xbai Search Results


xbai  (New England Biolabs)
99
New England Biolabs xbai
Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pmc13044897-39-25-26?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
xbai - by Bioz Stars, 2026-08
99/100 stars
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xbai  (Jena Bioscience)
92
Jena Bioscience xbai
Xbai, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pmc08595931__mmc2-243-36-40?v=Jena+Bioscience
Average 92 stars, based on 1 article reviews
xbai - by Bioz Stars, 2026-08
92/100 stars
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xbai restriction sites  (Beyotime)
99
Beyotime xbai restriction sites
Xbai Restriction Sites, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pmc10227425-275-28-36?v=Beyotime
Average 99 stars, based on 1 article reviews
xbai restriction sites - by Bioz Stars, 2026-08
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xbai restriction enzyme new england biolabs cat  (New England Biolabs)
99
New England Biolabs xbai restriction enzyme new england biolabs cat
Xbai Restriction Enzyme New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pm35931073-244-33-36?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
xbai restriction enzyme new england biolabs cat - by Bioz Stars, 2026-08
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prham m xbai p15a aada  (Addgene inc)
91
Addgene inc prham m xbai p15a aada
The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express <t>M.XbaI,</t> M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number <t>p15A</t> origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.
Prham M Xbai P15a Aada, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pmc07489255-95-0-28?v=Addgene+inc
Average 91 stars, based on 1 article reviews
prham m xbai p15a aada - by Bioz Stars, 2026-08
91/100 stars
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restriction enzymes bali, bamhi, draiii, ncoi, xbai, xhoi, and dntp  (Promega)
90
Promega restriction enzymes bali, bamhi, draiii, ncoi, xbai, xhoi, and dntp
The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express <t>M.XbaI,</t> M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number <t>p15A</t> origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.
Restriction Enzymes Bali, Bamhi, Draiii, Ncoi, Xbai, Xhoi, And Dntp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/10__1074_slash_jbc__m107987200-71-9-12?v=Promega
Average 90 stars, based on 1 article reviews
restriction enzymes bali, bamhi, draiii, ncoi, xbai, xhoi, and dntp - by Bioz Stars, 2026-08
90/100 stars
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restriction enzymes sphi, psti, clai, hindiii, saci, xbai and bamhi  (Promega)
90
Promega restriction enzymes sphi, psti, clai, hindiii, saci, xbai and bamhi
The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express <t>M.XbaI,</t> M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number <t>p15A</t> origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.
Restriction Enzymes Sphi, Psti, Clai, Hindiii, Saci, Xbai And Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pm11295459-98-22-32?v=Promega
Average 90 stars, based on 1 article reviews
restriction enzymes sphi, psti, clai, hindiii, saci, xbai and bamhi - by Bioz Stars, 2026-08
90/100 stars
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xbai  (SibEnzyme ltd)
90
SibEnzyme ltd xbai
The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express <t>M.XbaI,</t> M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number <t>p15A</t> origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.
Xbai, supplied by SibEnzyme ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pmc07327921-11-7-8?v=SibEnzyme+ltd
Average 90 stars, based on 1 article reviews
xbai - by Bioz Stars, 2026-08
90/100 stars
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nhei-linker-spei-psti-diphtheria translocation domain-xbai-stop codon-hindiii  (Entelechon GmbH)
90
Entelechon GmbH nhei-linker-spei-psti-diphtheria translocation domain-xbai-stop codon-hindiii
The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express <t>M.XbaI,</t> M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number <t>p15A</t> origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.
Nhei Linker Spei Psti Diphtheria Translocation Domain Xbai Stop Codon Hindiii, supplied by Entelechon GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/us08512984-982-14-21?v=Entelechon+GmbH
Average 90 stars, based on 1 article reviews
nhei-linker-spei-psti-diphtheria translocation domain-xbai-stop codon-hindiii - by Bioz Stars, 2026-08
90/100 stars
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gene between the xbai and bamhi sites  (GenScript corporation)
90
GenScript corporation gene between the xbai and bamhi sites
The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express <t>M.XbaI,</t> M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number <t>p15A</t> origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.
Gene Between The Xbai And Bamhi Sites, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pm36928774-208-5-15?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
gene between the xbai and bamhi sites - by Bioz Stars, 2026-08
90/100 stars
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ntc8685-erna41h-hindiii-xbai expression vector  (Nature Technology Corporation)
90
Nature Technology Corporation ntc8685-erna41h-hindiii-xbai expression vector
INVAC-1 plasmid map. Bases 1–3478: <t>NTC8685-eRNA41H-HindIII-XbaI</t> vector (NTC); Bases 3479–3484: HindIII cloning site (NTC/Invectys); Bases 3485–6967: Ubi-Telomerase transgene (Invectys); Bases 6968–6973: XbaI cloning site (Invectys/NTC); Bases 6974–7120: NTC8685-eRNA41H-HindIII-XbaI vector (NTC).
Ntc8685 Erna41h Hindiii Xbai Expression Vector, supplied by Nature Technology Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/pmc04362362-141-7-12?v=Nature+Technology+Corporation
Average 90 stars, based on 1 article reviews
ntc8685-erna41h-hindiii-xbai expression vector - by Bioz Stars, 2026-08
90/100 stars
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xbai  (Promega)
90
Promega xbai
INVAC-1 plasmid map. Bases 1–3478: <t>NTC8685-eRNA41H-HindIII-XbaI</t> vector (NTC); Bases 3479–3484: HindIII cloning site (NTC/Invectys); Bases 3485–6967: Ubi-Telomerase transgene (Invectys); Bases 6968–6973: XbaI cloning site (Invectys/NTC); Bases 6974–7120: NTC8685-eRNA41H-HindIII-XbaI vector (NTC).
Xbai, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai/10__1128_slash_aem__70__7__4030___4034__2004-52-32-33?v=Promega
Average 90 stars, based on 1 article reviews
xbai - by Bioz Stars, 2026-08
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Image Search Results


The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express M.XbaI, M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number p15A origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.

