Journal: bioRxiv
Article Title: A Sap Peptide Conserved across Flowering Plants Positively Regulates Lignin Biosynthesis, Biomass and Immunity
doi: 10.1101/2024.05.20.594799
Figure Lengend Snippet: The changes of monolignol metabolites on (A) the debarked stems of Populus trichocarpa and (B) the stems of Eucalyptus grandis with different concentrations (0.1 μM, 0.5 μM and 1 μM) of ASAP treatment after one month, and (C) the leaves and (D) the stems of Solanum lycopersicum (tomato) with 1 μM ASAP treatment after 24 hours. Ten monolignol metabolites were quantified using LC-SRM-MS analysis. Metabolites in the main fluxes of H-lignin, G-lignin and S-lignin biosynthesis are represented. The detected compounds were highlighted, pink for significant up-regulation, dark grey for no significant change, light grey for no detection. C-Aci, caffeic acid; G-Aci, ferulic acid; 5H-Aci, 5-hydroxyferulic acid; S-Aci, sinapic acid; G-Ald, coniferaldehyde; 5H-Ald, 5-hydroxyconiferaldehyde; S-Ald, sinapaldehyde; H-Alc, 4-coumaryl alcohol; G-Alc, coniferyl alcohol; S-Alc, sinapyl alcohol. One and two asterisks represent Student’s t -test p < 0.05 and 0.01, respectively. Three individual plants were performed as biological replicates for each treatment.
Article Snippet: Nine standards were purchased, including caffeic acid (98%, AK Scientific), ferulic acid (99%, Acros Organics), 4-coumaryl alcohol (98%, Toronto Research Chemicals), coniferaldehyde (99.94%, MedChemExpress), coniferyl alcohol (97.5%, Supelco Merck), 5-hydroxyferulic acid (95%, Sigma-Aldrich Merck), sinapic acid (98%, AK Scientific), sinapaldehyde (99.96%, MedChemExpress) and sinapyl alcohol (95%, Biosynth).
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