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immunostaining blocking primary antibody dilution solution  (Sangon Biotech)

 
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    Sangon Biotech immunostaining blocking primary antibody dilution solution
    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
    Immunostaining Blocking Primary Antibody Dilution Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunostaining+blocking+solution/blocking+buffer+immunostaining/pmc12723291-26-0-6
    Average 86 stars, based on 1 article reviews
    immunostaining blocking primary antibody dilution solution - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis"

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    Journal: iScience

    doi: 10.1016/j.isci.2025.114190

    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
    Figure Legend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Techniques Used: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.
    Figure Legend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Techniques Used: Immunostaining, Staining

    Related Articles

    Immunofluorescence:

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4°C overnight.

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts.
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4◦C overnight.

    Blocking Assay:

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4°C overnight.

    Article Title: Blautia coccoides alleviates acute pancreatitis via bile salt hydrolase mediated deoxycholic acid production and farnesoid X receptor signaling.
    Article Snippet: After washing with 520 phosphate-buffered saline (PBS), the sections were outlined with a super pap pen (Biosharp, 521 China) and then treated with immunostaining blocking buffer (Sangon Biotech, China) for 1 522 hour at room temperature. .. Following the blocking step, tissues were incubated overnight at 4°C 523 with primary antibodies targeting claudin1 (ab211737; Abcam, USA), ZO-1 (13663; Cell 524 Signaling Technology, USA), or FGFR4 (11098-1-AP; Proteintech, China) prepared in primary 525 antibody dilution buffer (Sangon Biotech, China). .. The next day, slides were washed with PBS, 526 incubated for 1 hour at room temperature with Alexa Fluor 488/594 AffiniPure donkey anti-527 rabbit IgG (Yeason, China), and washed again.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: .. The samples were subsequently rehydrated in PBS, blocked with goat blocking buffer containing 5% goat serum (Cat. No. E674004, Cat. No. E510009, Sangon Biotech, China), and then incubated with primary antibodies at 4°C overnight, followed by incubation with secondary antibodies at 37°C for 1–2 h. The following primary antibodies were diluted into the above goat-blocking buffer for use: anti-HA high affinity (clone 3F10, rat monoclonal IgG, 1:50; Cat. No. 11867423001; RRID: AB_390918 , Roche, Switzerland), anti-acetylated α-tubulin (clone 6-11B-1, mouse monoclonal IgG, 1:200; Cat. No. T7451; RRID: AB_609894 , Sigma–Aldrich, USA), anti-NPHP4 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China), and anti-NPHP8 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China). .. The secondary antibodies were diluted in secondary antibody dilution buffer containing 5% goat serum (Cat. No. E674005, Cat. No. E510009, Sangon Biotech, China) for use: goat anti-mouse IgG H&L (Alexa Fluor 594), goat anti-rat IgG H&L (Alexa Fluor 488), and goat anti-rabbit IgG H&L (Alexa Fluor 488) (1:500; Cat. No. ab150120; RRID: AB_2631447 , Cat#ab150157; RRID: AB_2722511 , Cat. No. ab150077; RRID: AB_2630356 , Abcam, UK).

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: After repeated washing in PBS, a super pap pen (Sangon Biotech, China) was used to draw a circle around the tissue. .. Slides were blocked with immunostaining blocking buffer (Sangon Biotech, China) at room temperature for 1 h and incubated with primary antibodies against Claudin-1 and ZO-1 dilutedwith primary antibody dilution buffer (Sangon Biotech, China) at 4 °C overnight. .. Slides were washed with PBS and incubated with Alexa Fluor 488 AffiniPure donkey antirabbit IgG (Yeason, China) for 1 h at room temperature.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: DAPI-Fluoromount-G , SouthernBiotech , Cat#0100-20. .. Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004. .. Immunostaining Secondary Antibody Dilution Buffer , Sangon Biotech , Cat#E674005.

    Article Title: Cellular transcriptomics reveals evolutionary adaptation and rumination of vertebrate stomachs
    Article Snippet: .. The sections in 1× citrate-based antigen retrieval solution (Sangon Biotech) were heated in a microwave oven, and stood still for 5 min, before being at 100°C for 5 min. After antigen retrieval, tissue sections were blocked with the Immunostaining Blocking Dilution Buffer (Sangon Biotech) for 1 h at room temperature. .. Subsequently, samples were incubated with primary antibody (1:500, Abcam, ab93279) overnight at 4°C.

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts.
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4◦C overnight.

