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skeletal muscle differentiation medium  (ATCC)


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    ATCC skeletal muscle differentiation medium
    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic <t>differentiation</t> <t>medium.</t> (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 <t>(muscle</t> system process), and GO:0007519 <t>(skeletal</t> muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
    Skeletal Muscle Differentiation Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool/Skeletal+Muscle+Differentiation+Tool/pmc13091133-107-7-11
    Average 94 stars, based on 21 article reviews
    skeletal muscle differentiation medium - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells"

    Article Title: Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.03.020

    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic differentiation medium. (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 (muscle system process), and GO:0007519 (skeletal muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
    Figure Legend Snippet: RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic differentiation medium. (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 (muscle system process), and GO:0007519 (skeletal muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.

    Techniques Used: RNA Sequencing, Derivative Assay, Cell Characterization, Expressing, Comparison

    Related Articles

    Recombinant:

    Article Title: Protocol for antibody-dependent cellular phagocytosis assay with controlled IgG concentrations in tested samples.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human IgG Fab-specific, 1 mg/mL SouthernBiotech 2085-01 Biological samples Serum from vaccinated seropositive individuals FHCC IRB#10453 Serum from Seattle-area seroepidemiology study participants University of Washington NCT04338360 Pooled anti-SARS-CoV-2 serum University of Washington NCT04344977 Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco 11875-093 Sodium pyruvate Gibco 11360-070 Sodium bicarbonate Gibco 25080-094 HEPES Gibco 15630-080 PenStrep Gibco 15070-063 Fetal bovine serum (FBS) HyClone SH30071.03 Phosphate-buffered solution (PBS) Gibco 14190-144 Tween-20 Sigma-Aldrich P7949-500ML Sodium Azide Millipore Sigma S8032 Paraformaldehyde Boster AR1068 Sulfo-NHS-LC-biotin Thermo Scientific A39257 Sulfo-NHS Thermo Scientific A39269 EDC Thermo Scientific 77149 Trypan Blue stain Invitrogen T10282 Experimental models: Cell lines THP-1 monocytes ATCC TIB-202 Software and algorithms GraphPad Prism GraphPad Software https://www.graphpad.com/how-to-buy/ FlowJo BD Biosciences https://www.flowjo.com/flowjo/download Bio-Plex Manager Bio-Rad https://www.bio-rad.com/en-us/product/ bio-plex-manager-software-standard- edition?ID=5846e84e-03a7-4599a8ae-7ba5dd2c7684 Other Zeba spin desalting column Thermo Scientific 89882 Bio-Plex ProTM Flat Bottom Plates Bio-Rad 171025001 Blotting-grade blocker Bio-Rad 1706404 Tissue Culture Plates, 96-well, U-bottom Genesee Scientific 25-221 NeutrAvidin-labeled microspheres Invitrogen F8776 Bovine serum albumin SAFC Biosciences B4287-100G Bio-Plex Pro Magnetic COOH beads Bio-Rad MC10027-01 Bio-Plex 200 System Bio-Rad N/A BCP Wash buffer Reagent Final concentration Amount Tween-20 (25%) 0.05% 0.4 mL NaN 3 (10%) 0.1% 2 mL Bovine serum albumin 10 mg/mL 2 g PBS N/A 195.6 mL Total N/A 200 mL [Store at room temperature, maximum storage time of 1 month or prepare fresh per experiment]. ..

    Article Title: Chemical modulation of Miro1 alleviates cell-type-specific vulnerabilities in Friedreich's ataxia.
    Article Snippet: Brief communication Chemical modulation of Miro1 alleviates cell-typespecific vulnerabilities in Friedreich’s ataxia

    Staining:

