anti sqor  (Proteintech)

Journal: The Journal of Biological Chemistry

Article Title: Hydrogen sulfide–dependent activation of human sulfide quinone oxidoreductase

doi: 10.1016/j.jbc.2025.110681

Figure Lengend Snippet: Effect of sulfurtransferase knockdowns on SQOR activity . A – B , Western blot analysis of MPST ( A ) and TST ( B ) in control (HT-29 and OR7G3) versus the corresponding CRISPR knockdown cell lines. Ponceau staining is shown as equal loading controls. C , representative Western blot of SQOR in control HT-29 cells ( lane 1) compared with HT-29 cells with CRISPR knockdown of OR7G3 ( lane 2), MPST ( lane 3), and TST ( lane 4). The image was spliced at the position indicated by the dashed line to remove a lane that is not relevant to this study. D , SQOR activity in HT-29 cells with the indicated CRISPR knockdowns. Each data point represents an independent experiment and is the mean ± SD of at least two technical replicates. Significance was determined using a two-tailed t test. MPST, mercaptopyruvate sulfurtransferase; OR7G3, olfactory receptor 7G3; SQOR, sulfide quinone oxidoreductase; TST, thiosulfate sulfurtransferase.

Article Snippet: The SQOR (17256-1-AP; Proteintech), TST (66018-1-Ig; Proteintech Group), and MPST (Novus Biologicals; NBP154734) antibodies were purchased from the indicated vendors.

Techniques: Activity Assay, Western Blot, Control, CRISPR, Knockdown, Staining, Two Tailed Test

Journal: The Journal of Biological Chemistry

Article Title: Hydrogen sulfide–dependent activation of human sulfide quinone oxidoreductase

doi: 10.1016/j.jbc.2025.110681

Figure Lengend Snippet: Effect of cystine, PPG, and BSO on SQOR activity in HT-29 cells with MPST and TST knockdowns . A , SQOR activity in cells grown in the presence of cystine (750 μM final concentration) for 24 h. B – C , SQOR activity in cells treated for 96 h with 2.5 mM PPG ( B ) or 20 μM BSO ( C ). Each data point represents an independent experiment and is the mean ± SD of at least two technical replicates. Significance was determined using the two-tailed t test. BSO, buthionine sulfoximine; MPST, mercaptopyruvate sulfurtransferase; PPG, ropargylglycine; SQOR, sulfide quinone oxidoreductase; TST, thiosulfate sulfurtransferase.

Article Snippet: The SQOR (17256-1-AP; Proteintech), TST (66018-1-Ig; Proteintech Group), and MPST (Novus Biologicals; NBP154734) antibodies were purchased from the indicated vendors.

Techniques: Activity Assay, Concentration Assay, Two Tailed Test

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: DSS‐induced acute UC significantly reduced SQOR levels. (A) C57BL/6 mice were treated with 3% DSS or water for 7 days, representative immunohistochemical (IHC) staining of SQOR in the colon, red arrows indicated intestinal epithelial cells (scale bar: 200 µm; enlarged scale bar: 100 µm; n = 3). (B, C) Mice were treated with 3% DSS for 7 days, and then colon was prepared to detect SQOR expression using western blot ( n = 4). (D, E) Mice were treated with 3% DSS for 3, 5, and 7 days, representative photograph of colon tissue during DSS‐induced acute UC in colon tissue of WT mice, and the colon length was recorded at days 0, 3, 5, 7 ( n = 3). (F, G) Relative SQOR protein expression in colonic tissues from controls or DSS‐treated WT mice during days 0, 3, 5, 7 using western blot ( n = 3). The data were represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significant difference.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: SQOR deficiency in the intestinal epithelial cells exacerbates DSS‐induced UC. (A) Western blot analysis of SQOR level in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice ( n = 3). (B) Immunofluorescence analysis of SQOR (green), Villin (red), and DAPI (blue) in the mouse colonic section was determined by immunofluorescence staining (scale bar: 100 µm; n = 3). (C, D) Daily body weight changes and DAI of Sqor FL/FL and SQOR CKO mice treated with 3% DSS. (E, F) A representative photograph of colon of Sqor FL/FL and Sqor CKO mice on day 7 after DSS treated or not, and the colon length was recorded. (G, H) The histological analysis of colon sections was performed H&E staining from Sqor FL/FL and SQOR CKO mice after DSS treated or not (scale bar: 200 µm; enlarged scale bar: 100 µm), histological scores from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 3). (I, J) Apoptotic cells in colonic sections as determined by TUNEL assay (scale bar: 200 µm; n = 3). (K) Cytokines and chemokines mRNA levels in colon tissues from Sqor FL/FL and Sqor CKO mice after DSS treated or not. The data were represented as mean ± SD. n = 6 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, no significant difference.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques: Western Blot, Immunofluorescence, Staining, TUNEL Assay

