Journal: Stem Cells International

Article Title: Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway

doi: 10.1155/2017/5841814

Figure Lengend Snippet: EA treatment exerted an inhibitory effect on both of the two downstream pathways of TLR4 in the hippocampus posttrauma. Hippocampal TLR4, Myd88, TRAF6, TRAM, TRIF, and NF- κ B expression of the sham, TBI, TBI + EA, TBI + EA + LPS, TBI + EA + Veh, and sham + EA groups ( n = 3 in each group) were detected by WB at 35 days after TBI. (a–f) The six kinds of proteins in TLR4/Myd88 κ B and TLR4/TRIF/ κ B signaling pathways were upregualted in the TBI group compared with the sham group and the sham + EA group. EA treatment resulted in a significant decrease in the TBI + EA group, but the effects were attenuated by LPS administration in the TBI + EA + LPS group. No difference of the proteins between the TBI + EA and TBI + EA + Veh groups was observed. ∗ P < 0.05 versus the TBI group, † P < 0.05 versus the TBI + EA + LPS group.

Article Snippet: Membranes were blocked with 5% nonfat milk solution in tris-buffered saline with 0.1% Triton X-100 (TBST) for 1 hour and then incubated overnight at 4°C with appropriate primary antibodies as below: rabbit anti-mouse TLR4 antibody (1 : 1000, Thermo Fisher, PA5-23125, Rockford, IL, USA), rabbit anti-mouse myeloid differentiation factor 88 (Myd88) antibody (1 : 500, Santa Cruz, sc-17320, Dallas, TX, USA), rabbit anti-mouse TNFR-associated factor (TRAF6) antibody (1 : 1000, Novus Biological, NB100-56179, Littleton, CO, USA), rabbit anti-mouse toll/IL-1 receptor domain-containing adapter-induced interferon- β (TRIF) antibody (1 : 1000, Enzo Life Sciences, ALX-215-016, Farmingdale, NY, USA), rabbit anti-mouse TRIF-related adaptor molecule (TRAM) antibody (1 : 1000, OriGene, TA-306163, Rockville, MD, USA), rabbit anti-mouse nuclear factor- κ B (NF- κ B) p65 antibody (1 : 1000, GeneTex, GTX21893, Irvine, CA, USA), and rabbit anti- β -actin antibody (1 : 2000, Proteintech, 20536-1-AP, Rosemont, IL, USA).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

doi: 10.3389/fphar.2021.753599

Figure Lengend Snippet: Primer sequences for PCR.

Article Snippet: Antibodies against Toll-like receptor 2 (TLR2), cluster of differentiation 14 (CD14), and TRIF-related adaptor molecule (TRAM) were purchased from Proteintech (IL, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

doi: 10.3389/fphar.2021.753599

Figure Lengend Snippet: ABPS increased the expression of TLR4 and MyD88 in J774 A.1 cells. (A) The mRNA expression of TLR2, TLR4, TRAM, and MyD88. (B) The protein expression of TLR2, TLR4, TRAM, and MyD88. The blot shown is representative of one of the three similar experiments. Cells were treated 500 μg/ml ABPS for 24 h. NC: normal control; ABPS500: 500 μg/ml ABPS treated. * p < 0.05, ** p < 0.01 vs. NC. The values are presented as means ± SD.

Article Snippet: Antibodies against Toll-like receptor 2 (TLR2), cluster of differentiation 14 (CD14), and TRIF-related adaptor molecule (TRAM) were purchased from Proteintech (IL, United States).

Techniques: Expressing

Journal: Neural Plasticity

Article Title: Blockade of Toll-Like Receptors (TLR2, TLR4) Attenuates Pain and Potentiates Buprenorphine Analgesia in a Rat Neuropathic Pain Model

doi: 10.1155/2016/5238730

Figure Lengend Snippet: Quantitative real-time PCR analysis of CD40 (a, b), TLR2 (c, d), TLR4 (e, f), MyD88 (g, h), and TICAM2 (i, j) mRNA levels in the ipsilateral dorsal lumbar spinal cord (a, c, e, g, and i) and DRG (b, d, f, h, and j) tissue on 2nd, 7th, and 14th days after chronic constriction injury (CCI) in rats. The data are presented as the mean ± SEM, which represent normalized averages derived from the threshold cycles obtained in qRT-PCR from 6–8 samples per group. Intergroup differences were analyzed using one-way ANOVA followed by Bonferroni's multiple comparisons test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences versus the INTACT group.

Article Snippet: Rn01527838_g1 ( Hprt ), Rn00569848_m1 ( Tlr4 ), Rn02133647_m1 ( Tlr2 ), Rn01640049_m1 ( MyD88 ), Rn02082474_s1 ( Ticam2 ), and Rn01423590_m1 ( CD40 ) were used as TaqMan primers and probes.

Techniques: Real-time Polymerase Chain Reaction, Derivative Assay, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: A Toll-Like Receptor 2 Pathway Regulates the Ppargc1a/b Metabolic Co-Activators in Mice with Staphylococcal aureus Sepsis

doi: 10.1371/journal.pone.0025249

Figure Lengend Snippet: Antibodies and Primers.

Article Snippet: TICAM-2 (TRAM) , Rabbit , Santa Cruz , 67061.

Techniques: