ticam2  (Millipore)

Journal: Stem Cells International

Article Title: Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway

doi: 10.1155/2017/5841814

Figure Lengend Snippet: EA treatment exerted an inhibitory effect on both of the two downstream pathways of TLR4 in the hippocampus posttrauma. Hippocampal TLR4, Myd88, TRAF6, TRAM, TRIF, and NF- κ B expression of the sham, TBI, TBI + EA, TBI + EA + LPS, TBI + EA + Veh, and sham + EA groups ( n = 3 in each group) were detected by WB at 35 days after TBI. (a–f) The six kinds of proteins in TLR4/Myd88 κ B and TLR4/TRIF/ κ B signaling pathways were upregualted in the TBI group compared with the sham group and the sham + EA group. EA treatment resulted in a significant decrease in the TBI + EA group, but the effects were attenuated by LPS administration in the TBI + EA + LPS group. No difference of the proteins between the TBI + EA and TBI + EA + Veh groups was observed. ∗ P < 0.05 versus the TBI group, † P < 0.05 versus the TBI + EA + LPS group.

Article Snippet: Membranes were blocked with 5% nonfat milk solution in tris-buffered saline with 0.1% Triton X-100 (TBST) for 1 hour and then incubated overnight at 4°C with appropriate primary antibodies as below: rabbit anti-mouse TLR4 antibody (1 : 1000, Thermo Fisher, PA5-23125, Rockford, IL, USA), rabbit anti-mouse myeloid differentiation factor 88 (Myd88) antibody (1 : 500, Santa Cruz, sc-17320, Dallas, TX, USA), rabbit anti-mouse TNFR-associated factor (TRAF6) antibody (1 : 1000, Novus Biological, NB100-56179, Littleton, CO, USA), rabbit anti-mouse toll/IL-1 receptor domain-containing adapter-induced interferon- β (TRIF) antibody (1 : 1000, Enzo Life Sciences, ALX-215-016, Farmingdale, NY, USA), rabbit anti-mouse TRIF-related adaptor molecule (TRAM) antibody (1 : 1000, OriGene, TA-306163, Rockville, MD, USA), rabbit anti-mouse nuclear factor- κ B (NF- κ B) p65 antibody (1 : 1000, GeneTex, GTX21893, Irvine, CA, USA), and rabbit anti- β -actin antibody (1 : 2000, Proteintech, 20536-1-AP, Rosemont, IL, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

doi: 10.1371/journal.pone.0033625

Figure Lengend Snippet: Primers used for qPCR analysis.

Article Snippet: After performing microarray experiments and bioinformatic data analysis, three transcripts were selected to validate the results: RDX (radixin; Affymetrix probe Ssc.21016.1.S1_at; G = Ssc.21016), SLCO1A2 (solute carrier organic anion transporter family, member 1A2; Affymetrix probe Ssc.16331.1.S1_at, AF403248.1) and TICAM2 (TIR domain- containing adapter molecule 2; Affymetrix probe Ssc.2201.2.S1_at; BX670675).

Techniques:

Journal: PLoS ONE

Article Title: Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

doi: 10.1371/journal.pone.0033625

Figure Lengend Snippet: RDX (radixin), SLCO1A2 (solute carrier organic anion transporter family member 1A2) and TICAM2 (TIR domain- containing adapter molecule 2) expression values (normalized based on ß–actin expression values) in uterine horn samples in the presence of embryos at the blastocyst stage (Inseminated) compared to the presence of oocytes (Non Inseminated). The expression of all transcripts in the uterine horn in the presence of blastocyst was significantly different from that of oocyte in the uterine horn (P<0.05).

Article Snippet: After performing microarray experiments and bioinformatic data analysis, three transcripts were selected to validate the results: RDX (radixin; Affymetrix probe Ssc.21016.1.S1_at; G = Ssc.21016), SLCO1A2 (solute carrier organic anion transporter family, member 1A2; Affymetrix probe Ssc.16331.1.S1_at, AF403248.1) and TICAM2 (TIR domain- containing adapter molecule 2; Affymetrix probe Ssc.2201.2.S1_at; BX670675).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

doi: 10.1371/journal.pone.0033625

Figure Lengend Snippet: Differentially expressed transcripts and pathways involved in Signal transduction, Cell communication and immune system.

Article Snippet: After performing microarray experiments and bioinformatic data analysis, three transcripts were selected to validate the results: RDX (radixin; Affymetrix probe Ssc.21016.1.S1_at; G = Ssc.21016), SLCO1A2 (solute carrier organic anion transporter family, member 1A2; Affymetrix probe Ssc.16331.1.S1_at, AF403248.1) and TICAM2 (TIR domain- containing adapter molecule 2; Affymetrix probe Ssc.2201.2.S1_at; BX670675).

Techniques: Transduction, Coagulation, Migration

Journal: PLoS ONE

Article Title: Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

doi: 10.1371/journal.pone.0033625

Figure Lengend Snippet: TICAM2 expression values (normalized based on ß–actin expression values) in oviduct ( A ) and UH samples ( B ) in the presence of embryos at different stages of development (Inseminated) and in the presence of oocytes (Non inseminated). Embryo was located in oviduct at 2 cells and 4 cells embryo stage; Embryo was located in uterine horn at 4 cells, morula and blastocyst). *(P<0.05).

Article Snippet: After performing microarray experiments and bioinformatic data analysis, three transcripts were selected to validate the results: RDX (radixin; Affymetrix probe Ssc.21016.1.S1_at; G = Ssc.21016), SLCO1A2 (solute carrier organic anion transporter family, member 1A2; Affymetrix probe Ssc.16331.1.S1_at, AF403248.1) and TICAM2 (TIR domain- containing adapter molecule 2; Affymetrix probe Ssc.2201.2.S1_at; BX670675).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Early Developing Pig Embryos Mediate Their Own Environment in the Maternal Tract

doi: 10.1371/journal.pone.0033625

Figure Lengend Snippet: TICAM2 expression values (normalized based on ß–actin expression values) in oviduct ( A ) and UH samples ( B ) in the presence of embryos at different stages of development (Inseminated) and in the presence of oocytes (Non inseminated). *(P<0.05).

