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immunostaining blocking solution  (Beyotime)


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    Beyotime immunostaining blocking solution
    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows <t>IHC</t> images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.
    Immunostaining Blocking Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunostaining+blocking+solution/immunol+staining+blocking+buffer/pmc12264616-142-55-58
    Average 90 stars, based on 1 article reviews
    immunostaining blocking solution - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis"

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102009

    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.
    Figure Legend Snippet: The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Techniques Used: Knockdown, Expressing

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    Incubation:

    Article Title: Zinc ions trigger PANoptosis-like cell death in magnetic hyperthermia therapy of magnesium based implant for hepatocellular carcinoma.
    Article Snippet: According to Global Cancer Statistics 2022, primary liver cancer is the sixth of the top 10 most diagnosed cancers and the third leading cause of cancer death, and more than 75% of the primary liver cancer cases are hepatocellular carcinoma (HCC) [1].. Worsely, the incidence of HCC is increasing year by year worldwide.. In China, there are more than 50% of the world’s newly diagnosed liver cancer cases and deaths, and even over 80% of liver cancer cases are HCC [2].

    Article Title: Melittin inhibits MPP + -induced HT22 cell death by suppressing Bax activation and improving mitochondrial function
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min and permeabilised with 0.1% Triton X-100 for 30 min. After being washed with PBS and blocked with immunostaining blocking solution (Beyotime Biotechnology), cells were incubated with anti-AIF antibody (1:200, CST, #5318) overnight at 4°C and then incubated with YF 594 goat anti-rabbit IgG (1:200, UElandy) for 2 h, followed by incubation with DAPI (UElandy) for 15 min. .. Finally, cell images were acquired using a confocal laser scanning microscope (Leica).

    Article Title: Disruption of intracellular calcium homeostasis drives graphene quantum dots-induced inflammatory response in liver macrophages
    Article Snippet: Graphene quantum dots (GQDs), with photoluminescent properties, high stability, and excellent biocompatibility, hold tremendous potential in biomedicine.. It is urgent to evaluate their safety and potential health risks to promote their clinical application.. Kupffer cells (KCs), as primary immune cells encountered by foreign substances entering the liver and integral to liver immunity, have yet to be systematically studied for toxicity responses to different GQDs.

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis
    Article Snippet: Various treatment conditions were applied for a duration of 48 h. Post-treatment, the cells were gently rinsed twice with PBS, followed by fixation using Beyotime (China) for 10 min. .. Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China). .. Following washing with PBS, the Cytochrome c oxidase IV (COX IV, CST, USA) -specific primary antibody was applied and incubated overnight.

    Article Title: GPCR-MAPK signaling pathways underpin fitness trade-offs in whitefly
    Article Snippet: .. After washing with PBST for three times, the213 specimens were blocked in Immunol Staining Blocking Buffer Kit (Beyotime, China) at214 4 °C for 4 h, followed by incubation with the primary antibodies against Ex (1:400), Va215 (1:600), Bg (1:400) and Vg (1:400) proteins lasted at 4 °C overnight. .. The samples were216 then incubated against the secondary antibody Alexa Fluor® 555 goat anti-rabbit lgG217 (1:500) (Abcam) for 2 h at room temperature in a black box, and then washed with PBST218 five times.

    Article Title: The Rheb-mTORC1 Coordinates Cell Cycle Progression and Endoreplication in Bombyx mori
    Article Snippet: And the cells were incubated in a cell culture incubator at 27 ◦C for 2–4 h. The cells were then fixed in 4% paraformaldehyde at room temperature for 15–30 min and incubated with Immunol Staining Wash Buffer (Beyotime, Shanghai, China) for 15 min at room temperature. .. After washing with Immunol Staining Blocking Buffer (Beyotime, Shanghai, China) 3 times, the Click reaction solution was added to each well, and incubated for 30 min in the dark. .. Then the cells were stained with 200 μL of DAPI (Beyotime, Shanghai, China) for 10 min, and were observed by fluorescence microscope (Olympus, Tokyo, Japan).

    Staining:

    Article Title: Zinc ions trigger PANoptosis-like cell death in magnetic hyperthermia therapy of magnesium based implant for hepatocellular carcinoma.
    Article Snippet: According to Global Cancer Statistics 2022, primary liver cancer is the sixth of the top 10 most diagnosed cancers and the third leading cause of cancer death, and more than 75% of the primary liver cancer cases are hepatocellular carcinoma (HCC) [1].. Worsely, the incidence of HCC is increasing year by year worldwide.. In China, there are more than 50% of the world’s newly diagnosed liver cancer cases and deaths, and even over 80% of liver cancer cases are HCC [2].