    Article Title: Protocol for antibody-dependent cellular phagocytosis assay with controlled IgG concentrations in tested samples.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human IgG Fab-specific, 1 mg/mL SouthernBiotech 2085-01 Biological samples Serum from vaccinated seropositive individuals FHCC IRB#10453 Serum from Seattle-area seroepidemiology study participants University of Washington NCT04338360 Pooled anti-SARS-CoV-2 serum University of Washington NCT04344977 Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco 11875-093 Sodium pyruvate Gibco 11360-070 Sodium bicarbonate Gibco 25080-094 HEPES Gibco 15630-080 PenStrep Gibco 15070-063 Fetal bovine serum (FBS) HyClone SH30071.03 Phosphate-buffered solution (PBS) Gibco 14190-144 Tween-20 Sigma-Aldrich P7949-500ML Sodium Azide Millipore Sigma S8032 Paraformaldehyde Boster AR1068 Sulfo-NHS-LC-biotin Thermo Scientific A39257 Sulfo-NHS Thermo Scientific A39269 EDC Thermo Scientific 77149 Trypan Blue stain Invitrogen T10282 Experimental models: Cell lines THP-1 monocytes ATCC TIB-202 Software and algorithms GraphPad Prism GraphPad Software https://www.graphpad.com/how-to-buy/ FlowJo BD Biosciences https://www.flowjo.com/flowjo/download Bio-Plex Manager Bio-Rad https://www.bio-rad.com/en-us/product/ bio-plex-manager-software-standard- edition?ID=5846e84e-03a7-4599a8ae-7ba5dd2c7684 Other Zeba spin desalting column Thermo Scientific 89882 Bio-Plex ProTM Flat Bottom Plates Bio-Rad 171025001 Blotting-grade blocker Bio-Rad 1706404 Tissue Culture Plates, 96-well, U-bottom Genesee Scientific 25-221 NeutrAvidin-labeled microspheres Invitrogen F8776 Bovine serum albumin SAFC Biosciences B4287-100G Bio-Plex Pro Magnetic COOH beads Bio-Rad MC10027-01 Bio-Plex 200 System Bio-Rad N/A BCP Wash buffer Reagent Final concentration Amount Tween-20 (25%) 0.05% 0.4 mL NaN 3 (10%) 0.1% 2 mL Bovine serum albumin 10 mg/mL 2 g PBS N/A 195.6 mL Total N/A 200 mL [Store at room temperature, maximum storage time of 1 month or prepare fresh per experiment]. ..

    Software:

    Article Title: Protocol for antibody-dependent cellular phagocytosis assay with controlled IgG concentrations in tested samples.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human IgG Fab-specific, 1 mg/mL SouthernBiotech 2085-01 Biological samples Serum from vaccinated seropositive individuals FHCC IRB#10453 Serum from Seattle-area seroepidemiology study participants University of Washington NCT04338360 Pooled anti-SARS-CoV-2 serum University of Washington NCT04344977 Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco 11875-093 Sodium pyruvate Gibco 11360-070 Sodium bicarbonate Gibco 25080-094 HEPES Gibco 15630-080 PenStrep Gibco 15070-063 Fetal bovine serum (FBS) HyClone SH30071.03 Phosphate-buffered solution (PBS) Gibco 14190-144 Tween-20 Sigma-Aldrich P7949-500ML Sodium Azide Millipore Sigma S8032 Paraformaldehyde Boster AR1068 Sulfo-NHS-LC-biotin Thermo Scientific A39257 Sulfo-NHS Thermo Scientific A39269 EDC Thermo Scientific 77149 Trypan Blue stain Invitrogen T10282 Experimental models: Cell lines THP-1 monocytes ATCC TIB-202 Software and algorithms GraphPad Prism GraphPad Software https://www.graphpad.com/how-to-buy/ FlowJo BD Biosciences https://www.flowjo.com/flowjo/download Bio-Plex Manager Bio-Rad https://www.bio-rad.com/en-us/product/ bio-plex-manager-software-standard- edition?ID=5846e84e-03a7-4599a8ae-7ba5dd2c7684 Other Zeba spin desalting column Thermo Scientific 89882 Bio-Plex ProTM Flat Bottom Plates Bio-Rad 171025001 Blotting-grade blocker Bio-Rad 1706404 Tissue Culture Plates, 96-well, U-bottom Genesee Scientific 25-221 NeutrAvidin-labeled microspheres Invitrogen F8776 Bovine serum albumin SAFC Biosciences B4287-100G Bio-Plex Pro Magnetic COOH beads Bio-Rad MC10027-01 Bio-Plex 200 System Bio-Rad N/A BCP Wash buffer Reagent Final concentration Amount Tween-20 (25%) 0.05% 0.4 mL NaN 3 (10%) 0.1% 2 mL Bovine serum albumin 10 mg/mL 2 g PBS N/A 195.6 mL Total N/A 200 mL [Store at room temperature, maximum storage time of 1 month or prepare fresh per experiment]. ..

