sb431542 (Tocris)

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Tocris sb431542
Sb431542, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 2553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sb431542/SB+431542/pmc13091051-265-15-21
Average 97 stars, based on 2553 article reviews

sb431542 - by Bioz Stars, 2026-09

97/100 stars

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Cell Culture:

Article Title: Attenuated interferon signalling in alveolar epithelium limits resistance to Streptococcus pyogenes
Article Snippet: Cells were then dissociated using Gentle Cell Dissociation Reagent (StemCell Technologies, 100-0485) and replated on plates coated with growth factor-reduced Matrigel (Corning, 354277). .. Afterwards, cells were cultured in the anteriorisation media “DS/SB” (cSFDM with 2 μM Dorsomorphin (Tocris, 3093/10) and 10 μM SB431542 (Tocris, 1614)) for 3 days, with the first 24 hours also supplemented with 10 μM Y-27632 (Tocris, 1254). .. From day 6 of the differentiation, Cells were kept in “CBRa” (cSFDM with 3 μM CHIR99021 (Tocris, 4423), 10 ng/ml rhBMP4 (R&D Systems, 314-BP), and 100 nM Retinoic Acid (Sigma, R2625)) to induce NKX2-1 + lung progenitors.

Article Title: AMPK-p38 axis converts human pluripotent stem cells to naive state
Article Snippet: Finally, the medium was switched to RPMI1640 (Gibco)+B27 medium (RPMI1640, 2 mM L-glutamine, x1 B27), and cultured for 1 week. .. For ectoderm differentiation, re-primed cells were cultured with Ndiff227 (Takara Bio) supplemented with hbFGF (Fujifilm,10 ng/ml), SB431542 (Tocris, 20 μM), and Noggin (R&D, 260 ng/ml) for 4 days, and then the medium was switched to Ndiff227 (Takara Bio) supplemented with hbFGF (Fujifilm,10 ng/ml) and SB431542 (Tocris, 20 μM). ..

Knock-Out:

Article Title: Innervated organoid compositions and methods of making same
Article Snippet: Derivation of Neural Crest (NC) from hPSC Lines At day 0, Control or mutant hiPSCs were seeded on Matrigel-coated plate (105 cells cm-2) in iPS cell medium containing 10 ng ml-1 FGF2 (PeproTech, 100-18B) and 10 μM Y-27632. .. Differentiation was then initiated by replacing iPS cell medium with KSR medium, containing knockout DMEM plus 15% KSR (Life Technologies, 10828-028), NEAA (Life Technologies, 11140-050), L-glutamine (Life Technologies, 25030-081), β-mercaptoethanol (Life Technologies, 21985-023), LDN193189 (100 nM, Stemgent) and SB431542 (10 μM, Tocris). .. The dual SMAD inhibitors and a potent GSK inhibitor were added at different time frame during the NC induction, including LDN193189 (from day 0 to day 3), SB431542 (from day 0 to day 4), 3 μM CHIR99021 (from day 2 to day 10, Tocris Bioscience, 4423).

Article Title: Innervated organoid compositions and methods of making same
Article Snippet: Sections were photographed using a Carl Zeiss LSM780 or LSM810 confocal microscope. .. Media The NCC induction medium may contain knockout Dulbecco's Modified Eagle Medium (DMEM) plus 15% Knockout Serum Replacement (KSR) (Life Technologies, 10828-028), NEAA (Life Technologies, 11140-050), L-glutamine (Life Technologies, 25030-081), β-mercaptoethanol (Life Technologies, 21985-023), LDN193189 (100 nM, Stemgent) and SB431542 (10 μM, Tocris). .. The dual SMAD inhibitors and a potent GSK inhibitor may be added at different point in time during NC induction, such as, for example, including LDN193189 (from day 0 to day 3), SB431542 (from day 0 to day 4), 3 μM CHIR99021 (from day 2 to day 10, Tocris Bioscience, 4423).

In Vitro:

Article Title: Transcriptomic and phenotypic convergence of neurodevelopmental disorder risk genes in vitro and in vivo.
Article Snippet: .. At 0 days in vitro (DIV0), 3182-3-clone5 hiPSCs were dissociated and plated at 1.5 × 106 cells per well onto Geltrex-coated six-well plates (1:250 dilution coating) in SNaP Induction Media (DIV0): DMEM/ F12 with Glutamax (ThermoFisher, cat. no. 11320082), Glucose (0.3% v/v), N2 Supplement (1:100, ThermoFisher, cat. no. 17502048), doxycycline (2 μg ml−1; Sigma-Aldrich, cat. no. D9891), LDN-193189 (200 nM; Stemgent, cat. no. 04-0074), SB431542 (10 μM; Tocris, cat. no. 1614) and XAV939 (2 μM; Stemgent, cat. no. 04-00046) supplemented with 25 ng ml−1 Chroma I ROCK2 Inhibitor. .. After 24 h, DIV2, cells were fed with Selection Media: DMEM/F12 with Glutamax, Glucose (0.3% v/v), N2 Supplement (1:100), doxycycline (2 μg ml−1), Geneticin (0.5 mg ml−1; Thermofisher, cat. no. 10131035), LDN-193189 (100 nM), SB431542 (5 μM) and XAV939 (1 μM).

Modification:

other:

Article Title: AAV immuno-gene therapy platform delivering vectorized cytokines defines a new modality for high-grade glioma treatment
Article Snippet: 24, 48, 72, and 96 h after treatment, cell supernatants were collected and IFN variant levels were measured using IFN ELISA kits following the manufacturer’s instructions (human IFNα [PBL Cat# 41135-1], human IFNβ [PBL Cat#41410], and human IFNγ [R&D Systems Cat#: DIF50C]).

Review