Journal: PeerJ

Article Title: Methylase-assisted subcloning for high throughput BioBrick assembly

doi: 10.7717/peerj.9841

Figure Lengend Snippet: The lacI-Ptac-lacO insert (A) includes a promoter that is somewhat leaky at high copy number. The IMBB2.4-pUC57-mini backbone (A–B), hereafter abbreviated pUC, is BioBrick-compatible and also includes an NsiI site downstream of PstI . The tagRFP reporter (B) protein can cause colonies to turn visibly pink, but only when the gene encoding it is subcloned downstream of a leaky or constitutive promoter. RP4 oriT-pUC-cat (C) is a BioBrick compatible plasmid that confers resistance to chloramphenicol instead of ampicillin. RP4 oriT serves as a small stuffer in these experiments. In this study this latter plasmid is used only as a recipient plasmid (destination vector) for 3A assembly. Five expression vectors for production of recombinant DNA methyltransferases were constructed for this study. The version that expresses M.Ocy1ORF8430P, a putative ortholog of M.SpeI, is shown (D). The others are similar in design but express M.XbaI, M.EcoRI, M.PstI or M.AvaIII instead. Each plasmid utilizes the low copy number p15A origin (pACYC) and confers resistance to spectinomycin and is thus compatible with pUC plasmids that impart resistance to ampicillin, chloramphenicol or kanamycin. The DNA methyltransferase expression vectors do not contain any of the restriction sites employed in BioBrick assembly protocols (EcoRI, XbaI, SpeI or PstI), so they will not produce restriction fragments that ligate to those that are desired.

Article Snippet: Prham-M.XbaI-p15A-aadA (RRID:Addgene_149338), Prham-M.Ocy1ORF8430P-p15A-aadA (149339), Prham-M.EcoRI-p15A-aadA (149341), Prham-M.AvaIII-p15A-aadA (149342) and Prham-tagRFP-pUC (149343), which was used to optimize the optimal concentration of glucose for auto-induction, have been deposited in the Addgene repository.

Techniques: Plasmid Preparation, Expressing, Recombinant, Construct, Low Copy Number

Model plasmids lacI-Ptac-lacO-pUC and tagRFP-pUC were purified from triplicate cultures of E. coli OmniMax 2 co-transformed with Prham-M.XbaI-p15A-aadA or Prham-M.Ocy1ORF8430P-p15A-aadA respectively. Each purified enzyme was reacted in vitro with XbaI or SpeI-HF, and the extent to which each was cut was assessed by agarose gel electrophoresis. Each of the DNA methyltransferases appears to protect cohabiting plasmid from its corresponding restriction endonuclease, and that protection is sequence specific.

Journal: PeerJ

Article Title: Methylase-assisted subcloning for high throughput BioBrick assembly

doi: 10.7717/peerj.9841

Figure Lengend Snippet: Model plasmids lacI-Ptac-lacO-pUC and tagRFP-pUC were purified from triplicate cultures of E. coli OmniMax 2 co-transformed with Prham-M.XbaI-p15A-aadA or Prham-M.Ocy1ORF8430P-p15A-aadA respectively. Each purified enzyme was reacted in vitro with XbaI or SpeI-HF, and the extent to which each was cut was assessed by agarose gel electrophoresis. Each of the DNA methyltransferases appears to protect cohabiting plasmid from its corresponding restriction endonuclease, and that protection is sequence specific.

Article Snippet: Prham-M.XbaI-p15A-aadA (RRID:Addgene_149338), Prham-M.Ocy1ORF8430P-p15A-aadA (149339), Prham-M.EcoRI-p15A-aadA (149341), Prham-M.AvaIII-p15A-aadA (149342) and Prham-tagRFP-pUC (149343), which was used to optimize the optimal concentration of glucose for auto-induction, have been deposited in the Addgene repository.

Techniques: Purification, Transformation Assay, In Vitro, Agarose Gel Electrophoresis, Plasmid Preparation, Sequencing

INVAC-1 plasmid map. Bases 1–3478: NTC8685-eRNA41H-HindIII-XbaI vector (NTC); Bases 3479–3484: HindIII cloning site (NTC/Invectys); Bases 3485–6967: Ubi-Telomerase transgene (Invectys); Bases 6968–6973: XbaI cloning site (Invectys/NTC); Bases 6974–7120: NTC8685-eRNA41H-HindIII-XbaI vector (NTC).

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Optimization of a gene electrotransfer procedure for efficient intradermal immunization with an hTERT -based DNA vaccine in mice

doi: 10.1038/mtm.2014.45

Figure Lengend Snippet: INVAC-1 plasmid map. Bases 1–3478: NTC8685-eRNA41H-HindIII-XbaI vector (NTC); Bases 3479–3484: HindIII cloning site (NTC/Invectys); Bases 3485–6967: Ubi-Telomerase transgene (Invectys); Bases 6968–6973: XbaI cloning site (Invectys/NTC); Bases 6974–7120: NTC8685-eRNA41H-HindIII-XbaI vector (NTC).

Article Snippet: The ubiquitin-telomerase insert is cloned into the NTC8685-ERNA41H-HindIII-XbaI expression vector designed by Nature Technology Corporation (Lincoln, Nebraska).

Techniques: Plasmid Preparation, Clone Assay