anti vegf  (Proteintech)

Journal: Lab on a Chip

Article Title: Label-free assessment of a microfluidic vessel-on-chip model with visible-light optical tomography reveals structural changes in vascular networks

doi: 10.1039/d5lc00927h

Figure Lengend Snippet: Time-resolved imaging of vascular network development in vessel-on-chip by optical coherence tomography. A) Timeline of disease modeling. Vessel-on-chips were loaded and vessels were allowed to grow for 2 days. Thereafter, the vascular network was subjected to control medium, medium with high glucose and added TNF-α and IL-6, and VEGF medium for 3 more days. Vessel-on-chips were measured every day after day 2. B) Minimum intensity projections, showing the change in the vascular network in the control condition over the course of 5 days. C) Minimum intensity projections, displaying changes in vascular network for the high glucose condition on day 4 and 5. D) Minimum intensity projections, exhibiting changes in the vascular network for the VEGF condition on day 4 and 5. For a full overview of the process, see Fig. S2 in SI. Representative images shown, scale bar = 500 μm.

Article Snippet: On day 3, vascular specification was induced by adding 50 ng ml −1 vascular endothelial growth factor (VEGF) (Miltenyi Biotec, Germany) and 10 μM SB431542 (Tocris Bioscience, UK) in BPEL medium to the cells.

Techniques: Imaging, Tomography, Control

Journal: Lab on a Chip

Article Title: Label-free assessment of a microfluidic vessel-on-chip model with visible-light optical tomography reveals structural changes in vascular networks

doi: 10.1039/d5lc00927h

Figure Lengend Snippet: Change in vessel thickness in the vessel-on-chip over the treatment period, for the different treatments. A) Control condition on day 2 to 5, B) high glucose with added TNF-α and IL-6 condition on day 4 to 5, and C) VEGF treatment on day 4 to 5. For a full overview of the process, see Fig. S3 in SI. Representative images shown, scale bar = 500 μm.

Article Snippet: On day 3, vascular specification was induced by adding 50 ng ml −1 vascular endothelial growth factor (VEGF) (Miltenyi Biotec, Germany) and 10 μM SB431542 (Tocris Bioscience, UK) in BPEL medium to the cells.

Techniques: Control

Journal: Lab on a Chip

Article Title: Label-free assessment of a microfluidic vessel-on-chip model with visible-light optical tomography reveals structural changes in vascular networks

doi: 10.1039/d5lc00927h

Figure Lengend Snippet: Overlay of the variation in the number of branches and vessel length under the different conditions during treatment of the vessel-on-chip for A) the control condition on day 2 to 5, B) the high glucose with added TNF-α and IL-6 condition on day 4 and 5, and C) the VEGF condition on day 4 to 5. For a full overview of the process, see Fig. S4 in SI. Representative images shown, scale bar = 500 μm.

Article Snippet: On day 3, vascular specification was induced by adding 50 ng ml −1 vascular endothelial growth factor (VEGF) (Miltenyi Biotec, Germany) and 10 μM SB431542 (Tocris Bioscience, UK) in BPEL medium to the cells.

Techniques: Control

Journal: Lab on a Chip

Article Title: Label-free assessment of a microfluidic vessel-on-chip model with visible-light optical tomography reveals structural changes in vascular networks

doi: 10.1039/d5lc00927h

Figure Lengend Snippet: Quantitative properties of the vascular network during treatment for all conditions. A) Vascularity index (VI), B) mean thickness, C) total vessel length, and D) number of branching points. Data are presented in boxplots from four individual microfluidic chips ( n = 4). Statistical analyses were performed using one-way ANOVA followed by a Student's t -test. * indicates p < 0.05. E) Minimum intensity projections from Fig. S2 showing the change in the vascular network in the control, high glucose with added TNF-α and IL-6, and VEGF condition over the course of 5 days. Representative images shown, scale bar = 500 μm.

Article Snippet: On day 3, vascular specification was induced by adding 50 ng ml −1 vascular endothelial growth factor (VEGF) (Miltenyi Biotec, Germany) and 10 μM SB431542 (Tocris Bioscience, UK) in BPEL medium to the cells.

Techniques: Control