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genechip microarray analysis suite version 4.0 software  (Thermo Fisher)


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    Structured Review

    Thermo Fisher genechip microarray analysis suite version 4.0 software
    Genechip Microarray Analysis Suite Version 4.0 Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+microarray+analysis/pmc03141027-199-5-14
    Average 90 stars, based on 1 article reviews
    genechip microarray analysis suite version 4.0 software - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Fucosylation of HLA-DRB1 regulates CD4 + T cell-mediated anti-melanoma immunity and enhances immunotherapy efficacy
    Article Snippet: All extracted ion chromatograms were generated using Xcalibur Qual Browser version 4.0 (Thermo Fisher Scientific).

    Article Title: Purification, and characterization of a new pro-coagulant protein from Iranian Echis carinatus venom
    Article Snippet: The data were interpreted and processed using Data Explorer version 4.0 (Applied Biosystems) software and Mascot Distiller v.2.8.

    Article Title: Introgression of the QTL qSB11-1 TT conferring sheath blight resistance in rice ( Oryza sativa ) into an elite variety, UKMRC 2, and evaluation of its backcross-derived plants
    Article Snippet: Fragment lengths (allele size) were estimated and scored with GeneMapper version 4.0 analyzing software (Applied Biosystems, USA).

    Article Title: Synthesis and In Vitro Anticancer Evaluation of Flavone—1,2,3-Triazole Hybrids
    Article Snippet: Data acquisition and analysis were accomplished with Xcalibur software version 4.0 (Thermo Fisher Scientific).

    Article Title: Application of DNA forensic to identify a problem leopard and its implications for human-leopard conflict mitigation
    Article Snippet: We manually scored allele sizes in Gene Mapper software version 4.0 (Applied Biosystems, USA).

    Article Title: Development of submicromolar 17β-HSD10 inhibitors and their in vitro and in vivo evaluation.
    Article Snippet: 17β-hydroxysteroid dehydrogenase type 10 (17β-HSD10) is a multifunctional mitochondrial enzyme and putative drug target for the treatment of various pathologies including Alzheimer’s disease or some types of hormonedependent cancer.. In this study, a series of new benzothiazolylurea-based inhibitors were developed based on the structure-activity relationship (SAR) study of previously published compounds and predictions of their physicochemical properties.. This led to the identification of several submicromolar inhibitors (IC50 ~0.3 μM), the most potent compounds within the benzothiazolylurea class known to date.

    Software:

    Article Title: Genetic variation and population structure of Sudanese populations as indicated by 15 Identifiler sequence-tagged repeat (STR) loci
    Article Snippet: For each run on the analyzer, an allelic ladder, positive and negative controls, and an internal lane standard (GeneScan-600 LIZ; Applied Biosystems) were included. .. Allele calling was performed (GeneMapper ID software, version 4.0; Applied Biosystems). ..

    Article Title: Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components
    Article Snippet: .. Acylsugars on leaf surface were collected from similar-sized young leaves by submerging them in 10 mL of extraction solvent, followed by gentle mixing for 2 min. Extracted samples were analyzed using Q Exactive Focus coupled with Ultimate 3000 RS LC unit (Thermo Fisher Scientific) and Exactive Series 2.8 SP1/Xcalibur version 4.0 software. .. Acylsugars were separated by injecting 10 μL of sample into Acclaim 120 (2.1 × 150 mm; 3 μm) C18 column (Thermo Fisher Scientific) that was housed at 30°C.

    Extraction:

    Article Title: Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components
    Article Snippet: .. Acylsugars on leaf surface were collected from similar-sized young leaves by submerging them in 10 mL of extraction solvent, followed by gentle mixing for 2 min. Extracted samples were analyzed using Q Exactive Focus coupled with Ultimate 3000 RS LC unit (Thermo Fisher Scientific) and Exactive Series 2.8 SP1/Xcalibur version 4.0 software. .. Acylsugars were separated by injecting 10 μL of sample into Acclaim 120 (2.1 × 150 mm; 3 μm) C18 column (Thermo Fisher Scientific) that was housed at 30°C.

    Solvent:

    Article Title: Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components
    Article Snippet: .. Acylsugars on leaf surface were collected from similar-sized young leaves by submerging them in 10 mL of extraction solvent, followed by gentle mixing for 2 min. Extracted samples were analyzed using Q Exactive Focus coupled with Ultimate 3000 RS LC unit (Thermo Fisher Scientific) and Exactive Series 2.8 SP1/Xcalibur version 4.0 software. .. Acylsugars were separated by injecting 10 μL of sample into Acclaim 120 (2.1 × 150 mm; 3 μm) C18 column (Thermo Fisher Scientific) that was housed at 30°C.

    Gentle:

    Article Title: Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components
    Article Snippet: .. Acylsugars on leaf surface were collected from similar-sized young leaves by submerging them in 10 mL of extraction solvent, followed by gentle mixing for 2 min. Extracted samples were analyzed using Q Exactive Focus coupled with Ultimate 3000 RS LC unit (Thermo Fisher Scientific) and Exactive Series 2.8 SP1/Xcalibur version 4.0 software. .. Acylsugars were separated by injecting 10 μL of sample into Acclaim 120 (2.1 × 150 mm; 3 μm) C18 column (Thermo Fisher Scientific) that was housed at 30°C.



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    Thermo Fisher genechip microarray analysis suite version 4.0 software
    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the <t>microarray</t> results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.
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    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the <t>microarray</t> results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.
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    Image Search Results


    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface

    doi: 10.1167/iovs.65.6.37

    Figure Lengend Snippet: Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Article Snippet: Microarray experiments were then performed according to the protocols provided by Thermo Fisher Scientific, and scanned microarray images were obtained using the GeneChip Scanner 3000 7G (Thermo Fisher Scientific) microarray analysis system.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing, Microarray