    Article Title: Disruption of intracellular calcium homeostasis drives graphene quantum dots-induced inflammatory response in liver macrophages
    Article Snippet: Graphene quantum dots (GQDs), with photoluminescent properties, high stability, and excellent biocompatibility, hold tremendous potential in biomedicine.. It is urgent to evaluate their safety and potential health risks to promote their clinical application.. Kupffer cells (KCs), as primary immune cells encountered by foreign substances entering the liver and integral to liver immunity, have yet to be systematically studied for toxicity responses to different GQDs.

    Article Title: GPCR-MAPK signaling pathways underpin fitness trade-offs in whitefly
    Article Snippet: .. After washing with PBST for three times, the213 specimens were blocked in Immunol Staining Blocking Buffer Kit (Beyotime, China) at214 4 °C for 4 h, followed by incubation with the primary antibodies against Ex (1:400), Va215 (1:600), Bg (1:400) and Vg (1:400) proteins lasted at 4 °C overnight. .. The samples were216 then incubated against the secondary antibody Alexa Fluor® 555 goat anti-rabbit lgG217 (1:500) (Abcam) for 2 h at room temperature in a black box, and then washed with PBST218 five times.

    Article Title: The Rheb-mTORC1 Coordinates Cell Cycle Progression and Endoreplication in Bombyx mori
    Article Snippet: And the cells were incubated in a cell culture incubator at 27 ◦C for 2–4 h. The cells were then fixed in 4% paraformaldehyde at room temperature for 15–30 min and incubated with Immunol Staining Wash Buffer (Beyotime, Shanghai, China) for 15 min at room temperature. .. After washing with Immunol Staining Blocking Buffer (Beyotime, Shanghai, China) 3 times, the Click reaction solution was added to each well, and incubated for 30 min in the dark. .. Then the cells were stained with 200 μL of DAPI (Beyotime, Shanghai, China) for 10 min, and were observed by fluorescence microscope (Olympus, Tokyo, Japan).

    Blocking Assay:

    Article Title: Zinc ions trigger PANoptosis-like cell death in magnetic hyperthermia therapy of magnesium based implant for hepatocellular carcinoma.
    Article Snippet: According to Global Cancer Statistics 2022, primary liver cancer is the sixth of the top 10 most diagnosed cancers and the third leading cause of cancer death, and more than 75% of the primary liver cancer cases are hepatocellular carcinoma (HCC) [1].. Worsely, the incidence of HCC is increasing year by year worldwide.. In China, there are more than 50% of the world’s newly diagnosed liver cancer cases and deaths, and even over 80% of liver cancer cases are HCC [2].

    Article Title: Melittin inhibits MPP + -induced HT22 cell death by suppressing Bax activation and improving mitochondrial function
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min and permeabilised with 0.1% Triton X-100 for 30 min. After being washed with PBS and blocked with immunostaining blocking solution (Beyotime Biotechnology), cells were incubated with anti-AIF antibody (1:200, CST, #5318) overnight at 4°C and then incubated with YF 594 goat anti-rabbit IgG (1:200, UElandy) for 2 h, followed by incubation with DAPI (UElandy) for 15 min. .. Finally, cell images were acquired using a confocal laser scanning microscope (Leica).

    Article Title: Disruption of intracellular calcium homeostasis drives graphene quantum dots-induced inflammatory response in liver macrophages
    Article Snippet: Graphene quantum dots (GQDs), with photoluminescent properties, high stability, and excellent biocompatibility, hold tremendous potential in biomedicine.. It is urgent to evaluate their safety and potential health risks to promote their clinical application.. Kupffer cells (KCs), as primary immune cells encountered by foreign substances entering the liver and integral to liver immunity, have yet to be systematically studied for toxicity responses to different GQDs.

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis
    Article Snippet: Various treatment conditions were applied for a duration of 48 h. Post-treatment, the cells were gently rinsed twice with PBS, followed by fixation using Beyotime (China) for 10 min. .. Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China). .. Following washing with PBS, the Cytochrome c oxidase IV (COX IV, CST, USA) -specific primary antibody was applied and incubated overnight.

    Article Title: GPCR-MAPK signaling pathways underpin fitness trade-offs in whitefly
    Article Snippet: .. After washing with PBST for three times, the213 specimens were blocked in Immunol Staining Blocking Buffer Kit (Beyotime, China) at214 4 °C for 4 h, followed by incubation with the primary antibodies against Ex (1:400), Va215 (1:600), Bg (1:400) and Vg (1:400) proteins lasted at 4 °C overnight. .. The samples were216 then incubated against the secondary antibody Alexa Fluor® 555 goat anti-rabbit lgG217 (1:500) (Abcam) for 2 h at room temperature in a black box, and then washed with PBST218 five times.

