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lambda-zapii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda-zapii vector
    Lambda Zapii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda-zapii+vector/pmc05419465-161-11-13
    Average 90 stars, based on 1 article reviews
    lambda-zapii vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cloning:

    Article Title: Cross-bridge kinetics of fast and slow fibres of cat jaw and limb muscles: correlations with myosin subunit composition.
    Article Snippet: Mechanical properties of the jaw-closing muscles of the cat are poorly understood.. These muscles are known to differ in myosin and fibre type compositions from limb muscles.. This work aims to correlate mechanical properties of single fibres in cat jaw and limb muscles with their myosin subunit compositions.

    Article Title: Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene.
    Article Snippet: The Chlamydomonas reinhardtii tla1 (truncated light-harvesting chlorophyll antenna size) mutant was generated upon DNA insertional mutagenesis and shown to speciWcally possess a smaller than wild type (WT) chlorophyll antenna size in both photosystems.. Molecular and genetic analysis revealed that the exogenous plasmid DNA was inserted at the end of the 5 UTR and just prior to the ATG start codon of a hitherto unknown nuclear gene (termed Tla1), which encodes a protein of 213 amino acids.. The Tla1 gene in the mutant is transcribed with a new 5 UTR sequence, derived from the 3 end of the transforming plasmid.

    Construct:

    Article Title: Cross-bridge kinetics of fast and slow fibres of cat jaw and limb muscles: correlations with myosin subunit composition.
    Article Snippet: Mechanical properties of the jaw-closing muscles of the cat are poorly understood.. These muscles are known to differ in myosin and fibre type compositions from limb muscles.. This work aims to correlate mechanical properties of single fibres in cat jaw and limb muscles with their myosin subunit compositions.

    Article Title: The cloning of one putative octopamine receptor and two putative serotonin receptors from the tobacco hawkmoth, Manduca sexta
    Article Snippet: .. All libraries were constructed by generating oligo-dT-primed double-stranded cDNA and then packaging the cDNA into the Lambda ZapII vector (Stratagene, La Jolla, CA). ..

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    other:

    Article Title: A site-specific insertion sequence in flax genotrophs induced by environment.
    Article Snippet: • A single-copy 5.7 kilobase (kb) DNA fragment, termed Linum Insertion Sequence 1 (LIS-1), has been identified and characterized.. This is one of the DNA changes associated with the environmentally induced heritable changes resulting in stable lines termed genotrophs in flax ( Linum usitatissimum ).. • The insertion sequence and its insertion site have been cloned from genomic libraries and sequenced.

    Article Title: Structure and origin of the White Cap locus and its role in evolution of grain color in maize
    Article Snippet: The fraction enriched in 6.5 kb fragments was purified and ligated to lambda-ZAPII vector (Stratagene).

    Article Title: Structure and Origin of the White Cap Locus and Its Role in Evolution of Grain Color in Maize
    Article Snippet: The fraction enriched in 6.5-kb fragments was purified and ligated to lambda-ZAPII vector (Stratagene, La Jolla, CA).

    Plasmid Preparation:

    Article Title: The cloning of one putative octopamine receptor and two putative serotonin receptors from the tobacco hawkmoth, Manduca sexta
    Article Snippet: .. All libraries were constructed by generating oligo-dT-primed double-stranded cDNA and then packaging the cDNA into the Lambda ZapII vector (Stratagene, La Jolla, CA). ..

    Article Title: Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene.
    Article Snippet: The Chlamydomonas reinhardtii tla1 (truncated light-harvesting chlorophyll antenna size) mutant was generated upon DNA insertional mutagenesis and shown to speciWcally possess a smaller than wild type (WT) chlorophyll antenna size in both photosystems.. Molecular and genetic analysis revealed that the exogenous plasmid DNA was inserted at the end of the 5 UTR and just prior to the ATG start codon of a hitherto unknown nuclear gene (termed Tla1), which encodes a protein of 213 amino acids.. The Tla1 gene in the mutant is transcribed with a new 5 UTR sequence, derived from the 3 end of the transforming plasmid.

    cDNA Library Assay:

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    Article Title: S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding.
    Article Snippet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) influences cytotoxicity, translocating to the nucleus during apoptosis.. Here we report a signalling pathway in which nitric oxide (NO) generation that follows apoptotic stimulation elicits S-nitrosylation of GAPDH, which triggers binding to Siah1 (an E3 ubiquitin ligase), nuclear translocation and apoptosis.. S-nitrosylation of GAPDH augments its binding to Siah1, whose nuclear localization signal mediates translocation of GAPDH.

    Article Title: Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene.
    Article Snippet: The Chlamydomonas reinhardtii tla1 (truncated light-harvesting chlorophyll antenna size) mutant was generated upon DNA insertional mutagenesis and shown to speciWcally possess a smaller than wild type (WT) chlorophyll antenna size in both photosystems.. Molecular and genetic analysis revealed that the exogenous plasmid DNA was inserted at the end of the 5 UTR and just prior to the ATG start codon of a hitherto unknown nuclear gene (termed Tla1), which encodes a protein of 213 amino acids.. The Tla1 gene in the mutant is transcribed with a new 5 UTR sequence, derived from the 3 end of the transforming plasmid.

    Generated:

    Article Title: Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene.
    Article Snippet: The Chlamydomonas reinhardtii tla1 (truncated light-harvesting chlorophyll antenna size) mutant was generated upon DNA insertional mutagenesis and shown to speciWcally possess a smaller than wild type (WT) chlorophyll antenna size in both photosystems.. Molecular and genetic analysis revealed that the exogenous plasmid DNA was inserted at the end of the 5 UTR and just prior to the ATG start codon of a hitherto unknown nuclear gene (termed Tla1), which encodes a protein of 213 amino acids.. The Tla1 gene in the mutant is transcribed with a new 5 UTR sequence, derived from the 3 end of the transforming plasmid.



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    Agilent technologies cdna library lambda zapii vector
    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal <t>cDNA</t> <t>library.</t> The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
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    Image Search Results


    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Journal:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation

    doi: 10.1016/j.neuron.2009.05.024

    Figure Lengend Snippet: (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Article Snippet: An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim).

    Techniques: Construct, cDNA Library Assay