Journal: Journal of Pain Research

Article Title: Effects and Mechanisms of Wrist-Ankle Acupuncture on Inflammatory Pain in the Rat Gastrocnemius Muscle

doi: 10.2147/JPR.S605568

Figure Lengend Snippet: Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Article Snippet: Complete freund’s adjuvant (CFA) (Sigma, USA, F5881), trichloroethanol (Aibei, Nanjing, CN, M2820), acupuncture needles (Jiajian Medical, CN), TENS-WAA equipment (Shanghai MicroPort Scientific Co, CN), Von Frey hairs mechanical stimulation needles (Yuyan Instrument, CN), Von Frey test cage (Yuyan Instrument, CN), Rat IL-6 ELISA Kit (Servicebio, CN, GER0001-96T), Rat IL-1β ELISA Kit (Servicebio, CN, GER0002-96T), Rat TNF-α ELISA Kit (Servicebio, CN, GER0004-96T), Rat TGF-β1 ELISA Kit (Servicebio, CN, GER0051-96T), BCA Protein Assay Kit (Servicebio, CN, G2026-1000T), TRIzol ® reagent (China, Servicebio, CN, G3013) SweScript All-in-One SuperMix for qPCR (Servicebio, CN, G3337), Blue SYBR Green qPCR Master Mix (Servicebio, CN, G3326).

Techniques:

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: Increased co-expression of OSM or OSMRβ and VEGF correlates with decreased survival of IDC patients. Kaplan–Meier survival curves of invasive ductal breast carcinoma for A) OSM and VEGF (n = 318) and B) OSMRβ and VEGF expression (n = 389), as separated by quartiles. As co-expression of OSM and VEGF increases, overall survival trends decrease, with patients expressing high OSM high VEGF (upper quartiles, respectively) exhibiting significantly worse survival ( P = .0192) than individuals expressing low OSM low VEGF (lower quartiles, respectively). A comparable trend is observed with co-expression of OSMRβ and VEGF ( P = .0012). Analysis obtained from Oncomine dataset entitled Curtis Breast. Log-rank test * P < .05, ** P < .01.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Expressing

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: Inflammatory cytokine and VEGF co-expression are correlated with decreased survival of invasive ductal carcinoma patient. These Kaplan–Meier curves in Figure 1, A and B , are a subset of the data presented in . A) Kaplan–Meier survival curves of invasive ductal breast carcinoma patients with high OSM and VEGF expression (upper quartiles, respectively) present diminished survival when compared to individuals with low OSM and VEGF expression (lower quartiles, respectively). Comparable trends appear upon examination of the upper and lower quartiles of patients with B) OSMRβ and VEGF expression, C) IL-6 and VEGF expression, and D) IL-6Rα and VEGF expression, indicating significant differences between upper and lower quartile survival for each group. Survival of patients with invasive ductal breast carcinoma by E) LIF and VEGF expression and F) LIFRβ and VEGF expression are not significantly different. Analysis obtained from Oncomine dataset entitled Curtis Breast. Log-rank test * P < .05, ** P < .01, *** P < .001. Inflammatory cytokine and VEGF co-expression are correlated with decreased survival of invasive ductal carcinoma patient. These Kaplan–Meier curves in Figure 1, A and B , are a subset of the data presented in Supplemental Figure S1. A) Kaplan–Meier survival curves of invasive ductal breast carcinoma patients with high OSM and VEGF expression (upper quartiles, respectively) present diminished survival when compared to individuals with low OSM and VEGF expression (lower quartiles, respectively). Comparable trends appear upon examination of the upper and lower quartiles of patients with B) OSMRβ and VEGF expression, C) IL-6 and VEGF expression, and D) IL-6Rα and VEGF expression, indicating significant differences between upper and lower quartile survival for each group. Survival of patients with invasive ductal breast carcinoma by E) LIF and VEGF expression and F) LIFRβ and VEGF expression are not significantly different. Analysis obtained from Oncomine dataset entitled Curtis Breast. Log-rank test * P < .05, ** P < .01, *** P < .001.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Expressing

