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recombinant human granulocyte colony-stimulating factor (rhu g-csf; filgrastim)  (Amgen)

 
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    Amgen recombinant human granulocyte colony-stimulating factor (rhu g-csf; filgrastim)
    Recombinant Human Granulocyte Colony Stimulating Factor (Rhu G Csf; Filgrastim), supplied by Amgen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhu/recombinant+human+g+csf/pm40074513-40-15-18
    Average 90 stars, based on 1 article reviews
    recombinant human granulocyte colony-stimulating factor (rhu g-csf; filgrastim) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Real World Clinical Experience of Biosimilar G-CSF (Grastofil) for Autologous Peripheral Blood Stem Cell Mobilization: Single Center Experience in Canada Following Early Adoption
    Article Snippet: Granulocyte colony-stimulating factor (G-CSF) is the first line treatment for mobilization, most commonly using a regimen of daily filgrastim (rHu-G-CSF, originator brand Neupogen ® , AMGEN, Thousand Oaks, CA, USA) [ , ].

    Article Title: Lentiviral triplex DNA, and vectors and recombinant cells containing lentiviral triplex DNA
    Article Snippet: Clonogenic and Long Term Culture (LTC) Assays Clonogenic progenitors from human fresh CB cells were assayed in 0.8% methylcellulose containing 30% FCS, 1% deionized BSA, and 10-4M 2-mercaptoethanol, in the presence of 50 ng/mlrhu-SCF, 10 ng/ml rhu-GCSF (Amgen), 2 ng/ml rhu-IL3, and 2U/ml rhu-EPO (Amersham).

    Article Title: In Vivo Mobilization and Functional Characterization of Non-Human Primate Monocytic Myeloid-Derived Suppressor Cells
    Article Snippet: Prior to leukapheresis, monkeys received recombinant human (rhu) GM-CSF (Leukine; Genzyme, Cambridge, MA; 10μg/kg/day for 4 days), followed by rhu G-CSF (Neupogen; Amgen, Thousand Oaks, CA; 10μg/kg/day for 4 days).

    Article Title: Preliminary assessment of the feasibility of autologous myeloid-derived suppressor cell infusion in non-human primate kidney transplantation
    Article Snippet: Prior to leukapheresis, monkeys received recombinant human (rhu) granulocyte-macrophage (GM)-colony-stimulating factor (CSF) (Leukine; Genzyme, Cambridge, MA; 10μg/kg/day for 4 days), followed by rhu granulocyte (G)-CSF (Neupogen; Amgen, Thousand Oaks, CA; 10μg/kg/day for 4 days).

    Article Title: Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4°C
    Article Snippet: 14 Human CB HPC by colony assay Immediately following one hour acclimation to RT or 4°C, 5×10 4 human CB mononuclear cells were plated in 1 ml of colony assay media containing Iscove’s Modified Dulbecco’s Medium (IMDM, Lonza), 1% methylcellulose, 30% fetal bovine serum (FBS, Hyclone), 50 ng recombinant human (rhu) stem cell factor (SCF, R&D Systems), 10 ng rhu granulocyte-macrophage-colony stimulating factor (GM-CSF, R&D Systems), 10 ng rhu interleukin 3 (IL-3, R&D Systems), 1 unit rhu erythropoietin (EPO, Amgen) in a 37°C 5% CO 2 /O 2 environment.

    Article Title: Preliminary assessment of the feasibility of autologous myeloid-derived suppressor cell infusion in non-human primate kidney transplantation
    Article Snippet: Hematopoietic growth factor mobilization and leukapheresis Prior to leukapheresis, monkeys received recombinant human (rhu) granulocyte-macrophage (GM)-colony-stimulating factor (CSF) (Leukine; Genzyme, Cambridge, MA; 10μg/kg/day for 4 days), followed by rhu granulocyte (G)-CSF (Neupogen; Amgen, Thousand Oaks, CA; 10μg/kg/day for 4 days).