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: SQOR deficiency reduces intestinal barrier function in DSS‐induced acute UC in mice. (A) FITC‐dextran of serum determined intestinal permeability from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 4). (B) Representative TEM images of tight junctions between intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treatment or not, white arrows indicating tight junctions. (C, D) The occludin in the mouse colon sections was determined by immunofluorescence staining from Sqor FL/FL and Sqor CKO mice after DSS treated or not (scale bar: 200 µm). (E, F) The ZO‐1 in the mouse colon sections was determined by immunofluorescence staining from Sqor FL/FL and Sqor CKO mice after DSS treated or not (scale bar: 200 µm). The data were represented as mean ± SD. n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significant difference.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques: Permeability, Immunofluorescence, Staining

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: SQOR deficiency drives mitochondrial damage in intestinal epithelial cells. (A) Representative mitochondria images of DSS‐stimulated intestinal epithelial cells by TEM from Sqor FL/FL and Sqor CKO mice after DSS treated or not, red arrows indicate damaged mitochondria (scale bar: 5 µm; enlarged scale bar: 500 nm; n = 3). (B) Percentage of damaged mitochondria ( n = 3). (C) Detection of the mRNA levels of Atp5a1, Cox4i1, Uqcrc1, and Ndufab1 in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 5). (D) The mtDNA copy number in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 6). (E) The ATP level in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 4). The data were represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, no significant difference.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques:

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: SQOR maintains mitochondrial dynamics homeostasis. (A) Mito‐Tracker was employed to mark mitochondria in NCM460 cells with transfected siRNA‐ SQOR or NC siRNA in the presence or absence of DSS (scale bar: 20 µm; enlarged scale bar: 5 µm). (B) Representative TEM of mitochondria images in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not, red lines represent mitochondrial length measurements. (C) Quantitative analysis of mitochondrial length in TEM images. (D, E) Western blot analysis of SQOR and DRP1 in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not. (F, G) The DRP1 in the mouse colon sections was determined by immunofluorescence staining from Sqor FL/FL and Sqor CKO mice after DSS treated or not (scale bar: 100 µm). The data were represented as mean ± SD. n = 3 per group. *p < 0.05, **p < 0.01. ns, no significant difference.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques: Transfection, Western Blot, Immunofluorescence, Staining

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: Intestinal epithelial cell function is associated with ROS. (A) Detection of ROS level in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 4). (B) GSH/GSSG ratio in intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 6). (C) The mRNA levels of PGC1α, Nrf1, and Tfam in mouse intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 6). (D) The mRNA levels of Gpx, Trx2, and Sod2 in mouse intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 4). (E) The mRNA levels of Ucp2, Ucp4, and Ucp5 in mouse intestinal epithelial cells from Sqor FL/FL and Sqor CKO mice after DSS treated or not ( n = 3). The data were represented as mean ± SD. n = 6 per group. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques: Cell Function Assay

