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genechip microarray analysis system gcs 3000dx  (Thermo Fisher)


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    Thermo Fisher genechip microarray analysis system gcs 3000dx
    Genechip Microarray Analysis System Gcs 3000dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+microarray+analysis/pm38336912-119-6-12
    Average 86 stars, based on 1 article reviews
    genechip microarray analysis system gcs 3000dx - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: 18q21.1q21.32 Deletion in a Patient With Juvenile Cerebral Infarction
    Article Snippet: After hybridization, the microarray was washed, stained, and scanned with a GeneChip Scanner 3000Dx v. 2 (Affymetrix, Inc.).

    Article Title: Preliminary study of noninvasive prenatal screening for 22q11.2 deletion/duplication syndrome using multiplex dPCR assay.
    Article Snippet: DNA (250 ng) was amplified, labeled, and hybridized to a GCS 3000Dx v.2 microarray platform (Affymetrix, CA, USA).

    Article Title: Evaluation and Analysis of Absence of Homozygosity (AOH) Using Chromosome Analysis by Medium Coverage Whole Genome Sequencing (CMA-seq) in Prenatal Diagnosis.
    Article Snippet: After washing by the Affymetrix GeneChip® Fluidics Station 450, the arrays were scanned with the GeneChip® System (GCS) 3000Dx.

    Microarray:

    Article Title: A Kpna1-deficient psychotropic drug-induced schizophrenia model mouse for studying gene-environment interactions.
    Article Snippet: Hybridization was performed using the Gene Kit WT PLUS Reagent Kit (Affymetrix, 902280), and microarray analysis was performed using the Clariom S mouse Assay (Applied Biosystems, 902931). .. The data were acquired using the GeneChip microarray analysis system GCS 3000Dx (Affymetrix). .. Microarray data were quality assessed and normalized using Transcriptome Analysis Console (TAC) software (Thermo Fisher); Thermo Fisher array data were normalized using robust multichip analysis (RMA).

    Article Title: Noninvasive Prenatal Screening for 22q11.2 Deletion/Duplication Syndrome Using multiplex dPCR
    Article Snippet: A magnetic beadbased cell-free fetal DNA (cffDNA) enrichment kit (Magnetic Bead-Based cffDNA Puri cation Kit) (Xingzhi Biotechnology, China) was used for enrichment to enhance the proportion of cffDNA. .. CMA DNA (250 ng) was ampli ed, labeled, and hybridized to a GCS 3000Dx v.2 microarray platform (Affymetrix, CA, USA). .. The SNP array test was performed using a commercial 750K microarray chip (Affymetrix CytoScan 750K Array).

    Staining:

    Article Title: Comparison of histopathology to gene expression profiling for the diagnosis of metastatic cancer
    Article Snippet: .. The microarrays were washed and stained using the GeneChip® Hybridization Wash and Stain kit in a GeneChip Fluidics Station FS450Dx, and scanned with a GeneChip Scanner 3000Dx (Affymetrix). ..

    Hybridization:

    Article Title: Comparison of histopathology to gene expression profiling for the diagnosis of metastatic cancer
    Article Snippet: .. The microarrays were washed and stained using the GeneChip® Hybridization Wash and Stain kit in a GeneChip Fluidics Station FS450Dx, and scanned with a GeneChip Scanner 3000Dx (Affymetrix). ..

    Labeling:

    Article Title: Noninvasive Prenatal Screening for 22q11.2 Deletion/Duplication Syndrome Using multiplex dPCR
    Article Snippet: A magnetic beadbased cell-free fetal DNA (cffDNA) enrichment kit (Magnetic Bead-Based cffDNA Puri cation Kit) (Xingzhi Biotechnology, China) was used for enrichment to enhance the proportion of cffDNA. .. CMA DNA (250 ng) was ampli ed, labeled, and hybridized to a GCS 3000Dx v.2 microarray platform (Affymetrix, CA, USA). .. The SNP array test was performed using a commercial 750K microarray chip (Affymetrix CytoScan 750K Array).



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    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface

    doi: 10.1167/iovs.65.6.37

    Figure Lengend Snippet: Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Article Snippet: Microarray experiments were then performed according to the protocols provided by Thermo Fisher Scientific, and scanned microarray images were obtained using the GeneChip Scanner 3000 7G (Thermo Fisher Scientific) microarray analysis system.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing, Microarray