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λ zapii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies λ zapii vector
    λ Zapii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda-zapii+vector/pmc02670833-194-19-22
    Average 90 stars, based on 1 article reviews
    λ zapii vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: Identification of glutamic acid decarboxylase gene and distribution of GABAergic nervous system in the planarian Dugesia japonica.
    Article Snippet: bstract—The planarian Dugesia japonica has a relatively ell-organized CNS that includes the brain and the ventral erve cords, and also has high regenerative capacity derived rom pluripotent stem cells present in the mesenchymal pace throughout the body.. Glutamic acid decarboxylase GAD) is the enzyme that converts glutamic acid into GABA, major inhibitory neurotransmitter.. In this study, we first dentified a full-length GAD gene (DjGAD, D. japonica gluamic acid decarboxylase) in the planarian D. japonica. hole-mount in situ hybridization revealed that a few cells xpressed DjGAD mRNA, and these cells were located in oth the head and pharynx regions.

    Article Title: Cloning and pattern of expression of the shiro-uo vasa gene during embryogenesis and its roles in PGC development.
    Article Snippet: For 5’-RACE, mRNA was extracted from shiro-uo ovaries and firststrand cDNA was synthesized using a SMART cDNA Library Construction Kit (Clontech). .. For 3’-RACE, a shiro-uo embryonic cDNA library in the λ zapII vector (Stratagene) was used. ..

    Article Title: Ethylene modulates transcription of the phosphoenolpyruvate carboxykinase gene in grapefruit flavedo
    Article Snippet: Ethylene modulates transcription of the phosphoenolpyruvate carboxykinase gene in grapefruit flavedo D.P.. Maul, W. Mckendree, M. Bausher & T.G.. Mccollum To cite this article: D.P.

    Article Title: USP19 is a ubiquitin-specific protease regulated in rat skeletal muscle during catabolic states.
    Article Snippet: The amplified DNA fragments were subcloned into the pGEM-T vector (Promega) and sequenced with the dideoxy chain termination method and T7 polymerase (Pharmacia). .. Because the predicted protein sequence showed marked similarity to other motifs conserved in UBPs (37), the PCR-amplified DNA fragment from clone 1 was labeled with 32P and used as a probe to screen a rat testis cDNA library in the zapII vector (Stratagene). ..

    Article Title: Abiotic-stress induces demethylation and transcriptional activation of a gene encoding a glycerophosphodiesterase-like protein in tobacco plants.
    Article Snippet: To examine the relationship between gene expression and DNA methylation, transcriptionally activated genes were screened in hypomethylated transgenic tobacco plants expressing an anti-DNA methyltransferase sequence.. Among 16 genes initially identiWed, one clone was found to encode a glycerophosphodiesterase-like protein (NtGPDL), earlier reported to be responsive to aluminium stress.. When detached leaves from wild type tobacco plants were treated with aluminium, NtGPDL transcripts were induced within 6 h, and corresponding genomic loci were demethylated at CCGG sites within 1 h. Direct bisulWte methylation mapping revealed that CG sites in coding regions were selectively demethylated, and that promoter regions were totally unmethylated regardless of the stress.

    Construct:

    Article Title: Identification of glutamic acid decarboxylase gene and distribution of GABAergic nervous system in the planarian Dugesia japonica.
    Article Snippet: bstract—The planarian Dugesia japonica has a relatively ell-organized CNS that includes the brain and the ventral erve cords, and also has high regenerative capacity derived rom pluripotent stem cells present in the mesenchymal pace throughout the body.. Glutamic acid decarboxylase GAD) is the enzyme that converts glutamic acid into GABA, major inhibitory neurotransmitter.. In this study, we first dentified a full-length GAD gene (DjGAD, D. japonica gluamic acid decarboxylase) in the planarian D. japonica. hole-mount in situ hybridization revealed that a few cells xpressed DjGAD mRNA, and these cells were located in oth the head and pharynx regions.

    Article Title: A new genomic resource dedicated to wood formation in Eucalyptus
    Article Snippet: .. A library of directionally-cloned cDNAs prepared from the developing secondary xylem tissue of Eucalyptus gunnii was constructed in the λ ZapII vector (Stratagene, Amsterdam, The Netherlands) [ ]. ..

    Plasmid Preparation:

    Article Title: Cloning and pattern of expression of the shiro-uo vasa gene during embryogenesis and its roles in PGC development.
    Article Snippet: For 5’-RACE, mRNA was extracted from shiro-uo ovaries and firststrand cDNA was synthesized using a SMART cDNA Library Construction Kit (Clontech). .. For 3’-RACE, a shiro-uo embryonic cDNA library in the λ zapII vector (Stratagene) was used. ..

    Article Title: USP19 is a ubiquitin-specific protease regulated in rat skeletal muscle during catabolic states.
    Article Snippet: The amplified DNA fragments were subcloned into the pGEM-T vector (Promega) and sequenced with the dideoxy chain termination method and T7 polymerase (Pharmacia). .. Because the predicted protein sequence showed marked similarity to other motifs conserved in UBPs (37), the PCR-amplified DNA fragment from clone 1 was labeled with 32P and used as a probe to screen a rat testis cDNA library in the zapII vector (Stratagene). ..