    Recombinant:

    Article Title: Cytokine therapy of acute radiation syndrome.
    Article Snippet: Radiological accidents/incidents are common with nearly 400 reported since 1944 exposing about 3000 people to substantial doses of ionizing radiations with 127 deaths.. Damage to hematopoietic stem and progenitor cells with resulting bone marrow failure is a common consequence of exposure to whole body acute high-dose and -dose-rate ionizing radiations and is termed hematopoietic-acute radiation syndrome, or H-ARS.. Therapy of H-ARS includes transfusions, anti-bacterial and -viral drugs, molecularly-cloned hematopoietic growth factors and hematopoietic cell transplants.

    Article Title: Sargramostim in acute radiation syndrome.
    Article Snippet: Three molecularly cloned hematopoietic growth factors are US FDA-approved to increase survival in adult and pediatric patients acutely exposed to myelosuppressive doses of radiation (hematopoietic syndrome of acute radiation syndrome, or ‘H-ARS’ [21–23]) and accelerate bone marrow recovery after exposure to acute, high-dose, and high-dose-rate ionizing radiation in accidents and other settings. .. These include recombinant human granulocyte colony-stimulating factor (rhu G-CSF, filgrastim, NeupogenTM; Amgen, Thousand Oaks, CA); pegylated rh G-CSF (pegylated filgrastim, NeulastaTM, Amgen); and recombinant human granulocyte-macrophage colonystimulating factor (rhu GM-CSF, sargramostim, Leukine, Partner Therapeutics, Lexington, MA) [21–25]. ..



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    Image Search Results


    BDNF lower in AIS/DM patients. (a) Expression of plasma BDNF in healthy controls, AIS/non-DM patients, and AIS/DM patients. (b) Representative images of immunohistochemical detection of BDNF (brown) in clots from AIS/non-DM and AIS/DM patients. (c) The column graph represents mean ± SEM of staining scores in each group. (d) Representative clots from AIS/non-DM and AIS/DM patients were stained using MSB to visualize the fibrin (dark pink to red), RBCs (yellow), and platelets (gray). (e) Column graph displays the differences in fibrin content in clots. (f) Fibrin fluorescence of clots from AIS patients. (g) The bar graph represents mean ± SEM fluorescence values.

    Journal: Journal of Diabetes Research

    Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

    doi: 10.1155/jdr/6693363

    Figure Lengend Snippet: BDNF lower in AIS/DM patients. (a) Expression of plasma BDNF in healthy controls, AIS/non-DM patients, and AIS/DM patients. (b) Representative images of immunohistochemical detection of BDNF (brown) in clots from AIS/non-DM and AIS/DM patients. (c) The column graph represents mean ± SEM of staining scores in each group. (d) Representative clots from AIS/non-DM and AIS/DM patients were stained using MSB to visualize the fibrin (dark pink to red), RBCs (yellow), and platelets (gray). (e) Column graph displays the differences in fibrin content in clots. (f) Fibrin fluorescence of clots from AIS patients. (g) The bar graph represents mean ± SEM fluorescence values.

    Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

    Techniques: Expressing, Clinical Proteomics, Immunohistochemical staining, Staining, Fluorescence

    The clot permeability and ultrastructure. (a) Thrombus permeability was significantly lower in AIS/DM patients than AIS patients without DM. (b) Correlation between BDNF concentrations and thrombus permeability. (c) BDNF levels in patients based on the severity of stroke. (d) Scanning electron micrographs of thrombi. Arrows indicate fibrin; dotted lines indicate fibrin band. Bar = 20 μ m. (e) Transmission electron micrographs of thrombi from the AIS/non-DM and AIS/DM patients. Arrows indicate fibrin; dotted lines indicate fibrin band. Bar = 5 μ m.