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: NAC alleviates DSS‐induced colitis model. (A) Daily DAI of Sqor FL/FL and Sqor CKO colitis mice treated with NAC. (B, C) A representative photograph of colon of Sqor FL/FL and Sqor CKO mice colitis mice treated with NAC, and the colon length was recorded. (D, E) The histological analysis of colon sections was performed H&E staining from Sqor FL/FL and Sqor CKO mice treated with NAC (scale bar: 100 µm), histological scores from Sqor FL/FL and Sqor CKO mice treated with NAC ( n = 3). (F, G) Cytokines and chemokines mRNA levels in colon tissues from Sqor FL/FL and Sqor CKO mice treated with NAC ( n = 3). The data were represented as mean ± SD. n = 6 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, no significant difference.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques: Staining

Journal: MedComm

Article Title: Sulfide Quinone Oxidoreductase Alleviates Acute Ulcerative Colitis by Regulating Mitochondrial Dysfunction

doi: 10.1002/mco2.70285

Figure Lengend Snippet: A proposed mechanism by which SQOR alleviated DSS‐induced acute UC by ameliorating mitochondrial dysfunction.

Article Snippet: SiRNA‐ SQOR and negative control (NC) siRNA were purchased from Genepharma (Shanghai, China).

Techniques:

Journal: Communications Medicine

Article Title: The treatment of primary CoQ deficiency requires the targeting of multiple pathogenic mechanisms

doi: 10.1038/s43856-025-01000-8

Figure Lengend Snippet: Representative image of Western blot of SQOR and the quantitation of the protein bands ( a ), and relative mRNA levels for Sqor ( b ) in the liver. c Representative image of Western blot of Eif2ak2 and the quantitation of the protein bands in the liver. Relative mRNA levels of the genes Cmpk2 ( d ), Mthfd2l ( e ), Psat1 ( f ) and Eif2ak2 ( g ) in liver. Data are expressed as mean ± SD (one-way ANOVA with a Tukey’s post hoc test; n = 5 for each group). Representative images of Western blots of SQOR ( h ), CBS ( i ), PSAT1 ( j ) and EIF2AK2 ( k ), and the quantitation of the protein bands in human skin fibroblast from control and patient 1 (P1). Relative mRNA levels of the genes CMPK2 ( l ), MTHFD2L ( m ), PSAT1 ( n ) and EIF2AK2 ( o ) in human skin fibroblast from control and patient 1 (P1). Representative images of Western blots of SQOR ( p ), CBS ( q ), PSAT1 ( r ) and EIF2AK2 ( s ), and the quantitation of the protein bands in human skin fibroblast from control and patient 2 (P2). Relative mRNA levels of the genes CMPK2 ( t ), MTHFD2L ( u ), PSAT1 ( v ) and EIF2AK2 ( w ) in in human skin fibroblast from control and patient 2 (P2). Data are expressed as mean ± SD (one-way ANOVA with a Tukey’s post hoc test; n = 3 for each group). CBS cystathionine β-synthase, CMPK2 cytidine monophosphate kinase 2, EIF2AK2 eukaryotic translation initiation factor 2 alpha kinase 2, MTHFD2L methylenetetrahydrofolate dehydrogenase (NADP + Dependent) 2-Like, PSAT1 phosphoserine aminotransferase 1, SQOR sulfide:quinone oxidoreductase. Original western-blot membranes corresponding to this figure are provided in Figs. S8 and Fig S9.

Article Snippet: The following primary antibodies were used: anti-COQ4 (Proteintech, 16654-1-AP), anti-COQ5 (Proteintech, 17453-AP), anti-COQ7 (Proteintech, 15083-1-AP), anti-PRODH (Cell Signaling, #22980), anti- SQRDL (Proteintech, 17256-1-AP), anti-CBS (Proteintech, 14787-1-AP), anti-EIF2AK2 (Proteintech, 18244-1-AP), anti-PSAT1 (Proteintech, 10501-1-AP), anti-VDAC1 (Abcam, ab14734) and anti-ACTIN (Invitrogen, #MA5-15739-HRP).

Techniques: Western Blot, Quantitation Assay, Control