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genechip microarray analysis suite  (Thermo Fisher)


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    Thermo Fisher genechip microarray analysis suite
    Genechip Microarray Analysis Suite, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+microarray+analysis/genechip+microarray+analysis+suite/pm39812341-61-0-1
    Average 90 stars, based on 1 article reviews
    genechip microarray analysis suite - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Host-microbe multi-omics and succinotype profiling have prognostic value for future relapse in patients with inflammatory bowel disease.
    Article Snippet: Standard Affymetrix software (GeneChip Microarray Analysis Suite, ThermoFisher Scientific) was used to capture the scans of the array.

    Article Title: Cervical Microbiome and Response to a Human Papillomavirus Therapeutic Vaccine for Treating High-Grade Cervical Squamous Intraepithelial Lesion
    Article Snippet: Standard Affymetrix software (GeneChip Microarray Analysis Suite, ThermoFisher Scientific) was used to capture the scans.

    Microarray:

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy.
    Article Snippet: The extracted small RNA was quantified using UV absorption at a wavelength of 260 and 280 nm with a spectrophotometer (NanoDrop 3000; Thermo Fisher Scientific, Inc.) and then stored at ‐80 ̊C until further analysis. .. Affymetrix GeneChip microarray (Thermo Fisher Scientific, Inc.) runs were performed on the RNA eluates. .. Intensity values of the CEL files were normalized to remove bias between the arrays using the Robust Multiarray Average and Detection Above BackGround algorithms implemented using the Affymetrix Expression Console software (version 1.4.1; Thermo Fisher Scientific, Inc.).

    Article Title: Transcriptomic Profile of Early Antral Follicles: Predictive Somatic Gene Markers of Oocyte Maturation Outcome.
    Article Snippet: Total RNA was extracted from pools of five follicular walls for each experimental group—namely, GV-stage FWs before hCG stimulation, GV-stage FWs after hCG treatment (unsuccessful), and FWs from MII oocytes that developed to the blastocyst stage (successful) upon IVF—using the Single-Cell RNA Purification Kit (#51800, Norgen Biotek Corp., Thorold, ON, Canada) according to the manufacturer’s protocol. .. The transcriptomic profile analysis was carried out using the MicroArray GeneChip System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). .. More specifically, microarray analysis was conducted on all FW samples using the GeneChip WT PLUS Reagent kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA).



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    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the <t>microarray</t> results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.
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    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface

    doi: 10.1167/iovs.65.6.37

    Figure Lengend Snippet: Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Article Snippet: Microarray experiments were then performed according to the protocols provided by Thermo Fisher Scientific, and scanned microarray images were obtained using the GeneChip Scanner 3000 7G (Thermo Fisher Scientific) microarray analysis system.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing, Microarray