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hitkit hcs reagent kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hitkit hcs reagent kit
    Hitkit Hcs Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hitkit/us10376514-917-5-9
    Average 86 stars, based on 1 article reviews
    hitkit hcs reagent kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Therapeutic thiophene-, furan-, and pyridine-fused azolopyrimidin-5-(6h)-ones
    Article Snippet: Cells were stained using the HitKitTM HCS reagent kit (Cellomics) according to the manufacturer's instructions (which were previously validated for specific labeling of both neurites and neuronal cell bodies.

    Article Title: Therapeutic thiophene-, furan-, and pyridine-fused azolopyrimidin-5-(6H)-ones
    Article Snippet: Cells were stained using the HitKitTM HCS reagent kit (Cellomics) according to the manufacturer's instructions (which were previously validated for specific labeling of both neurites and neuronal cell bodies.

    Labeling:

    Article Title: Therapeutic thiophene-, furan-, and pyridine-fused azolopyrimidin-5-(6h)-ones
    Article Snippet: Cells were stained using the HitKitTM HCS reagent kit (Cellomics) according to the manufacturer's instructions (which were previously validated for specific labeling of both neurites and neuronal cell bodies.

    Article Title: Therapeutic thiophene-, furan-, and pyridine-fused azolopyrimidin-5-(6H)-ones
    Article Snippet: Cells were stained using the HitKitTM HCS reagent kit (Cellomics) according to the manufacturer's instructions (which were previously validated for specific labeling of both neurites and neuronal cell bodies.



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    Image Search Results


    The effects of TXS inhibition on tumour cell apoptosis . A) Apoptosis was induced in a dose-dependant manner following 24 h treatment with ozagrel, relative to untreated control cells. Cell health following ozagrel treatment was assessed using 3 spectrally distinct flourophores to examine nuclei, f -actin (marker of cytoskeletal integrity), and mitochondrial mass/potential. Reduced f -actin levels demonstrate a loss in cellular integrity during apoptosis. Membrane blebbing also occurs and mitochondrial activity occurs, coupled with a loss of potential across the mitochondrial membrane. These markers were quantified by the Kinetic Scan HCS reader and are represented (B). Similar observations were made in SKMES-1 cells (data not shown). Apoptosis was confirmed following selective TXS inhibition by Cell Death Detection ELISA and DNA laddering in both cell lines (A-549 shown as representative). Cell Death ELISA demonstrated increased apoptosis in a concentration dependant manner, with fold induction expressed as a ratio of control cells (C). DNA laddering was also observed following ozagrel treatment at both 500 nM and 5 μM concentrations (D).

    Journal: Molecular Cancer

    Article Title: Examination of thromboxane synthase as a prognostic factor and therapeutic target in non-small cell lung cancer

    doi: 10.1186/1476-4598-10-25

    Figure Lengend Snippet: The effects of TXS inhibition on tumour cell apoptosis . A) Apoptosis was induced in a dose-dependant manner following 24 h treatment with ozagrel, relative to untreated control cells. Cell health following ozagrel treatment was assessed using 3 spectrally distinct flourophores to examine nuclei, f -actin (marker of cytoskeletal integrity), and mitochondrial mass/potential. Reduced f -actin levels demonstrate a loss in cellular integrity during apoptosis. Membrane blebbing also occurs and mitochondrial activity occurs, coupled with a loss of potential across the mitochondrial membrane. These markers were quantified by the Kinetic Scan HCS reader and are represented (B). Similar observations were made in SKMES-1 cells (data not shown). Apoptosis was confirmed following selective TXS inhibition by Cell Death Detection ELISA and DNA laddering in both cell lines (A-549 shown as representative). Cell Death ELISA demonstrated increased apoptosis in a concentration dependant manner, with fold induction expressed as a ratio of control cells (C). DNA laddering was also observed following ozagrel treatment at both 500 nM and 5 μM concentrations (D).

    Article Snippet: The Multiparamater Apoptosis HitKit from Cellomics provides High Content Screening qualified fluorescent reagents for simultaneous measurement of 3 fundamental parameters of apoptosis.

    Techniques: Inhibition, Marker, Activity Assay, Enzyme-linked Immunosorbent Assay, DNA Laddering, Concentration Assay

    Effect of stable TXS -over-expression on tumour cell survival . Apoptosis was measured in TXS stable transfectants, and corresponding controls (wild-type and empty vector) following 48 h (A) and 72 h (B) serum-starvation by flow cytometry. Representative dot plots following 72 h serum starvation are shown for wild-type (C), empty vector (D) and TXS overexpressing (E) cells. Graphical data is represented at a percentage of the empty vector control, which was set to 100%. Data is expressed as mean ± SEM. n=3 independent experiments.

    Journal: Molecular Cancer

    Article Title: Examination of thromboxane synthase as a prognostic factor and therapeutic target in non-small cell lung cancer

    doi: 10.1186/1476-4598-10-25

    Figure Lengend Snippet: Effect of stable TXS -over-expression on tumour cell survival . Apoptosis was measured in TXS stable transfectants, and corresponding controls (wild-type and empty vector) following 48 h (A) and 72 h (B) serum-starvation by flow cytometry. Representative dot plots following 72 h serum starvation are shown for wild-type (C), empty vector (D) and TXS overexpressing (E) cells. Graphical data is represented at a percentage of the empty vector control, which was set to 100%. Data is expressed as mean ± SEM. n=3 independent experiments.

    Article Snippet: The Multiparamater Apoptosis HitKit from Cellomics provides High Content Screening qualified fluorescent reagents for simultaneous measurement of 3 fundamental parameters of apoptosis.

    Techniques: Over Expression, Plasmid Preparation, Flow Cytometry