Article Snippet: After performing microarray experiments and bioinformatic data analysis, three transcripts were selected to validate the results: RDX (radixin; Affymetrix probe Ssc.21016.1.S1_at; G = Ssc.21016), SLCO1A2 (solute carrier organic anion transporter family, member 1A2; Affymetrix probe Ssc.16331.1.S1_at, AF403248.1) and TICAM2 (TIR domain- containing adapter molecule 2; Affymetrix probe Ssc.2201.2.S1_at; BX670675).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

doi: 10.3389/fphar.2021.753599

Figure Lengend Snippet: Primer sequences for PCR.

Article Snippet: Antibodies against Toll-like receptor 2 (TLR2), cluster of differentiation 14 (CD14), and TRIF-related adaptor molecule (TRAM) were purchased from Proteintech (IL, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

doi: 10.3389/fphar.2021.753599

Figure Lengend Snippet: ABPS increased the expression of TLR4 and MyD88 in J774 A.1 cells. (A) The mRNA expression of TLR2, TLR4, TRAM, and MyD88. (B) The protein expression of TLR2, TLR4, TRAM, and MyD88. The blot shown is representative of one of the three similar experiments. Cells were treated 500 μg/ml ABPS for 24 h. NC: normal control; ABPS500: 500 μg/ml ABPS treated. * p < 0.05, ** p < 0.01 vs. NC. The values are presented as means ± SD.

Article Snippet: Antibodies against Toll-like receptor 2 (TLR2), cluster of differentiation 14 (CD14), and TRIF-related adaptor molecule (TRAM) were purchased from Proteintech (IL, United States).

Techniques: Expressing

Journal: Brain, behavior, and immunity

Article Title: Distinct cellular mediators drive the Janus Faces of Toll-like Receptor 4 regulation of network excitability which impacts working memory performance after brain Injury

doi: 10.1016/j.bbi.2020.03.035

Figure Lengend Snippet: Antibodies

Article Snippet: Densitometric quantification was determined using Image-J software (NIH) and normalized to β-actin density. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Molecular Type Weight Dilution Company Catalog Number Figure # MAP2 Chicken Polyclonal 1:1000 AbCam ab5392 GFAP Mouse monoclonal 1:1000 Millipore MAB360 Data not shown IBA-1 Mouse monoclonal 1:1000 Millipore MABN92 Data not shown β-actin 42kDa Mouse Monoclonal 1:5000 Sigma-Aldrich A1978 HMGB1 29kDa Rabbit Polyclonal 1:1000 Abcam AB18256 MyD88 35kDa Rabbit Polyclonal 1:1000 Abcam Ab2064 IKB 40kDa Mouse Monoclonal 1:500 Novus 6A920 (NB100-56507) NFkB 60kDa Rabbit Polyclonal 1:1000 Abcam Ab16502 TICAM2 32kDa Rabbit Polyclonal 1:500 Millipore ABF235 IRF3 47kDa Rabbit Polyclonal 1:1000 Abcam Ab25950 TNFa 25kDa Rabbit Polyclonal 1:1000 Abcam Ab9635 Open in a separate window Antibodies 2.6.

Techniques:

Journal: Brain, behavior, and immunity

Article Title: Distinct cellular mediators drive the Janus Faces of Toll-like Receptor 4 regulation of network excitability which impacts working memory performance after brain Injury

doi: 10.1016/j.bbi.2020.03.035

Figure Lengend Snippet: Representative western blots of HMGB1 (A) MyD88, IkBα, and NFκB, (C) and, TICAM2, IRF3 and TNFα (G) in hippocampal samples from the injured side obtained 3 days after vehicle/CLI-095 treatment. Treatments were started 24 hours after injury. Corresponding β-actin bands are illustrated. (A, C and G) Summary histograms of expression of HMGB1 (B), MyD88 (D), IkBα (E), NFκB (F), TICAM2 (H), IRF3 (I) and TNFα (J), normalized to the expression levels in sham-vehicle treated controls. * indicates p<0.05 compared to sham and # indicates p<0.05 compared to corresponding ACSF by TW ANOVA followed by post-hoc Tukey’s test.

Article Snippet: Densitometric quantification was determined using Image-J software (NIH) and normalized to β-actin density. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Molecular Type Weight Dilution Company Catalog Number Figure # MAP2 Chicken Polyclonal 1:1000 AbCam ab5392 GFAP Mouse monoclonal 1:1000 Millipore MAB360 Data not shown IBA-1 Mouse monoclonal 1:1000 Millipore MABN92 Data not shown β-actin 42kDa Mouse Monoclonal 1:5000 Sigma-Aldrich A1978 HMGB1 29kDa Rabbit Polyclonal 1:1000 Abcam AB18256 MyD88 35kDa Rabbit Polyclonal 1:1000 Abcam Ab2064 IKB 40kDa Mouse Monoclonal 1:500 Novus 6A920 (NB100-56507) NFkB 60kDa Rabbit Polyclonal 1:1000 Abcam Ab16502 TICAM2 32kDa Rabbit Polyclonal 1:500 Millipore ABF235 IRF3 47kDa Rabbit Polyclonal 1:1000 Abcam Ab25950 TNFa 25kDa Rabbit Polyclonal 1:1000 Abcam Ab9635 Open in a separate window Antibodies 2.6.

Techniques: Western Blot, Expressing