sta21 (Santa Cruz Biotechnology)
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Sta21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sta21/STA-21/pm35820245-47-0-4
Average 93 stars, based on 44 article reviews
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Cell Culture:Article Title: The Therapeutic Effect of STAT3 Signaling-Suppressed MSC on Pain and Articular Cartilage Damage in a Rat Model of Monosodium Iodoacetate-Induced Osteoarthritis Article Snippet: Cells were resuspended and cultured with Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Pan-Biotech, Aidenbach, Germany). .. The cells were cultured for 4 days until 90% confluent (passage 0) and were then expanded for 2–3 passages and used for experiments. iSTAT3 OA-MSCs were prepared by treatment of OA-MSCs with 10 μM Transduction:Article Title: ADAM9 enhances Th17 cell differentiation and autoimmunity by activating TGF-β1 Article Snippet: In addition to Th0-nonpolarizing conditions, the following stimulations were used for each polarizing condition: IL-12 (20 ng/mL, R&D Systems) and anti-IL-4 (10 μg/mL, C17.8; Bio X Cell) for Th1 condition; IL-4 (100 ng/mL, R&D Systems) and anti-IFNγ (10 μg/mL, XMG1.2; Bio X Cell) for Th2 condition; IL-6 (3 ng/mL, R&D Systems), TGF-b1 (0.3 ng/mL, R&D Systems), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Th17; IL-6 (3 ng/mL), latent TGF-b1 (1.0 ng/mL, R&D Systems), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Th17 with latent TGF- β1 condition; and IL-2 (20 ng/mL, R&D Systems), TGF-β1 (1.0 ng/mL, R&D Systems), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Tregs condition; and IL-2 (20 ng/mL), latent TGF-β1 (3.0 ng/mL), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Tregs with latent TGF-β1 condition. .. For signal transduction studies, Binding Assay:Article Title: STAT3 Phosphorylation Mediating DMSO's Function on Fetal Cardiomyocyte Proliferation with Developmental Changes. Article Snippet: Ventricles were enzymatically dissociated to get single cells as previously described.24-26) The isolated cells were plated on sterile gelatin-coated glass cover slips, cultured in Dulbecco’s Modified Eagle’s Medium (Gibco) containing 20% fetal bovine serum (Gibco), and kept in the incubator for 24 hours for further treatment according to the following experiments. .. DMSO (from 1:100,000 to 1:1000, Sigma, USA), Inhibition:Article Title: Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates Article Snippet: .. Inhibition of STAT3 signaling in MSCs (iSTAT3-MSCs) iSTAT3-MSCs were obtained by adding 10 μM Enzyme-linked Immunosorbent Assay:Article Title: Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates Article Snippet: .. Inhibition of STAT3 signaling in MSCs (iSTAT3-MSCs) iSTAT3-MSCs were obtained by adding 10 μM |
