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rpmi 1640 medium  (ATCC)


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    Structured Review

    ATCC rpmi 1640 medium
    Rpmi 1640 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 19636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rpmi/RPMI-1640+Medium/pm42826497-52-9-1
    Average 99 stars, based on 19636 article reviews
    rpmi 1640 medium - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: A suture-specific oocyst wall protein COWP4 is essential for excystation and infectivity, while COWP6 links wall architecture to host interaction in Cryptosporidium parvum
    Article Snippet: .. For in vitro culture, HCT-8 cells (ATCC #CCL-244) were maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/mL), and streptomycin (100 μg/mL) at 37 °C with 5% CO2. ..

    Cell Culture:

    Article Title: Bispecific targeting of CHI3L1 and PD-1 as a therapeutic strategy for pulmonary fibrosis.
    Article Snippet: .. Human monocytic THP-1 cells (ATCC, TIB-202) were cultured in RPMI-1640 supplemented with 10% heat-inactivated FBS and antibiotics (1% penicillin–streptomycin). .. Normal human lung fibroblasts (NHLFs, ATCC) were cultured on FBM® Fibroblast Growth Basal Medium with FGM® -2 Fibroblast Growth Medium-2 SingleQuots® Supplements and Growth Factors.

    Article Title: Structure-Based Discovery of Potent BCL-XL Inhibitors through Rescaffolding.
    Article Snippet: .. MOLT-4 cells (ATCC No. CRL-1582) were cultured in RPMI supplemented with 10% FBS. .. Cells were resuspended in 50% matrigel (BD Biosciences) and 0.1 mL containing 5 × 106 cells was subcutaneously inoculated into the right flank of female NOD SCID mice (Charles River).

    Article Title: Modulation of tumor-derived exosomes and reprogramming of cancer-associated fibroblasts for colorectal cancer therapy.
    Article Snippet: .. Cell culture Human colorectal cancer cell lines HCT116 and LoVo were purchased from the American Type Culture Collection (ATCC) and routinely cultured in RPMI-1640 high-glucose or F-12K medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37°C with 5% CO2. ..

    Article Title: A phosphoproteome atlas of human cell lines reveals the landscape of kinase activity.
    Article Snippet: MV-4-11 (CRL-9591) and OCI-M1 (ACC 529) were cultured in IMDM (Gibco). .. SU-DHL-4 (CRL-2957) was cultured in RPMI with American Type Culture Collection (ATCC) modification (Gibco). .. MCF-10A (CRL-10317) was cultured in DMEM/F12 with glutaMAX (Gibco) supplemented with 5% horse serum (Gibco), 1% (wt/vol) nonessential amino acids (Gibco), 0.01 mg ml−1 insulin (Sigma-Aldrich), 100 ng ml−1 cholera toxin (Sigma-Aldrich), 500 ng ml−1 hydrocortison (Sigma-Aldrich) and 20 ng ml−1 EGF (Peprotech).

    Multiple Displacement Amplification:

    Article Title: Oxynitidine derivatives useful as inhibitors of topoisomerase IB (TOP1) and tyrosyl-DNA phosphodiesterase 1 (TDP1)
    Article Snippet: .. TABLE 8 Cytotoxicity of 67 and 50 against individual NCI-60 cell lines GI50 (μM)a GI50 (μM) Panel Cell line 67 50 Panel Cell line 67 50 MGMb 0.525 0.0977 Colon COLO 205 0.29 0.0605 Leukemia CCRF-CEM 0.15 0.0201 Cancer HCC-2998 1.02 0.156 K-562 0.161 0.0394 HCT116 0.285 0.0515 MOLT-4 0.0633 0.0234 HCT-15 0.42 0.116 RPMI-8226 0.257 0.0763 HT29 0.168 0.0508 SR 0.0907 0.0173 KM12 1.1 0.238 Non-Small A549/ATCC 0.584 0.0948 SW-620 0.243 0.0658 Cell EKVX 1.36 0.202 Renal 786-0 0.464 0.0703 Lung HOP-62 0.399 0.0439 Cancer A498 1.23 0.127 Cancer HOP-92 3.56 0.184 ACHN 0.366 0.0856 NCI-H226 1.34 0.172 CAKI-1 0.295 0.0644 NCI-H23 0.451 0.0906 RXF393 0.929 0.16 NCI-H322M 0.99 0.147 SN 12C 0.739 0.171 NCI-H460 0.23 0.0212 TK-10 1.35 0.316 NCI-H522 0.0875 0.0274 UO-31 0.35 0.104 CNS SF-268 0.442 0.0903 Breast MCF-7 0.164 0.0292 Cancer SF-295 0.279 0.0346 Cancer MDA-MB- 1.28 0.362 231/ATCC SF-539 1.06 0.124 HS 578T 1.96 0.904 SNB-19 0.398 0.116 BT-549 0.918 0.202 SNB-75 0.596 0.0885 T-47D 0.368 0.0757 U251 0.26 0.0394 MDA-MB-468 0.157 0.0599 Melanoma LOX IMVI 0.246 0.0389 Ovarian IGROV1 0.5 0.145 MALME-3M 0.613 0.174 Cancer OVCAR-3 0.826 0.166 M14 0.324 0.0581 OVCAR-4 0.782 0.154 MDA-MB- 0.759 0.118 OVCAR-5 1.2 0.183 435 SK-MEL-2 3.01 0.393 OVCAR-8 0.93 0.132 SK-MEL-28 3.05 0.573 NCI/ADR-RES 1.01 0.165 SK-MEL-5 0.614 0.0909 SK-OV-3 1.24 0.157 UACC-257 1.9 0.122 Prostate PC-3 0.532 0.112 UACC-62 0.278 0.0451 Cancer DU145 0.527 0.13 Regarding Table 8, the superscripts are defines as follows: aGI50 values were defined as the compound concentrations that resulted in 50% cell growth inhibition. ..

    Inhibition:

    Article Title: Oxynitidine derivatives useful as inhibitors of topoisomerase IB (TOP1) and tyrosyl-DNA phosphodiesterase 1 (TDP1)
    Article Snippet: .. TABLE 8 Cytotoxicity of 67 and 50 against individual NCI-60 cell lines GI50 (μM)a GI50 (μM) Panel Cell line 67 50 Panel Cell line 67 50 MGMb 0.525 0.0977 Colon COLO 205 0.29 0.0605 Leukemia CCRF-CEM 0.15 0.0201 Cancer HCC-2998 1.02 0.156 K-562 0.161 0.0394 HCT116 0.285 0.0515 MOLT-4 0.0633 0.0234 HCT-15 0.42 0.116 RPMI-8226 0.257 0.0763 HT29 0.168 0.0508 SR 0.0907 0.0173 KM12 1.1 0.238 Non-Small A549/ATCC 0.584 0.0948 SW-620 0.243 0.0658 Cell EKVX 1.36 0.202 Renal 786-0 0.464 0.0703 Lung HOP-62 0.399 0.0439 Cancer A498 1.23 0.127 Cancer HOP-92 3.56 0.184 ACHN 0.366 0.0856 NCI-H226 1.34 0.172 CAKI-1 0.295 0.0644 NCI-H23 0.451 0.0906 RXF393 0.929 0.16 NCI-H322M 0.99 0.147 SN 12C 0.739 0.171 NCI-H460 0.23 0.0212 TK-10 1.35 0.316 NCI-H522 0.0875 0.0274 UO-31 0.35 0.104 CNS SF-268 0.442 0.0903 Breast MCF-7 0.164 0.0292 Cancer SF-295 0.279 0.0346 Cancer MDA-MB- 1.28 0.362 231/ATCC SF-539 1.06 0.124 HS 578T 1.96 0.904 SNB-19 0.398 0.116 BT-549 0.918 0.202 SNB-75 0.596 0.0885 T-47D 0.368 0.0757 U251 0.26 0.0394 MDA-MB-468 0.157 0.0599 Melanoma LOX IMVI 0.246 0.0389 Ovarian IGROV1 0.5 0.145 MALME-3M 0.613 0.174 Cancer OVCAR-3 0.826 0.166 M14 0.324 0.0581 OVCAR-4 0.782 0.154 MDA-MB- 0.759 0.118 OVCAR-5 1.2 0.183 435 SK-MEL-2 3.01 0.393 OVCAR-8 0.93 0.132 SK-MEL-28 3.05 0.573 NCI/ADR-RES 1.01 0.165 SK-MEL-5 0.614 0.0909 SK-OV-3 1.24 0.157 UACC-257 1.9 0.122 Prostate PC-3 0.532 0.112 UACC-62 0.278 0.0451 Cancer DU145 0.527 0.13 Regarding Table 8, the superscripts are defines as follows: aGI50 values were defined as the compound concentrations that resulted in 50% cell growth inhibition. ..

    Modification:

    Article Title: Dual Pathways of Extracellular ATP Action in Cancer Cells: Purinergic Signaling–Driven Senescence and Macropinocytic ATP Internalization
    Article Snippet: .. Dulbecco’s Modified eagle Medium (DMEM) high glucose (ATCC-30-2002), Eagle’s Minimum Essential Medium (EMEM-ATCC 30-2003), RPMI (ATCC-30-2001), F12 (ATCC-30-2007), Ham’s F12 modified (ATCC-30-2004), McCoy’s 5A medium (ATCC-30-2007), 0.25% Trypsin-EDTA Solution, 1X (ATCC-30-2101), Fetal bovine serum (ATCC-30-2020), and Penicillin-Streptomycin Solution (ATCC-30-2300) were purchased from ATCC. .. Adenosine 5-triphosphate disodium salt hydrate (A2383), Resazurin sodium salt (R7017), 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) (A3085), IPA3 (I2285), Navitoclax (AMBH2D6F59B5) and 200 mM glutamine (59202C) were purchased from Sigma Aldrich.

    Article Title: A phosphoproteome atlas of human cell lines reveals the landscape of kinase activity.
    Article Snippet: MV-4-11 (CRL-9591) and OCI-M1 (ACC 529) were cultured in IMDM (Gibco). .. SU-DHL-4 (CRL-2957) was cultured in RPMI with American Type Culture Collection (ATCC) modification (Gibco). .. MCF-10A (CRL-10317) was cultured in DMEM/F12 with glutaMAX (Gibco) supplemented with 5% horse serum (Gibco), 1% (wt/vol) nonessential amino acids (Gibco), 0.01 mg ml−1 insulin (Sigma-Aldrich), 100 ng ml−1 cholera toxin (Sigma-Aldrich), 500 ng ml−1 hydrocortison (Sigma-Aldrich) and 20 ng ml−1 EGF (Peprotech).



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