slc27a2  (Proteintech)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Autophagic regulation of CD8 + T cell metabolic reprogramming defines acute and latent phases of cytomegalovirus infection in vivo

doi: 10.3389/fcimb.2025.1673229

Figure Lengend Snippet: Dynamic alteration of metabolism in CD8 + T cells following CMV infection. (A) mRNA levels of ENTPD1 and SLC27A2 (metabolic markers) in splenocytes increased at early time points (week 3, 4) and declined at week 6 during CMV infection (n=3). (B) Immunofluorescent staining revealed that CD8 + T cell (red color) in infected rat spleen showed co-expression (merge, arrowheads) of SLC27A2 (green color) at 3w and 4w (n=3). Scale bars: 10 μm. Data shown are representative of 3 independent experiments. (C) Expression levels of ENTPD1 and SLC27A2 in splenocytes were modulated by autophagy enhancer (Rapamycin) and inhibitors (Chloroquine and Bafilomycin) in vitro (n=3). Error bars indicate SEM; * p < 0.05, ** p < 0.01.

Article Snippet: The immunofluorescent double staining of CD8 (#200610, Biolegend) and SLC27A2 (#14048-1-AP, Proteintech, Chicago, IL, USA); on formalin-fixed paraffin sections was performed as described previously ( ).

Techniques: Infection, Staining, Expressing, In Vitro

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: Representative IHC images of pancreatic islets from db/db ( A ) and FATP2-KO db/db ( B ) mice. As described in Methods, α and β cells were labeled with glucagon and insulin antibodies, respectively. Scale bars: 100 μm. ( C ) β Cell mass was calculated as described in Methods for db/db and FATP2-KO db/db mice. Data represent the mean ± SEM. * P < 0.01, by Student’s t test.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Labeling

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: WT mouse pancreatic islets were immunohistochemically labeled for FATP2 expression in α, β, and δ cells as described in Methods. Merged images, representing cell-specific FATP2 expression, are shown in yellow. Micrometer scale bars are shown at the bottom right of each merged image.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Labeling, Expressing

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A ) Paraffin sections of human pancreas were immunohistochemically labeled for FATP2 expression in α and β cells as described in Methods. Merged images, representing cell-specific FATP2 expression, are shown in yellow. Micrometer scale bars are shown at the bottom of each image. ( B ) Gene expression correlation between GCG and FATP2 gene ( SLC27A2 ) from 2 public normal human islet transcriptome datasets ( GSE38642 and GSE50397 ) using online software ( http://r2.amc.nl ). Data were analyzed by linear regression and Pearson correlation.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Labeling, Expressing, Gene Expression, Software

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A and B ) FATP2 and loading control GAPDH mRNA expression was determined in human and mouse pancreatic tissue and α cell lines by RT-PCR (as described in Methods). Data are representative of 3 experiments per condition. ( C ) Mouse αTC1-6 cells were preincubated with lipofermata (1 hour, 37°C, 0–50 μM) in triplicate. BODIPY-labeled fatty acid uptake velocity was then determined, as described in Methods. Results show the mean ± SEM of 4 experiments.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Labeling

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A ) Random (nonfasting) plasma glucagon concentrations in 4- to 6-month-old WT and db/db mice with or without FATP2 gene deletion. Each symbol in the scatter bars represents the mean from 1 sample assayed in duplicate ( n = glucagon concentrations from 9–17 mice per genotype). * P < 0.05 compared with WT by ANOVA with Tukey’s post hoc test for multiple comparisons. ( B ) Serial glucose measurements in 4- to 6-month-old WT and db/db mice with or without FATP2 gene deletion ( n = 3 mice per group) following alanine administration (2 g/kg i.p.). Data represent the mean ± SEM.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Clinical Proteomics

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A ) OGTTs and IPGTTs were conducted in db/db and FATP2-KO db/db mice, as described in Methods. Blood glucose levels were determined at the indicated times in 5 mice per group. ( B ) As an index of glucose disposal, the AUC corresponding to FATP2-KO db/db experiments in A was integrated using GraphPad Prism 7 software. ( C ) Plasma was obtained at baseline and at the 1-hour time point during the OGTTs or IPGTTs in FATP2-KO db/db mice. Data represent the mean ± SEM.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Software, Clinical Proteomics

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: Slc27a2 ( A ) and Gcg ( B ) mRNA expression levels were determined in mouse gut segments by qPCR, as described in Methods. Data were normalized to expression in stomach, which was defined as 1.0. Immunohistochemical labeling of FATP2 and GLP-1 in human distal ileum ( C and D ) (note that FATP2 is red and GLP-1 is green) and duodenum ( E and F ) (note that FATP2 is green and GLP-1 is red). Representative images from 5 mice are shown. Original magnification, ×200 ( C and E ) and ×600 ( D and F ). Data represent the mean ± SEM.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Expressing, Immunohistochemical staining, Labeling

