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commercial immunostaining blocking solution  (Beyotime)


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    Beyotime commercial immunostaining blocking solution
    Commercial Immunostaining Blocking Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunostaining+blocking+solution/quickblocktm+blocking+buffer/pmc12269989-357-32-35
    Average 90 stars, based on 1 article reviews
    commercial immunostaining blocking solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Exosomal miR-30a-5p promoted intrahepatic cholangiocarcinoma progression by increasing angiogenesis and vascular permeability dependent on PDCD10
    Article Snippet: .. Then, the gel was transferred to nitrocellulose membranes, blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 15 minutes, incubated with primary antibody overnight at 4◦C and with secondary antibody at room temperature for 2h. .. The exposed Proteins images were visualized with ECL Plus (EMD Millipore, Billarica, MA, USA).

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Article Title: Noninvasive method for achieving the regeneration of damaged nerves via ultrasonic nasal drops.
    Article Snippet: .. The membrane was first blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 30 min, followed by overnight incubation at 4 ◦C with primary antibodies. .. After being washed three times with Western Wash Buffer (Beyotime, China), the membrane was incubated with a secondary antibody—either goat antimouse (1:400, Beyotime, China) or goat anti-rabbit (1:400, Beyotime, China)—for 1.5 h at room temperature.

    Article Title: The lateral habenula contributes to regulation of body temperature
    Article Snippet: It was cut into 20-μm slices using a frozen sectioning machine (LEICA CM1950, Nussloch, Germany). .. The brain tissue sections were washed with PBS three times at room temperature for 10 min each and then incubated in 0.3% Triton X-100 for 30 min. After blocking with a commercial immunostaining blocking solution (Beyotime: P0260) for 1 h, the sections were incubated overnight at 4°C with rat anti-c-Fos primary antibody (Synaptic Systems, 226017, 1:1000), rabbit anti-c-Fos primary antibody (Abcam, ab190289, 1:1000), rabbit anti-Glutamate primary antibody (Sigma, G6642, 1: 200), mouse anti-GABA primary antibody (Sigma, A0310, 1: 200), and rabbit anti-NeuN (CST, 24307s, 1:1000). .. Next day, the slices were incubated for 2 h at room temperature with donkey anti-rabbit IgG secondary antibody (Invitrogen, A21206, H + L, Alexa FluorTM488), goat anti-mouse IgG secondary antibody (Abcam, ab150113, H + L, Alexa FluorTM488), donkey anti-rat IgG secondary antibody (Invitrogen, A11077, H + L, Alexa FluorTM568) and goat anti-rat IgG secondary antibody (Invitrogen, A21247, H + L, Alexa FluorTM647).

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

    Incubation:

    Article Title: Exosomal miR-30a-5p promoted intrahepatic cholangiocarcinoma progression by increasing angiogenesis and vascular permeability dependent on PDCD10
    Article Snippet: .. Then, the gel was transferred to nitrocellulose membranes, blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 15 minutes, incubated with primary antibody overnight at 4◦C and with secondary antibody at room temperature for 2h. .. The exposed Proteins images were visualized with ECL Plus (EMD Millipore, Billarica, MA, USA).

    Article Title: KLF5-regulated ZDHHC8 reduces chemosensitivity in high-grade serous ovarian cancer through β-catenin signaling
    Article Snippet: Equal amounts of protein (10 μg per sample) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 12% gel and transferred to PVDF membranes (Millipore, Germany). .. Membranes were blocked with 5% nonfat milk in TBST (Beyotime, Shanghai, China, Cat# P0231) for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against ZDHHC8 (1:1000, Cat# 27179-1-AP), KLF5 (1:1000, Cat# 21017-1-AP), β-catenin (1:5000, Cat# 51067-2-AP), β-actin (1:5000, Cat# 81115-1-RR), and H3 (1:2000, Cat# 17168-1-AP) (Proteintech, USA). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000, ZSGB-Bio, Beijing, China, Cat# ZB-2301) for 2 h at room temperature.

    Article Title: Construction and evaluation of recombinant Lactobacillus plantarum expressing Micropterus salmoides hepcidin.
    Article Snippet: Proteins were transferred to a PVDF membrane (0.22 um; Beyotime, FFP70; China) for 28 min. .. The membrane was blocked with sealing fluid TBST (Beyotime, P0231; China) for 2 h at 37 ◦ C, then incubated with a primary 6 × His-Tag antibody (1:2,000 dilution; Thermo Fisher, HRP66,005; China) overnight at 4 ◦ C. After three 10-min TBST washes with agitation, the membrane was probed with a HRP-conjugated goat anti-rabbit IgG secondary antibody (1:500 dilution; Beyotime, P09478; China) for 1 h at 37 ◦ C. Following three 15-min TBST washes, signals were detected using an ECL chemiluminescence kit (Beyotime, P0018; China). ..

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Article Title: Noninvasive method for achieving the regeneration of damaged nerves via ultrasonic nasal drops.
    Article Snippet: .. The membrane was first blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 30 min, followed by overnight incubation at 4 ◦C with primary antibodies. .. After being washed three times with Western Wash Buffer (Beyotime, China), the membrane was incubated with a secondary antibody—either goat antimouse (1:400, Beyotime, China) or goat anti-rabbit (1:400, Beyotime, China)—for 1.5 h at room temperature.

