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sal003  (MedChemExpress)


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    Structured Review

    MedChemExpress sal003
    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
    Sal003, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sal003/product/MedChemExpress
    Average 93 stars, based on 6 article reviews
    sal003 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "The Integrated Stress Response Pathway Improves Aged Murine Muscle Stem Cell Activation and in vivo Regeneration"

    Article Title: The Integrated Stress Response Pathway Improves Aged Murine Muscle Stem Cell Activation and in vivo Regeneration

    Journal: bioRxiv

    doi: 10.1101/2025.09.26.678822

    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM Sal003 (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
    Figure Legend Snippet: A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM Sal003 (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.

    Techniques Used: In Vitro, Cell Culture, shRNA



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    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
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    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
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    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
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    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
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    Image Search Results


    A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM Sal003 (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.

    Journal: bioRxiv

    Article Title: The Integrated Stress Response Pathway Improves Aged Murine Muscle Stem Cell Activation and in vivo Regeneration

    doi: 10.1101/2025.09.26.678822

    Figure Lengend Snippet: A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM Sal003 (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.

    Article Snippet: Adult (4 month) and aged (20-22 month) C57 mice were administered 1-hour pre-BaCl 2 injury via IP either Sal003 (HY-15969, MedChemExpress, 1 mg/kg) or ISRIB (HY-12459, MedChemExpress, 1 mg/kg), both dissolved in DMSO and PBS.

    Techniques: In Vitro, Cell Culture, shRNA