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cdna library lambda zapii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies cdna library lambda zapii vector
    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal <t>cDNA</t> <t>library.</t> The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
    Cdna Library Lambda Zapii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda-zapii+vector/pmc02758064-300-2-10
    Average 90 stars, based on 1 article reviews
    cdna library lambda zapii vector - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation"

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation

    Journal:

    doi: 10.1016/j.neuron.2009.05.024

    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
    Figure Legend Snippet: (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Techniques Used: Construct, cDNA Library Assay

    Related Articles

    Isolation:

    Article Title: Identification and Characterization of a Novel Serine-Arginine-Rich Splicing Regulatory Protein
    Article Snippet: .. A clone containing a partial open reading frame (ORF) was isolated during the initial screen and then used to probe a rat brain cDNA library (Stratagene). ..

    Article Title: Protein Phosphatase-1 Regulation in the Induction of Long-Term Potentiation: Heterogeneous Molecular Mechanisms
    Article Snippet: .. Murine I-1 clones were isolated from a 129SvJ strain genomic library (Stratagene, La Jolla, CA) using a rat cDNA (Elbrecht et al., 1990) as a probe. ..

    Article Title: Protein Phosphatase-1 Regulation in the Induction of Long-Term Potentiation: Heterogeneous Molecular Mechanisms
    Article Snippet: .. Murine I-1 clones were isolated from a 129SvJ strain genomic library (Stratagene, La Jolla, CA) using a rat cDNA ( Elbrecht et al., 1990 ) as a probe. ..

    cDNA Library Assay:

    Article Title: Identification and Characterization of a Novel Serine-Arginine-Rich Splicing Regulatory Protein
    Article Snippet: .. A clone containing a partial open reading frame (ORF) was isolated during the initial screen and then used to probe a rat brain cDNA library (Stratagene). ..

    Article Title: PAPIN
    Article Snippet: .. Yeast Two-hybrid Screening and cDNA Screening—Yeast two-hybrid screening was performed using a rat brain cDNA library as described previously (16). cDNA library screenings were performed by plaque hybridization using rat brain cDNA libraries (Stratagene) (16). .. Construction of Expression Vectors—Various expression vectors were constructed by conventional molecular biology techniques and the polymerase chain reaction method using pCMV Myc, pClneo Myc, and pGex5X-3 (Amersham Pharmacia Biotech). pClneo Myc d-catenin was described previously (15).

    Article Title: Striatum- and testis-specific phosphodiesterase PDE10A isolation and characterization of a rat PDE10A.
    Article Snippet: [a-32P]dCTP and Hybond-N+ nylon membrane were from Amersham Pharmacia Biotech. .. The plasmid pBluescript II SK(+) and the rat brain cDNA library were from Stratagene. .. The TA-cloning vector pGEM-T easy was from Promega.

    Article Title: The Small GTP-Binding Protein TC10 Promotes Nerve Elongation in Neuronal Cells, and Its Expression Is induced during Nerve Regeneration in Rats
    Article Snippet: .. A rat brain cDNA library (Lambda ZAPII library, Stratagene, La Jolla, CA) was screened with a subcloned 739bp DNA fragment by means of plaque hybridization. ..

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    Two Hybrid Screening:

    Article Title: PAPIN
    Article Snippet: .. Yeast Two-hybrid Screening and cDNA Screening—Yeast two-hybrid screening was performed using a rat brain cDNA library as described previously (16). cDNA library screenings were performed by plaque hybridization using rat brain cDNA libraries (Stratagene) (16). .. Construction of Expression Vectors—Various expression vectors were constructed by conventional molecular biology techniques and the polymerase chain reaction method using pCMV Myc, pClneo Myc, and pGex5X-3 (Amersham Pharmacia Biotech). pClneo Myc d-catenin was described previously (15).

    Hybridization:

    Article Title: PAPIN
    Article Snippet: .. Yeast Two-hybrid Screening and cDNA Screening—Yeast two-hybrid screening was performed using a rat brain cDNA library as described previously (16). cDNA library screenings were performed by plaque hybridization using rat brain cDNA libraries (Stratagene) (16). .. Construction of Expression Vectors—Various expression vectors were constructed by conventional molecular biology techniques and the polymerase chain reaction method using pCMV Myc, pClneo Myc, and pGex5X-3 (Amersham Pharmacia Biotech). pClneo Myc d-catenin was described previously (15).

    Article Title: The Small GTP-Binding Protein TC10 Promotes Nerve Elongation in Neuronal Cells, and Its Expression Is induced during Nerve Regeneration in Rats
    Article Snippet: .. A rat brain cDNA library (Lambda ZAPII library, Stratagene, La Jolla, CA) was screened with a subcloned 739bp DNA fragment by means of plaque hybridization. ..

    Plasmid Preparation:

    Article Title: Striatum- and testis-specific phosphodiesterase PDE10A isolation and characterization of a rat PDE10A.
    Article Snippet: [a-32P]dCTP and Hybond-N+ nylon membrane were from Amersham Pharmacia Biotech. .. The plasmid pBluescript II SK(+) and the rat brain cDNA library were from Stratagene. .. The TA-cloning vector pGEM-T easy was from Promega.

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    Labeling:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    DNA Labeling:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    Clone Assay:

    Article Title: Protein Phosphatase-1 Regulation in the Induction of Long-Term Potentiation: Heterogeneous Molecular Mechanisms
    Article Snippet: .. Murine I-1 clones were isolated from a 129SvJ strain genomic library (Stratagene, La Jolla, CA) using a rat cDNA (Elbrecht et al., 1990) as a probe. ..

    Article Title: Protein Phosphatase-1 Regulation in the Induction of Long-Term Potentiation: Heterogeneous Molecular Mechanisms
    Article Snippet: .. Murine I-1 clones were isolated from a 129SvJ strain genomic library (Stratagene, La Jolla, CA) using a rat cDNA ( Elbrecht et al., 1990 ) as a probe. ..



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    Agilent technologies cdna library lambda zapii vector
    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal <t>cDNA</t> <t>library.</t> The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
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    Image Search Results


    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Journal:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation

    doi: 10.1016/j.neuron.2009.05.024

    Figure Lengend Snippet: (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Article Snippet: An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim).

    Techniques: Construct, cDNA Library Assay