    Article Title: Veillonella parvula outer membrane vesicles increase ICAM-1 + neutrophils exhibiting elevated NET formation via ROS–PAD4 signaling
    Article Snippet: .. Neutrophils with or without V. parvula OMVw stimulation for 4 h were fixed, permeabilized for 15 min, and blocked using an immunostaining blocking solution (Beyotime). .. Subsequently, the neutrophils were stained with the anti-rabbit ICAM-1 antibody and the anti-goat MPO antibody (R&D Systems, Minneapolis, MN, USA) at 4°C overnight.

    Article Title: The Rheb-mTORC1 Coordinates Cell Cycle Progression and Endoreplication in Bombyx mori
    Article Snippet: And the cells were incubated in a cell culture incubator at 27 ◦C for 2–4 h. The cells were then fixed in 4% paraformaldehyde at room temperature for 15–30 min and incubated with Immunol Staining Wash Buffer (Beyotime, Shanghai, China) for 15 min at room temperature. .. After washing with Immunol Staining Blocking Buffer (Beyotime, Shanghai, China) 3 times, the Click reaction solution was added to each well, and incubated for 30 min in the dark. .. Then the cells were stained with 200 μL of DAPI (Beyotime, Shanghai, China) for 10 min, and were observed by fluorescence microscope (Olympus, Tokyo, Japan).

    Fluorescence:

    Article Title: Zinc ions trigger PANoptosis-like cell death in magnetic hyperthermia therapy of magnesium based implant for hepatocellular carcinoma.
    Article Snippet: According to Global Cancer Statistics 2022, primary liver cancer is the sixth of the top 10 most diagnosed cancers and the third leading cause of cancer death, and more than 75% of the primary liver cancer cases are hepatocellular carcinoma (HCC) [1].. Worsely, the incidence of HCC is increasing year by year worldwide.. In China, there are more than 50% of the world’s newly diagnosed liver cancer cases and deaths, and even over 80% of liver cancer cases are HCC [2].

    Microscopy:

    Article Title: Zinc ions trigger PANoptosis-like cell death in magnetic hyperthermia therapy of magnesium based implant for hepatocellular carcinoma.
    Article Snippet: According to Global Cancer Statistics 2022, primary liver cancer is the sixth of the top 10 most diagnosed cancers and the third leading cause of cancer death, and more than 75% of the primary liver cancer cases are hepatocellular carcinoma (HCC) [1].. Worsely, the incidence of HCC is increasing year by year worldwide.. In China, there are more than 50% of the world’s newly diagnosed liver cancer cases and deaths, and even over 80% of liver cancer cases are HCC [2].

    Immunostaining:

    Article Title: Melittin inhibits MPP + -induced HT22 cell death by suppressing Bax activation and improving mitochondrial function
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min and permeabilised with 0.1% Triton X-100 for 30 min. After being washed with PBS and blocked with immunostaining blocking solution (Beyotime Biotechnology), cells were incubated with anti-AIF antibody (1:200, CST, #5318) overnight at 4°C and then incubated with YF 594 goat anti-rabbit IgG (1:200, UElandy) for 2 h, followed by incubation with DAPI (UElandy) for 15 min. .. Finally, cell images were acquired using a confocal laser scanning microscope (Leica).

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis
    Article Snippet: Various treatment conditions were applied for a duration of 48 h. Post-treatment, the cells were gently rinsed twice with PBS, followed by fixation using Beyotime (China) for 10 min. .. Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China). .. Following washing with PBS, the Cytochrome c oxidase IV (COX IV, CST, USA) -specific primary antibody was applied and incubated overnight.

    Article Title: Veillonella parvula outer membrane vesicles increase ICAM-1 + neutrophils exhibiting elevated NET formation via ROS–PAD4 signaling
    Article Snippet: .. Neutrophils with or without V. parvula OMVw stimulation for 4 h were fixed, permeabilized for 15 min, and blocked using an immunostaining blocking solution (Beyotime). .. Subsequently, the neutrophils were stained with the anti-rabbit ICAM-1 antibody and the anti-goat MPO antibody (R&D Systems, Minneapolis, MN, USA) at 4°C overnight.

    other:

    Article Title: The Rheb-mTORC1 Coordinates Cell Cycle Progression and Endoreplication in Bombyx mori
    Article Snippet: And the cells were incubated in a cell culture incubator at 27 ◦C for 2–4 h. The cells were then fixed in 4% paraformaldehyde at room temperature for 15–30 min and incubated with Immunol Staining Wash Buffer (Beyotime, Shanghai, China) for 15 min at room temperature.



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    Image Search Results


    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Staining

    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Journal: Materials Today Bio

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis

    doi: 10.1016/j.mtbio.2025.102009

    Figure Lengend Snippet: The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Article Snippet: Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China).

    Techniques: Knockdown, Expressing