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: Co-expression of VEGF with OSM or OSMRβ appears to affect survival in HER2- but not HER2+ subtypes. A) Kaplan–Meier survival curves of invasive ductal breast carcinoma for HER2- patients and HER2+ patients by OSM and VEGF expression. In HER2- patients, high OSM and high VEGF expression (upper quartiles, respectively) is strongly correlated with decreased survival when compared to patients with low OSM and low VEGF expression (lower quartiles, respectively). However, this trend is not evident in HER2+ individuals, as no significant difference is observed between survival of patients with OSM and VEGF expression in upper and lower quartiles, respectively. Similar trends are observed in Kaplan–Meier survival curves for B) HER2- and HER2+ by OSMRβ and VEGF expression. Analysis obtained from Oncomine dataset entitled Curtis Breast. Log-rank test * P < .05, ** P < .01, *** P < .001.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Expressing

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: IL-6 family cytokine OSM induces VEGF secretion independent of HIF1α signaling in MDA-MB-231 cells. A) Treatment with OSM, IL-6, and LIF (25 ng/mL) for 24 hours promotes expression of HIF1α in MDA-MB-231 TNBC cells. Addition of an siRNA targeting HIF1α (siHIF1α) ablates the effect of adding cytokines. B) T47D cells treated with IL-6 family cytokines for 72 hours express greater levels of HIF1α relative to non-treated control. Treatment with siHIF1α reduces this effect, as assessed by immunoblot analysis. Immunoblots are representative of at least 3 experiments. Induction of VEGF secretion is observed following treatment with IL-6 family cytokines (25 ng/mL) for 24 hours in C) MDA-MB-231 cells (n = 5) and for 72 hours in D) T47D cells (n = 3), as determined by ELISA. Treatment with siHIF1α does not affect VEGF secretion in OSM-treated MDA-MB-231 cells; however, VEGF secretion is modestly reduced in T47D cells treated with OSM and siHIF1α. E) A panel of additional human breast cancer cell lines were tested for VEGF expression by ELISA. HER2- MCF7 cells have significant OSM-induced VEGF secretion, while HER2+ BT474, SK-BR-3, and MDA-MB-453 cells do not produce increased VEGF in response to OSM. One-way ANOVA, Tukey's post-test, ** P < .01, *** P < .001.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Expressing, Control, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: OSM strongly induces phosphorylation of STAT3. A) Treatment with OSM (25 ng/mL) for 15 minutes, 30 minutes, and 1 hour strongly induces phosphorylation of STAT3 (Tyr 705) in MDA-MB-231 cells and moderately induces JNK phosphorylation (T183/Y185). Treatment with either IL-6 or LIF (25 ng/mL) does not induce pSTAT3 or pJNK. B) Treatment with OSM induces phosphorylation of STAT3 and JNK in T47D cells. IL-6 induces moderate phosphorylation of STAT3 and JNK at early time points. Phosphorylation of STAT3 and JNK was compared to control β-actin, as assessed by immunoblot analysis. Blots are representative of three experiments. Time course experiment for STAT3 phosphorylation upon treatment with OSM or IL-6 (25 ng/mL) for 0.25–72 hours in C) MDA-MB-231 cells, and in D) T47D cells. Treatment with IL-6 does not induce pSTAT3 expression in MDA-MB-231 cells, while inducing moderate pSTAT3 expression in T47D cells. ELISA was performed in quadruplicate; two-way ANOVA with Bonferroni post-test, * P < .05, ** P < .01, *** P < .001.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Phospho-proteomics, Control, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: OSM induces VEGF via STAT3 signaling. A) Treatment with OSM (25 ng/mL) and siSTAT3 suppresses VEGF secretion by 3-fold in MDA-MB-231 cells. B) Treatment with OSM and siSTAT3 moderately reduces VEGF secretion by T47D cells. VEGF secretion was not reduced by the addition of siRNAs targeting both JNK1 and JNK2 in either C) MDA-MB-231 or D) T47D cells. Experiments were performed in triplicate two-way ANOVA with Bonferroni post-test, * P < .05, *** P < .001.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques:

