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Biomol GmbH
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Cayman Chemical
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Sapphire North America
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Enzo Biochem
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Verlag GmbH
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Cedarlane
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Bertin Pharma
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Iiyama Corporation
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Charlesson
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Image Search Results
Journal: PLoS ONE
Article Title: Interleukin-10 Inhibits Lipopolysaccharide Induced miR-155 Precursor Stability and Maturation
doi: 10.1371/journal.pone.0071336
Figure Lengend Snippet: (A) RAW264.7 cells were transfected with the c-fos promoter reporter and TK-Renilla, and were pretreated with DMSO or 30 µM STA-21 for 1 hour prior to IL-10 stimulation for 6 hours. Luciferase activity was measured and plotted as firefly/renilla ratio. (B) SCRMB and SHIP1 siRNA transduced cells were treated as except the cells were pretreated with DMSO or 30 µM STA-21 for 1 hour prior to stimulation. Expression levels of pri-miR-155 at 2 hours and miR-155 at 4 hours were measured by real time PCR and plotted relative to the LPS alone samples. Statistical significance between stimulation conditions was calculated by a two-way ANOVA test with a 95% confidence (** p <0.01, *** p <0.001, **** p <0.0001). Results were observed in at least two independent experiments.
Article Snippet: AQX-MN100 (Aquinox Pharmaceuticals, Vancouver, BC) was dissolved in ethanol, and
Techniques: Transfection, Luciferase, Activity Assay, Expressing, Real-time Polymerase Chain Reaction