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A ) Pancreatic GLP-1 + α cell mass was determined as described in Methods in db/db and FATP2-KO db/db mice. * P < 0.01 compared with the db/db group by t test. ( B ) Human islets were preincubated with or without lipofermata (LF) and then tested for glucose-stimulated GLP-1 secretion as described in Methods. * P < 0.01 compared with all other groups by ANOVA. ( C ) αTC1-6 cells were preincubated with or without lipofermata or palmitate (Palm) as indicated. Glucose-stimulated GLP-1 secretion was then measured as described in Methods. Each symbol in the scatter bars in B and C represents 1 sample that was assayed in duplicate ( n = 3–6 samples per condition). * P < 0.05 compared lipofermata plus palmitate by ANOVA. ( D ) αTC1-6 cells coincubated in 5 mM or 25 mM glucose with or without 400 μM palmitate with or without 50 μM lipofermata for 16 hours were analyzed for Pcsk1 and Pcsk2 mRNA expression by qPCR. The results are expressed as the ratio relative to the 5 mM glucose-only condition. * P < 0.05 compared with other groups by ANOVA. ( E ) Glucose-stimulated insulin secretion was measured in human islets, which were preincubated with or without lipofermata and then exposed to exendin[9-39] (Ex) or palmitate, as described in Methods. Each symbol in the scatter bars represents 1 sample that was assayed in duplicate ( n = 3 samples per condition). * P < 0.01 compared with 16.8 mM glucose plus the lipofermata group by ANOVA. Data represent the mean ± SEM.

Article Snippet: Conditional proximal tubule FATP2-KO mice were generated from intercrosses between GGT1 -Cre (The Jackson Laboratory) and Slc27a2 -floxed mice (gift from Dmitry Gabrilovich, The Wistar Institute, Philadelphia, Pennsylvania, USA [ref. ]) on a congenic C57BL-KS/J background.

Techniques: Expressing

Journal: Nature

Article Title: ACLY inhibition promotes tumour immunity and suppresses liver cancer

doi: 10.1038/s41586-025-09297-0

Figure Lengend Snippet: a , Overlay of the extracted ion chromatograms for EVT0185 reaction mixture at 0 and 30 min. b , Mass spectrum of EVT0185-CoA at 30-min. c , Fragment ions of EVT0185-CoA at 30-min sample showing the fragment ion 605 m/z produced following the characteristic neutral loss of 3’-phosphonucleoside diphosphate (507 Da). Western blot showing d , Dkk tag expression in empty vector control (pCMV), SLC27A1 , SLC27A2 , SLC27A4 , and SLC27A5 transfected HEK293 cells e , SLC27A2 (ACSVL1) protein expression in non-transfected cells (lane 1), pCMV controls (lanes 2–4) and SLC27A2 (lanes 5–7) transfected cells; β-actin as a loading control. For gel source data, see Supplementary Fig. . f , Extracted ion chromatogram from the MS1 and MS2 scans of HEK293 cells transfected with SLC27A2 and treated with 30 µM EVT0185. g , Mass spectrum from the MS1 and MS2 scans which shows the parent ion (1112 m/z) and the neutral loss of 3’-phosphonucleoside diphosphate (507 Da), respectively. h , EVT0185-CoA detected only in extracts from HEK293 cells overexpressing SLC27A2 (ACSVL1); mean ± SEM, n = 6 biologically independent samples/group. i , Proportion of human liver cells expressing SLC27A2 mRNA at different stages of disease progression. j , Human SLC27A2 protein expression in different cell types. k , Flow cytometry gating strategy: lymphocyte population identified via FSC vs. SSC, singlets via FSC-A vs. FSC-H, live cells via FSC vs. e780 viability dye, and CD19 + B cells and CD3 + T cells via CD19 vs. CD3 plot. l , PBMCs stimulated with R848 and IL-2 showing CD19 + B cell proliferation compared to negative control. m , Average proliferation index of R848-induced CD19 + B cells with EVT0185 or vehicle, n = 2 biologically independent PBMC donors. n , PBMCs stimulated with Concanavalin A (Con A) and IL-2 showing CD3 + T cell proliferation compared to negative control. o , Average proliferation index of Con A-induced CD3 + T cells with EVT0185 or vehicle, n = 2 biologically independent PBMC donors.

Article Snippet: HEK293 cells were transfected with expression plasmid for SLC27A1 ( NM_198580 , human tagged ORF clone; RC209285, OriGene), SLC27A2 ( NM_003645 , human tagged ORF clone; RC221033, OriGene), SLC27A4 ( NM_005094 , human tagged ORF clone; RC209557, OriGene), SLC27A5 ( NM_012254 , human tagged ORF clone; RC215758, Origene) or control vector (pCMV6-entry mammalian expression vector; PS100001, OriGene).

Techniques: Produced, Western Blot, Expressing, Plasmid Preparation, Control, Transfection, Biomarker Discovery, Flow Cytometry, Negative Control