    Article Title: Suppression of anchorage-independent growth by expression of the ataxia-telangiectasia group D complementing gene, ATDC.
    Article Snippet: The ataxia-telangiectasia group D complementing gene, ATDC, is located at 11q23, where loss of heterozygosity (LOH) is frequently observed in many kinds of cancers including breast cancer.. Underexpression of ATDC in breast and prostate cancer has been reported using serial analysis of gene expression (SAGE) and DNA microarray analysis.. We previously reported that SV-40-transformation down-regulates the expression of ATDC.

    Article Title: In Xenopus Egg Extracts, DNA Replication Initiates Preferentially at or near Asymmetric AT Sequences
    Article Snippet: .. The DNA fragment containing the TATA box of the Xenopus c-myc gene adjacent to five binding sites for the transcription factor Gal4-VP16 was inserted into the zapII vector (Stratagene) derived from wt DNA. ..

    Chromatography:

    Article Title: Ethylene modulates transcription of the phosphoenolpyruvate carboxykinase gene in grapefruit flavedo
    Article Snippet: Ethylene modulates transcription of the phosphoenolpyruvate carboxykinase gene in grapefruit flavedo D.P.. Maul, W. Mckendree, M. Bausher & T.G.. Mccollum To cite this article: D.P.

    Sequencing:

    Article Title: USP19 is a ubiquitin-specific protease regulated in rat skeletal muscle during catabolic states.
    Article Snippet: The amplified DNA fragments were subcloned into the pGEM-T vector (Promega) and sequenced with the dideoxy chain termination method and T7 polymerase (Pharmacia). .. Because the predicted protein sequence showed marked similarity to other motifs conserved in UBPs (37), the PCR-amplified DNA fragment from clone 1 was labeled with 32P and used as a probe to screen a rat testis cDNA library in the zapII vector (Stratagene). ..

    Polymerase Chain Reaction:

    Article Title: USP19 is a ubiquitin-specific protease regulated in rat skeletal muscle during catabolic states.
    Article Snippet: The amplified DNA fragments were subcloned into the pGEM-T vector (Promega) and sequenced with the dideoxy chain termination method and T7 polymerase (Pharmacia). .. Because the predicted protein sequence showed marked similarity to other motifs conserved in UBPs (37), the PCR-amplified DNA fragment from clone 1 was labeled with 32P and used as a probe to screen a rat testis cDNA library in the zapII vector (Stratagene). ..

    Labeling:

    Article Title: USP19 is a ubiquitin-specific protease regulated in rat skeletal muscle during catabolic states.
    Article Snippet: The amplified DNA fragments were subcloned into the pGEM-T vector (Promega) and sequenced with the dideoxy chain termination method and T7 polymerase (Pharmacia). .. Because the predicted protein sequence showed marked similarity to other motifs conserved in UBPs (37), the PCR-amplified DNA fragment from clone 1 was labeled with 32P and used as a probe to screen a rat testis cDNA library in the zapII vector (Stratagene). ..

    Isolation:

    Article Title: Suppression of anchorage-independent growth by expression of the ataxia-telangiectasia group D complementing gene, ATDC.
    Article Snippet: The ataxia-telangiectasia group D complementing gene, ATDC, is located at 11q23, where loss of heterozygosity (LOH) is frequently observed in many kinds of cancers including breast cancer.. Underexpression of ATDC in breast and prostate cancer has been reported using serial analysis of gene expression (SAGE) and DNA microarray analysis.. We previously reported that SV-40-transformation down-regulates the expression of ATDC.

    Article Title: Abiotic-stress induces demethylation and transcriptional activation of a gene encoding a glycerophosphodiesterase-like protein in tobacco plants.
    Article Snippet: To examine the relationship between gene expression and DNA methylation, transcriptionally activated genes were screened in hypomethylated transgenic tobacco plants expressing an anti-DNA methyltransferase sequence.. Among 16 genes initially identiWed, one clone was found to encode a glycerophosphodiesterase-like protein (NtGPDL), earlier reported to be responsive to aluminium stress.. When detached leaves from wild type tobacco plants were treated with aluminium, NtGPDL transcripts were induced within 6 h, and corresponding genomic loci were demethylated at CCGG sites within 1 h. Direct bisulWte methylation mapping revealed that CG sites in coding regions were selectively demethylated, and that promoter regions were totally unmethylated regardless of the stress.

    Binding Assay:

    Article Title: In Xenopus Egg Extracts, DNA Replication Initiates Preferentially at or near Asymmetric AT Sequences
    Article Snippet: .. The DNA fragment containing the TATA box of the Xenopus c-myc gene adjacent to five binding sites for the transcription factor Gal4-VP16 was inserted into the zapII vector (Stratagene) derived from wt DNA. ..

    Derivative Assay:

    Article Title: In Xenopus Egg Extracts, DNA Replication Initiates Preferentially at or near Asymmetric AT Sequences
    Article Snippet: .. The DNA fragment containing the TATA box of the Xenopus c-myc gene adjacent to five binding sites for the transcription factor Gal4-VP16 was inserted into the zapII vector (Stratagene) derived from wt DNA. ..



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    Agilent technologies cdna library lambda zapii vector
    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal <t>cDNA</t> <t>library.</t> The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
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    Image Search Results


    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Journal:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation

    doi: 10.1016/j.neuron.2009.05.024

    Figure Lengend Snippet: (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Article Snippet: An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim).

    Techniques: Construct, cDNA Library Assay