    Article Title: The lateral habenula contributes to regulation of body temperature
    Article Snippet: It was cut into 20-μm slices using a frozen sectioning machine (LEICA CM1950, Nussloch, Germany). .. The brain tissue sections were washed with PBS three times at room temperature for 10 min each and then incubated in 0.3% Triton X-100 for 30 min. After blocking with a commercial immunostaining blocking solution (Beyotime: P0260) for 1 h, the sections were incubated overnight at 4°C with rat anti-c-Fos primary antibody (Synaptic Systems, 226017, 1:1000), rabbit anti-c-Fos primary antibody (Abcam, ab190289, 1:1000), rabbit anti-Glutamate primary antibody (Sigma, G6642, 1: 200), mouse anti-GABA primary antibody (Sigma, A0310, 1: 200), and rabbit anti-NeuN (CST, 24307s, 1:1000). .. Next day, the slices were incubated for 2 h at room temperature with donkey anti-rabbit IgG secondary antibody (Invitrogen, A21206, H + L, Alexa FluorTM488), goat anti-mouse IgG secondary antibody (Abcam, ab150113, H + L, Alexa FluorTM488), donkey anti-rat IgG secondary antibody (Invitrogen, A11077, H + L, Alexa FluorTM568) and goat anti-rat IgG secondary antibody (Invitrogen, A21247, H + L, Alexa FluorTM647).

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

    Membrane:

    Article Title: Construction and evaluation of recombinant Lactobacillus plantarum expressing Micropterus salmoides hepcidin.
    Article Snippet: Proteins were transferred to a PVDF membrane (0.22 um; Beyotime, FFP70; China) for 28 min. .. The membrane was blocked with sealing fluid TBST (Beyotime, P0231; China) for 2 h at 37 ◦ C, then incubated with a primary 6 × His-Tag antibody (1:2,000 dilution; Thermo Fisher, HRP66,005; China) overnight at 4 ◦ C. After three 10-min TBST washes with agitation, the membrane was probed with a HRP-conjugated goat anti-rabbit IgG secondary antibody (1:500 dilution; Beyotime, P09478; China) for 1 h at 37 ◦ C. Following three 15-min TBST washes, signals were detected using an ECL chemiluminescence kit (Beyotime, P0018; China). ..

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Article Title: Noninvasive method for achieving the regeneration of damaged nerves via ultrasonic nasal drops.
    Article Snippet: .. The membrane was first blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 30 min, followed by overnight incubation at 4 ◦C with primary antibodies. .. After being washed three times with Western Wash Buffer (Beyotime, China), the membrane was incubated with a secondary antibody—either goat antimouse (1:400, Beyotime, China) or goat anti-rabbit (1:400, Beyotime, China)—for 1.5 h at room temperature.

    other:

    Article Title: Rutin ameliorates imiquimod-induced psoriasis-like skin lesions by inhibiting oxidative stress injury and the inflammatory response in mice via the Keap1/Nrf2 signaling pathway.
    Article Snippet: The membranes were blocked by blocking buffer (Beyotime, Cat# P0252, Shanghai, China) for 10 min, and then incubated with the following primary antibodies: Nrf2 (1:1000), Keap1 (1:2000), and β-actin (1:2000) at 4 °C overnight.

    Western Blot:

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Binding Assay:

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Immunostaining:

    Article Title: The lateral habenula contributes to regulation of body temperature
    Article Snippet: It was cut into 20-μm slices using a frozen sectioning machine (LEICA CM1950, Nussloch, Germany). .. The brain tissue sections were washed with PBS three times at room temperature for 10 min each and then incubated in 0.3% Triton X-100 for 30 min. After blocking with a commercial immunostaining blocking solution (Beyotime: P0260) for 1 h, the sections were incubated overnight at 4°C with rat anti-c-Fos primary antibody (Synaptic Systems, 226017, 1:1000), rabbit anti-c-Fos primary antibody (Abcam, ab190289, 1:1000), rabbit anti-Glutamate primary antibody (Sigma, G6642, 1: 200), mouse anti-GABA primary antibody (Sigma, A0310, 1: 200), and rabbit anti-NeuN (CST, 24307s, 1:1000). .. Next day, the slices were incubated for 2 h at room temperature with donkey anti-rabbit IgG secondary antibody (Invitrogen, A21206, H + L, Alexa FluorTM488), goat anti-mouse IgG secondary antibody (Abcam, ab150113, H + L, Alexa FluorTM488), donkey anti-rat IgG secondary antibody (Invitrogen, A11077, H + L, Alexa FluorTM568) and goat anti-rat IgG secondary antibody (Invitrogen, A21247, H + L, Alexa FluorTM647).

    Immunofluorescence:

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

    Staining:

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.



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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows <t>IHC</t> images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.
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    Image Search Results


    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Staining

    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Journal: Materials Today Bio

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis

    doi: 10.1016/j.mtbio.2025.102009

    Figure Lengend Snippet: The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Article Snippet: Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China).

    Techniques: Knockdown, Expressing