext+Ultra+II+Ligation+Module/pmc13651819-675-34-34
Average 97 stars, based on 528 article reviews
nebnext multiplex oligos for illumina - by Bioz Stars,
2026-11
97/100 stars
Images
1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"
Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses
Journal: iScience
doi: 10.1016/j.isci.2026.117639
Figure Legend Snippet:
Techniques Used: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software
Related Articles
Ligation:Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity Article Snippet: .. Ligation was carried out using the Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing Article Snippet: The mix of NTPs was composed of 40 μL of the 10 mM dATP, 4 μL of the 10 mM dCTP, and 4 μL of the 10 mM dGTP solution (N0446S, NEB). .. Finally, we ligated 1 μL of adapter B using Article Title: Quantifying replication fidelity of unnatural base pairs using nanopore sequencing Article Snippet: Tris base (10708976001), 5 M betaine solution (B0300-1VL), 6 N hydrochloric acid (1430071000), GelGreen (SCT124), premixed TBE buffer (10×, 11666703001), potassium chloride (P3911-500G), potassium acetate (P1190-100G), bovine serum albumin (A7030-10G), dimethyl sulfoxide (DMSO; D8418-250ML), magnesium chloride hexahydrate (63068-250 G), TritonTM X-100 (X100-500ML), and sodium chloride (S3014-5KG) were purchased from Sigma-Aldrich (St. Louis, MO). .. Restriction enzymes, Exo III (M0206L), thermolabile Exo I (M0568L), Exo I (M0293L), Exo VIII (truncated; M0545S), NEBNext Ultra TM II End Repair/dA-Tailing Module (E7546S), Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Article Title: In vivo Gene Writing with engineered retrotransposons. Article Snippet: Total DNA was quantified using Qubit 1X HS dsDNA (Thermo Fisher Scientific, Cat# Q33231), and 500 ng of DNA per sample was mechanically sheared to an average of 1,200 bp using a Covaris LE220plus. .. Following a 0.9× AMPure XP SPRI bead (Beckman Coulter, Cat# A63882) cleanup, the fragmented DNA was end-repaired and A-tailed using NEBNext End Prep Master Mix (New England Biolabs, Cat# E7546) and Y-adaptors were ligated using NEBNext Ultra II Ligation Master Mix and Article Title: Functional mapping of IFITM3 resolves its antiviral core and deciphers human influenza susceptibility Article Snippet: Briefly, DNA fragments underwent end repair, 5′ phosphorylation, and dA-tailing in a 15 μL reaction containing enzyme mix and buffer (NEB, Next Ultra II End Repair/dATailing Module, E7546L), incubated sequentially at 20 °C for 30min and 65 °C for 30min. .. Adapter ligation was performed using a 15-fold diluted NEBNext multiplex adapter (NEB, E7335S) in the presence of Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses Article Snippet: .. Libraries were prepared by blunting and dA-tailing the amplicons using the End Prep library kit (NEB; Ref: E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, Ref: E7595) with the adapters obtained from Incubation:Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity Article Snippet: .. Ligation was carried out using the Article Title: Functional mapping of IFITM3 resolves its antiviral core and deciphers human influenza susceptibility Article Snippet: Briefly, DNA fragments underwent end repair, 5′ phosphorylation, and dA-tailing in a 15 μL reaction containing enzyme mix and buffer (NEB, Next Ultra II End Repair/dATailing Module, E7546L), incubated sequentially at 20 °C for 30min and 65 °C for 30min. .. Adapter ligation was performed using a 15-fold diluted NEBNext multiplex adapter (NEB, E7335S) in the presence of Binding Assay:Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Reverse Transcription:Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Isolation:Article Title: TEAD4 Regulates apical domain homeostasis and cell-positioning to maintain the trophectoderm lineage during mouse blastocyst development. Article Snippet: .. 16: 250441 ....................................................................... 9 one-derived isolation buffer lots were pooled (20 μ r the manufacturer’s instructions (PicoPure RNA I cribed [71 ], using the Adapter Ligation:Article Title: Functional mapping of IFITM3 resolves its antiviral core and deciphers human influenza susceptibility Article Snippet: Briefly, DNA fragments underwent end repair, 5′ phosphorylation, and dA-tailing in a 15 μL reaction containing enzyme mix and buffer (NEB, Next Ultra II End Repair/dATailing Module, E7546L), incubated sequentially at 20 °C for 30min and 65 °C for 30min. .. Adapter ligation was performed using a 15-fold diluted NEBNext multiplex adapter (NEB, E7335S) in the presence of Multiplex Assay:Article Title: Functional mapping of IFITM3 resolves its antiviral core and deciphers human influenza susceptibility Article Snippet: Briefly, DNA fragments underwent end repair, 5′ phosphorylation, and dA-tailing in a 15 μL reaction containing enzyme mix and buffer (NEB, Next Ultra II End Repair/dATailing Module, E7546L), incubated sequentially at 20 °C for 30min and 65 °C for 30min. .. Adapter ligation was performed using a 15-fold diluted NEBNext multiplex adapter (NEB, E7335S) in the presence of Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses Article Snippet: .. Libraries were prepared by blunting and dA-tailing the amplicons using the End Prep library kit (NEB; Ref: E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, Ref: E7595) with the adapters obtained from |