    Article Title: Chemical modulation of Miro1 alleviates cell-type-specific vulnerabilities in Friedreich's ataxia.
    Article Snippet: Brief communication Chemical modulation of Miro1 alleviates cell-typespecific vulnerabilities in Friedreich’s ataxia

    Concentration Assay:

    Article Title: Protocol for antibody-dependent cellular phagocytosis assay with controlled IgG concentrations in tested samples.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human IgG Fab-specific, 1 mg/mL SouthernBiotech 2085-01 Biological samples Serum from vaccinated seropositive individuals FHCC IRB#10453 Serum from Seattle-area seroepidemiology study participants University of Washington NCT04338360 Pooled anti-SARS-CoV-2 serum University of Washington NCT04344977 Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco 11875-093 Sodium pyruvate Gibco 11360-070 Sodium bicarbonate Gibco 25080-094 HEPES Gibco 15630-080 PenStrep Gibco 15070-063 Fetal bovine serum (FBS) HyClone SH30071.03 Phosphate-buffered solution (PBS) Gibco 14190-144 Tween-20 Sigma-Aldrich P7949-500ML Sodium Azide Millipore Sigma S8032 Paraformaldehyde Boster AR1068 Sulfo-NHS-LC-biotin Thermo Scientific A39257 Sulfo-NHS Thermo Scientific A39269 EDC Thermo Scientific 77149 Trypan Blue stain Invitrogen T10282 Experimental models: Cell lines THP-1 monocytes ATCC TIB-202 Software and algorithms GraphPad Prism GraphPad Software https://www.graphpad.com/how-to-buy/ FlowJo BD Biosciences https://www.flowjo.com/flowjo/download Bio-Plex Manager Bio-Rad https://www.bio-rad.com/en-us/product/ bio-plex-manager-software-standard- edition?ID=5846e84e-03a7-4599a8ae-7ba5dd2c7684 Other Zeba spin desalting column Thermo Scientific 89882 Bio-Plex ProTM Flat Bottom Plates Bio-Rad 171025001 Blotting-grade blocker Bio-Rad 1706404 Tissue Culture Plates, 96-well, U-bottom Genesee Scientific 25-221 NeutrAvidin-labeled microspheres Invitrogen F8776 Bovine serum albumin SAFC Biosciences B4287-100G Bio-Plex Pro Magnetic COOH beads Bio-Rad MC10027-01 Bio-Plex 200 System Bio-Rad N/A BCP Wash buffer Reagent Final concentration Amount Tween-20 (25%) 0.05% 0.4 mL NaN 3 (10%) 0.1% 2 mL Bovine serum albumin 10 mg/mL 2 g PBS N/A 195.6 mL Total N/A 200 mL [Store at room temperature, maximum storage time of 1 month or prepare fresh per experiment]. ..

    Cell Characterization:

    Article Title: Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells
    Article Snippet: .. For HSkMCs, myogenic differentiation was induced using Skeletal Muscle Differentiation Medium (ATCC, Manassas, USA). ..

    Control:

    Article Title: Chemical modulation of Miro1 alleviates cell-type-specific vulnerabilities in Friedreich's ataxia.
    Article Snippet: Brief communication Chemical modulation of Miro1 alleviates cell-typespecific vulnerabilities in Friedreich’s ataxia



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    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic <t>differentiation</t> <t>medium.</t> (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 <t>(muscle</t> system process), and GO:0007519 <t>(skeletal</t> muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
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    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic <t>differentiation</t> <t>medium.</t> (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 <t>(muscle</t> system process), and GO:0007519 <t>(skeletal</t> muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
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    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic <t>differentiation</t> <t>medium.</t> (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 <t>(muscle</t> system process), and GO:0007519 <t>(skeletal</t> muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
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    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic <t>differentiation</t> <t>medium.</t> (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 <t>(muscle</t> system process), and GO:0007519 <t>(skeletal</t> muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
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    Image Search Results


    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic differentiation medium. (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 (muscle system process), and GO:0007519 (skeletal muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.

    Journal: Bioactive Materials

    Article Title: Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells

    doi: 10.1016/j.bioactmat.2026.03.020

    Figure Lengend Snippet: RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic differentiation medium. (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 (muscle system process), and GO:0007519 (skeletal muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.

    Article Snippet: For HSkMCs, myogenic differentiation was induced using Skeletal Muscle Differentiation Medium (ATCC, Manassas, USA).

    Techniques: RNA Sequencing, Derivative Assay, Cell Characterization, Expressing, Comparison