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xbai  (TaKaRa)


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    Structured Review

    TaKaRa xbai
    Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4763 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xbai/Xba+I/pm42103357-61-59-60
    Average 97 stars, based on 4763 article reviews
    xbai - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: Translational regulation by oxidative desulfuration of tRNA modifications.
    Article Snippet: .. Plasmids were linearized by XbaI (Takara) digestion, and mRNAs were synthesized by in vitro transcription with T7 RNA polymerase using a HiScribeTM T7 High Yield RNA Synthesis Kit (NEB). ..

    In Vitro:

    Article Title: Translational regulation by oxidative desulfuration of tRNA modifications.
    Article Snippet: .. Plasmids were linearized by XbaI (Takara) digestion, and mRNAs were synthesized by in vitro transcription with T7 RNA polymerase using a HiScribeTM T7 High Yield RNA Synthesis Kit (NEB). ..

    Purification:

    Article Title: Genetic defects in the CDP-choline pathway for phosphatidylcholine biosynthesis cannot be transmitted to offspring via male gametophytes owing to interruption of autophagy-like processes required for pollen germination in Arabidopsis thaliana
    Article Snippet: PCR products were subjected to 1% agarose gel electrophoresis, and a band for ACA9pro was purified using a gel extraction kit (Wizard SV Gel and PCR Clean-up System, Promega; or Gel/PCR Extraction Kit, FastGene). .. Then, purified ACA 9pro was digested with XbaI (TaKaRa) and HindIII (TaKaRa), and digested samples were subjected to 1% agarose gel electrophoresis and bands were then purified as described above. .. The resultant XbaI–HindIII fragment of ACA9pro was subcloned into the XbaI–HindIII sites of pBluescriptII SK(+) using a 2×Mighty Mix ligation kit (TaKaRa).

    Agarose Gel Electrophoresis:

    Article Title: Genetic defects in the CDP-choline pathway for phosphatidylcholine biosynthesis cannot be transmitted to offspring via male gametophytes owing to interruption of autophagy-like processes required for pollen germination in Arabidopsis thaliana
    Article Snippet: PCR products were subjected to 1% agarose gel electrophoresis, and a band for ACA9pro was purified using a gel extraction kit (Wizard SV Gel and PCR Clean-up System, Promega; or Gel/PCR Extraction Kit, FastGene). .. Then, purified ACA 9pro was digested with XbaI (TaKaRa) and HindIII (TaKaRa), and digested samples were subjected to 1% agarose gel electrophoresis and bands were then purified as described above. .. The resultant XbaI–HindIII fragment of ACA9pro was subcloned into the XbaI–HindIII sites of pBluescriptII SK(+) using a 2×Mighty Mix ligation kit (TaKaRa).

    Plasmid Preparation:

    Article Title: VISTA neutralization by immunization reprograms immunosuppression and augments vaccine efficacy in renal carcinoma.
    Article Snippet: .. 3Wang J, et al. J Immunother Cancer 2026;14:e015052. doi:10.1136/jitc-2026-015052 Plasmid construction and adenoviral vaccine preparation The shuttle vector pCA13 with E1- deleted, Ad5 backbone vector pPE3, pCA13- hCAIX, and pcDNA3.1- mVISTA were maintained in our laboratory.25 Mouse VISTA complementary DNA was excised from the pcDNA3.1- mVISTA plasmid and subcloned into the pCA13 shuttle vector using HindIII (Takara, 1615) and XbaI (Takara, 1634) restriction sites. ..

    Over Expression:

    Article Title: Genome-Wide CRISPR/Cas9 Screening Reveals BmM-ALP Orchestrates the Antioxidant Response and Metabolic Adaptations for Heat Resistance in Bombyx mori .
    Article Snippet: Climate change-induced heat stress represents an increasing threat to biodiversity, engendering the need to understand evolutionary adaptations to thermal extremes.. Using Bombyx mori as an economically important insect and research model, we employed genome-wide CRISPR/Cas9 screening integrated with high-throughput sequencing to systematically identify adaptations for heat resistance.. The analysis identified the Bombyx mori membrane-bound alkaline phosphatase-like gene (BmM-ALP) as a vital thermoregulator.

    Construct:

    Article Title: Genome-Wide CRISPR/Cas9 Screening Reveals BmM-ALP Orchestrates the Antioxidant Response and Metabolic Adaptations for Heat Resistance in Bombyx mori .
    Article Snippet: Climate change-induced heat stress represents an increasing threat to biodiversity, engendering the need to understand evolutionary adaptations to thermal extremes.. Using Bombyx mori as an economically important insect and research model, we employed genome-wide CRISPR/Cas9 screening integrated with high-throughput sequencing to systematically identify adaptations for heat resistance.. The analysis identified the Bombyx mori membrane-bound alkaline phosphatase-like gene (BmM-ALP) as a vital thermoregulator.

    Expressing:

    Article Title: Genome-Wide CRISPR/Cas9 Screening Reveals BmM-ALP Orchestrates the Antioxidant Response and Metabolic Adaptations for Heat Resistance in Bombyx mori .
    Article Snippet: Climate change-induced heat stress represents an increasing threat to biodiversity, engendering the need to understand evolutionary adaptations to thermal extremes.. Using Bombyx mori as an economically important insect and research model, we employed genome-wide CRISPR/Cas9 screening integrated with high-throughput sequencing to systematically identify adaptations for heat resistance.. The analysis identified the Bombyx mori membrane-bound alkaline phosphatase-like gene (BmM-ALP) as a vital thermoregulator.

    CRISPR:

    Article Title: Genome-Wide CRISPR/Cas9 Screening Reveals BmM-ALP Orchestrates the Antioxidant Response and Metabolic Adaptations for Heat Resistance in Bombyx mori .
    Article Snippet: Climate change-induced heat stress represents an increasing threat to biodiversity, engendering the need to understand evolutionary adaptations to thermal extremes.. Using Bombyx mori as an economically important insect and research model, we employed genome-wide CRISPR/Cas9 screening integrated with high-throughput sequencing to systematically identify adaptations for heat resistance.. The analysis identified the Bombyx mori membrane-bound alkaline phosphatase-like gene (BmM-ALP) as a vital thermoregulator.

    Clone Assay:

    Article Title: Genome-Wide CRISPR/Cas9 Screening Reveals BmM-ALP Orchestrates the Antioxidant Response and Metabolic Adaptations for Heat Resistance in Bombyx mori .
    Article Snippet: Climate change-induced heat stress represents an increasing threat to biodiversity, engendering the need to understand evolutionary adaptations to thermal extremes.. Using Bombyx mori as an economically important insect and research model, we employed genome-wide CRISPR/Cas9 screening integrated with high-throughput sequencing to systematically identify adaptations for heat resistance.. The analysis identified the Bombyx mori membrane-bound alkaline phosphatase-like gene (BmM-ALP) as a vital thermoregulator.

    Knock-Out:

    Article Title: Genome-Wide CRISPR/Cas9 Screening Reveals BmM-ALP Orchestrates the Antioxidant Response and Metabolic Adaptations for Heat Resistance in Bombyx mori .
    Article Snippet: Climate change-induced heat stress represents an increasing threat to biodiversity, engendering the need to understand evolutionary adaptations to thermal extremes.. Using Bombyx mori as an economically important insect and research model, we employed genome-wide CRISPR/Cas9 screening integrated with high-throughput sequencing to systematically identify adaptations for heat resistance.. The analysis identified the Bombyx mori membrane-bound alkaline phosphatase-like gene (BmM-ALP) as a vital thermoregulator.



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