    Incubation:

    Article Title: Blautia coccoides alleviates acute pancreatitis via bile salt hydrolase mediated deoxycholic acid production and farnesoid X receptor signaling.
    Article Snippet: After washing with 520 phosphate-buffered saline (PBS), the sections were outlined with a super pap pen (Biosharp, 521 China) and then treated with immunostaining blocking buffer (Sangon Biotech, China) for 1 522 hour at room temperature. .. Following the blocking step, tissues were incubated overnight at 4°C 523 with primary antibodies targeting claudin1 (ab211737; Abcam, USA), ZO-1 (13663; Cell 524 Signaling Technology, USA), or FGFR4 (11098-1-AP; Proteintech, China) prepared in primary 525 antibody dilution buffer (Sangon Biotech, China). .. The next day, slides were washed with PBS, 526 incubated for 1 hour at room temperature with Alexa Fluor 488/594 AffiniPure donkey anti-527 rabbit IgG (Yeason, China), and washed again.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: .. The samples were subsequently rehydrated in PBS, blocked with goat blocking buffer containing 5% goat serum (Cat. No. E674004, Cat. No. E510009, Sangon Biotech, China), and then incubated with primary antibodies at 4°C overnight, followed by incubation with secondary antibodies at 37°C for 1–2 h. The following primary antibodies were diluted into the above goat-blocking buffer for use: anti-HA high affinity (clone 3F10, rat monoclonal IgG, 1:50; Cat. No. 11867423001; RRID: AB_390918 , Roche, Switzerland), anti-acetylated α-tubulin (clone 6-11B-1, mouse monoclonal IgG, 1:200; Cat. No. T7451; RRID: AB_609894 , Sigma–Aldrich, USA), anti-NPHP4 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China), and anti-NPHP8 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China). .. The secondary antibodies were diluted in secondary antibody dilution buffer containing 5% goat serum (Cat. No. E674005, Cat. No. E510009, Sangon Biotech, China) for use: goat anti-mouse IgG H&L (Alexa Fluor 594), goat anti-rat IgG H&L (Alexa Fluor 488), and goat anti-rabbit IgG H&L (Alexa Fluor 488) (1:500; Cat. No. ab150120; RRID: AB_2631447 , Cat#ab150157; RRID: AB_2722511 , Cat. No. ab150077; RRID: AB_2630356 , Abcam, UK).

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: After repeated washing in PBS, a super pap pen (Sangon Biotech, China) was used to draw a circle around the tissue. .. Slides were blocked with immunostaining blocking buffer (Sangon Biotech, China) at room temperature for 1 h and incubated with primary antibodies against Claudin-1 and ZO-1 dilutedwith primary antibody dilution buffer (Sangon Biotech, China) at 4 °C overnight. .. Slides were washed with PBS and incubated with Alexa Fluor 488 AffiniPure donkey antirabbit IgG (Yeason, China) for 1 h at room temperature.

    Immunostaining:

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004. .. Immunostaining Secondary Antibody Dilution Buffer , Sangon Biotech , Cat#E674005. .. Goat Serum , Sangon Biotech , Cat#E510009.

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: After repeated washing in PBS, a super pap pen (Sangon Biotech, China) was used to draw a circle around the tissue. .. Slides were blocked with immunostaining blocking buffer (Sangon Biotech, China) at room temperature for 1 h and incubated with primary antibodies against Claudin-1 and ZO-1 dilutedwith primary antibody dilution buffer (Sangon Biotech, China) at 4 °C overnight. .. Slides were washed with PBS and incubated with Alexa Fluor 488 AffiniPure donkey antirabbit IgG (Yeason, China) for 1 h at room temperature.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: DAPI-Fluoromount-G , SouthernBiotech , Cat#0100-20. .. Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004. .. Immunostaining Secondary Antibody Dilution Buffer , Sangon Biotech , Cat#E674005.

    Article Title: Cellular transcriptomics reveals evolutionary adaptation and rumination of vertebrate stomachs
    Article Snippet: .. The sections in 1× citrate-based antigen retrieval solution (Sangon Biotech) were heated in a microwave oven, and stood still for 5 min, before being at 100°C for 5 min. After antigen retrieval, tissue sections were blocked with the Immunostaining Blocking Dilution Buffer (Sangon Biotech) for 1 h at room temperature. .. Subsequently, samples were incubated with primary antibody (1:500, Abcam, ab93279) overnight at 4°C.



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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    Image Search Results


    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Staining

    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Journal: Materials Today Bio

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis

    doi: 10.1016/j.mtbio.2025.102009

    Figure Lengend Snippet: The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Article Snippet: Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China).

    Techniques: Knockdown, Expressing