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sta21  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology sta21
    Sta21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sta21/STA-21/pm35820245-47-0-4
    Average 93 stars, based on 44 article reviews
    sta21 - by Bioz Stars, 2026-10
    93/100 stars

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    other:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: Lymphocytes were isolated from processed tumor tissues by OptiPrep (Sigma) density gradient centrifugation.

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: For isolation of tumor-infiltrating lymphocytes, tumor tissuewasminced into 1 to 2 mm pieces and digested with collagenase IV (1.25 mg/mL; #LS004188, Worthington), 0.1% soybean trypsin inhibitor (#T9128, Sigma), hyaluronidase (1 mg/mL; #LS002592, Worthington), and DNase I (100 mg/mL; #LS002007, Worthington) in complete DMEM for 30minutes at 37 C. Cell suspensionswere passed through a 70-mmcell strainer (Falcon) and resuspended in RPMImedia (Gibco).

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity
    Article Snippet: STA-21 (STAT3 inhibitor) , Santa Cruz Biotechnology , Cat# SC-200757.

    MTT Assay:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: IL-6 R & D System Cat# 406-ML/CF Rec. .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022 .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022

    In Situ:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: IL-6 R & D System Cat# 406-ML/CF Rec. .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022 .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022

    Proximity Ligation Assay:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: IL-6 R & D System Cat# 406-ML/CF Rec. .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022 .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022

    Enzyme-linked Immunosorbent Assay:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: IL-6 R & D System Cat# 406-ML/CF Rec. .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022 .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022

    Extraction:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: IL-6 R & D System Cat# 406-ML/CF Rec. .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022 .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022

    Cytotoxicity Assay:

    Article Title: Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.
    Article Snippet: IL-6 R & D System Cat# 406-ML/CF Rec. .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022 .. IL-1b R & D System Cat# 401-ML/CF STA-21 (STAT3 inhibitor) Santa Cruz Biotechnology Cat# SC-200757 Fludarabine (STAT1 inhibitor) Selleckchem Cat# S1491 79-6 (BCL6 inhibitor) Selleckchem Cat# S8250 Bortezomib EMD Millipore Cat# 504314 LPS Sigma Cat# L2630 Hoechst 33258 ThermoFisher Cat# H3569 Normal mouse serum Jackson ImmunoResearch Cat# 015-000-120 penicillin-streptomycin Gibco Cat# 10378016 phorbol 12-myristate 13-acetate (PMA) Sigma Cat# P8139 Ionomycin Sigma Cat# I3909 Brefeldin A Biolegend Cat# 420601 Critical commercial assays Thiazolyl Blue Tetrazolium Bromide (MTT) Millipore-Sigma Cat# M5655 DuolinkTM flowPLA Detection Kit Millipore-Sigma Cat# DUO94001 DuolinkTM In Situ PLA Probe Anti-Rabbit PLUS Millipore-Sigma Cat# DUO92002 DuolinkTM In Situ PLA Probe Anti-Mouse MINUS Millipore-Sigma Cat# DUO92004 DuolinkTM In Situ Wash Buffer Millipore-Sigma Cat# DUO82047 IgG Mouse ELISA Kit ThermoFisher Cat# 8850-40022 IgM Mouse ELISA Kit ThermoFisher Cat# 8850-47022 Tissue Extraction Reagent ThermoFisher Cat# FNN0071 LDH-Glo cytotoxicity assay kit Promega Cat# J2380 (Continued on next page) e3 Cell Reports Medicine 3, 100744, September 20, 2022



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    Figure 5 ｜LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of <t>STAT3,</t> pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by <t>STA21</t> (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.
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    Figure 5 ｜LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of <t>STAT3,</t> pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by <t>STA21</t> (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.
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    Image Search Results


    Figure 5 ｜LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of STAT3, pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by STA21 (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.

    Journal: Neural regeneration research

    Article Title: Microglial depletion impairs glial scar formation and aggravates inflammation partly by inhibiting STAT3 phosphorylation in astrocytes after spinal cord injury.

    doi: 10.4103/1673-5374.357912

    Figure Lengend Snippet: Figure 5 ｜LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of STAT3, pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by STA21 (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.

    Article Snippet: To assess the role of STAT3 signaling, astrocytes were pretreated with 10 μM STA21 (an inhibitor of STAT3, Selleck, Shanghai, China, Cat# S7951) for 72 hours before coculture.

    Techniques: In Vitro, Staining, Western Blot, Incubation, Immunofluorescence