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: Mechanistically distinct regulation of VEGF secretion in MDA-MB-231 TNBC and T47D (ER+/PR+/HER2-) cells. A) The VEGF promoter can be activated by various transcription factors. B) In MDA-MB-231 cells, OSM mediates VEGF secretion by activating the STAT3 signaling pathway downstream of the OSMR (OSMRβ + gp130). In T47D cells, OSM regulates VEGF secretion via both HIF1α and the STAT3 signaling pathway.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques:

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: Expression of cytokines or their receptors and VEGF appears to affect survival in HER2- but not HER2+ subtypes. Kaplan–Meier survival curves of invasive ductal breast carcinoma for A) HER2- and HER2+ patients by IL-6 and VEGF expression. In HER2- patients, high IL-6 and high VEGF expression (upper quartiles, respectively) is strongly correlated with decreased survival when compared to patients with low IL-6 and low VEGF expression (lower quartiles, respectively). However, this trend is not evident in HER2+ individuals. Similar trends are observed in Kaplan–Meier survival curves between HER2- and HER2+ patients by B) IL-6Rα and VEGF expression and C) LIF and VEGF expression. D) Co-expression of LIFRβ and VEGF does not significantly affect survival in either HER2- or HER2+ patients. Analysis obtained from Oncomine dataset entitled Curtis Breast. Log-rank test * P < .05, ** P < .01, *** P < .001.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Expressing

Journal: Translational Oncology

Article Title: Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion 1 2

doi: 10.1016/j.tranon.2018.10.004

Figure Lengend Snippet: Co-expression of OSM or OSMRβ and VEGF differentially affects survival based on estrogen receptor status. Kaplan–Meier survival curves of invasive ductal breast carcinoma for A) HER2−/ER- and HER2+/ER- patients and B) HER2−/ER+ and HER2+/ER+ patients by OSM and VEGF expression. In patients with either HER2+/− and ER- expression, OSM/VEGF expression levels do not significantly affect survival. However, in HER2−/ER+ patients, high OSM and high VEGF expression (upper quartiles, respectively) is strongly correlated with decreased survival when compared to patients with similar receptor status expressing low OSM and low VEGF expression (lower quartiles, respectively). Similar trends respective of receptor status are observed in Kaplan–Meier survival curves for C) HER2−/ER- and HER2+/ER- patients and D) HER2−/ER+ and HER2+/ER+ patients by OSMRβ and VEGF expression. While no correlation between OSM/VEGF expression and survival is observed in HER2+/ER+ patients, the low number of samples in these populations distorts our ability to make a robust assessment of this patient subset. Analysis obtained from Oncomine dataset entitled Curtis Breast. Log-rank test * P < .05, ** P < .01.

Article Snippet: Analysis of VEGF secretion in the conditioned media (CM) of MDA-MB-231, T47D, MCF7, SK-BR-3, MDA-MB-453, and BT474 (ATCC, Manassas, VA) breast cancer cells was assessed via VEGF ELISA according to the manufacturer's protocol (Cat# Dy293B, R&D Systems, Bethesda, MD).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Combined Inhibition of Epidermal Growth Factor Receptor and Cyclooxygenase-2 Leads to Greater Anti-tumor Activity of Docetaxel in Advanced Prostate Cancer

doi: 10.1371/journal.pone.0076169

Figure Lengend Snippet: Cells grown in 1% FBS were exposed to 0.01 μmol/L docetaxel (D), 20 μmol/L gefitinib (G) or100 μmol/LNS-398 (N), alone or in combination, or with DMSO as control for 24 h. NF-KB, MMP-9 and VEGF mRNA (A and B) and protein levels (C and D) were measured as described in Materials and Methods. Values are reported as the mean±SD of three independent experiments.

Article Snippet: Antibodies against human VEGF and MMP-9 were obtained from R&D System and Bioworld, respectively.

Techniques: Control

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation