Journal:

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

doi: 10.1128/JVI.77.18.10125-10130.2003

Figure Lengend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Article Snippet: BM macrophages were mock or MCMV infected for 18 h and then stimulated with TNF-α (10 ng/ml) for 30 min. Macrophages were then fixed, washed, and stained for NF-κB by using the NF-κB HitKit (Cellomics) per the manufacturer's directions, yielding green color (fluorescein isothiocyanate [FITC]).

Techniques: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

Journal: Molecular Cancer

Article Title: Examination of thromboxane synthase as a prognostic factor and therapeutic target in non-small cell lung cancer

doi: 10.1186/1476-4598-10-25

Figure Lengend Snippet: The effects of TXS inhibition on tumour cell apoptosis . A) Apoptosis was induced in a dose-dependant manner following 24 h treatment with ozagrel, relative to untreated control cells. Cell health following ozagrel treatment was assessed using 3 spectrally distinct flourophores to examine nuclei, f -actin (marker of cytoskeletal integrity), and mitochondrial mass/potential. Reduced f -actin levels demonstrate a loss in cellular integrity during apoptosis. Membrane blebbing also occurs and mitochondrial activity occurs, coupled with a loss of potential across the mitochondrial membrane. These markers were quantified by the Kinetic Scan HCS reader and are represented (B). Similar observations were made in SKMES-1 cells (data not shown). Apoptosis was confirmed following selective TXS inhibition by Cell Death Detection ELISA and DNA laddering in both cell lines (A-549 shown as representative). Cell Death ELISA demonstrated increased apoptosis in a concentration dependant manner, with fold induction expressed as a ratio of control cells (C). DNA laddering was also observed following ozagrel treatment at both 500 nM and 5 μM concentrations (D).

Article Snippet: The Multiparamater Apoptosis HitKit from Cellomics provides High Content Screening qualified fluorescent reagents for simultaneous measurement of 3 fundamental parameters of apoptosis.

Techniques: Inhibition, Marker, Activity Assay, Enzyme-linked Immunosorbent Assay, DNA Laddering, Concentration Assay

Journal: Molecular Cancer

Article Title: Examination of thromboxane synthase as a prognostic factor and therapeutic target in non-small cell lung cancer

doi: 10.1186/1476-4598-10-25

Figure Lengend Snippet: Effect of stable TXS -over-expression on tumour cell survival . Apoptosis was measured in TXS stable transfectants, and corresponding controls (wild-type and empty vector) following 48 h (A) and 72 h (B) serum-starvation by flow cytometry. Representative dot plots following 72 h serum starvation are shown for wild-type (C), empty vector (D) and TXS overexpressing (E) cells. Graphical data is represented at a percentage of the empty vector control, which was set to 100%. Data is expressed as mean ± SEM. n=3 independent experiments.

Article Snippet: The Multiparamater Apoptosis HitKit from Cellomics provides High Content Screening qualified fluorescent reagents for simultaneous measurement of 3 fundamental parameters of apoptosis.

Techniques: Over Expression, Plasmid Preparation, Flow Cytometry

Journal: Molecules

Article Title: Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation

doi: 10.3390/molecules16032583

Figure Lengend Snippet: Fluorescence photomicrographs of panduratin A-induced changes in apoptosis parameters acquired from the ArrayScan HCS Reader . Effects of incubation of A549 cells with panduratin A, compared to controls, respectively, on nuclear area, mitochondrial membrane potential and F-actin. Circular outlines indicate the areas within cells in which fluorescence intensity is measured.

Article Snippet: Apoptosis HitKit TM reagents (Cellomics) was used according to the manufacturer’s instructions.

Techniques: Fluorescence, Incubation

Journal: Molecules

Article Title: Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation

doi: 10.3390/molecules16032583

Figure Lengend Snippet: Quantitative analysis of panduratin A mediated. Dose-response histogram for each apoptosis parameter. Panduratin A increased the percentage of cells with condensed chromatin and F-action content in a concentration-dependent fashion at the same time caused loss of mitochondrial mass/potential.

Article Snippet: Apoptosis HitKit TM reagents (Cellomics) was used according to the manufacturer’s instructions.

Techniques: Concentration Assay