    Journal: Journal of Diabetes Research

    Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

    doi: 10.1155/jdr/6693363

    Figure Lengend Snippet: The clot permeability and ultrastructure. (a) Thrombus permeability was significantly lower in AIS/DM patients than AIS patients without DM. (b) Correlation between BDNF concentrations and thrombus permeability. (c) BDNF levels in patients based on the severity of stroke. (d) Scanning electron micrographs of thrombi. Arrows indicate fibrin; dotted lines indicate fibrin band. Bar = 20 μ m. (e) Transmission electron micrographs of thrombi from the AIS/non-DM and AIS/DM patients. Arrows indicate fibrin; dotted lines indicate fibrin band. Bar = 5 μ m.

    Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

    Techniques: Permeability, Transmission Assay

    BDNF influences fibrin density. (a) A ribbon diagram illustrates the binding of BDNF to the heparin-binding domain of fibrin(ogen), with the structure generated via DMFold. (b) rHu-BDNF was added to plasma pools from AIS/DM patients before induction of coagulation with thrombin; then, the fibrin density of the clot was analyzed. Visualization images of clots using rabbit polyclonal fibrinogen/FITC (bar = 100 μ m) and (c) the percentage of fibrin fibers versus control. Fibrin fibers were analyzed using ImageJ software. All samples were performed in triplicate. Data are expressed as mean ± SEM, ⁣ ∗ p < 0.05.

    Journal: Journal of Diabetes Research

    Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

    doi: 10.1155/jdr/6693363

    Figure Lengend Snippet: BDNF influences fibrin density. (a) A ribbon diagram illustrates the binding of BDNF to the heparin-binding domain of fibrin(ogen), with the structure generated via DMFold. (b) rHu-BDNF was added to plasma pools from AIS/DM patients before induction of coagulation with thrombin; then, the fibrin density of the clot was analyzed. Visualization images of clots using rabbit polyclonal fibrinogen/FITC (bar = 100 μ m) and (c) the percentage of fibrin fibers versus control. Fibrin fibers were analyzed using ImageJ software. All samples were performed in triplicate. Data are expressed as mean ± SEM, ⁣ ∗ p < 0.05.

    Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

    Techniques: Binding Assay, Generated, Clinical Proteomics, Coagulation, Control, Software

    Observing the fibrin diameter by scanning electron microscopy. (a) Fibrin structures formed from venous blood from AIS/DM subjects were added with different concentrations of BDNF. The ultrastructure of fibrin was observed by a scanning electron microscope. (b) The column chart shows the difference in the diameter of fibrin fibers in clots of different groups. ⁣ ∗ p < 0.05 and ⁣ ∗∗∗ p < 0.001.

    Journal: Journal of Diabetes Research

    Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

    doi: 10.1155/jdr/6693363

    Figure Lengend Snippet: Observing the fibrin diameter by scanning electron microscopy. (a) Fibrin structures formed from venous blood from AIS/DM subjects were added with different concentrations of BDNF. The ultrastructure of fibrin was observed by a scanning electron microscope. (b) The column chart shows the difference in the diameter of fibrin fibers in clots of different groups. ⁣ ∗ p < 0.05 and ⁣ ∗∗∗ p < 0.001.

    Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

    Techniques: Electron Microscopy, Microscopy

    rHu-BDNF influences fibrin polymerization, coagulation, and lysis. (a) Representative turbidity curves were recorded spectrophotometrically at 350 nm (37°C) with 30-s intervals. (b) Maximum turbidity values were determined under identical monitoring conditions. (c) Maximum clot firmness was assessed by thromboelastographic analyses (MA). Plasma samples supplemented with recombinant BDNF prior to thrombin/tPA-induced coagulation underwent subsequent polymerization analysis. (d) Thrombin clotting time (TT) and (e) lysis time were measured. All samples were performed in triplicate. Data are expressed as mean ± SEM, n = 3 different pools. ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, and ⁣ ∗∗∗ p < 0.001.

    Journal: Journal of Diabetes Research

    Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

    doi: 10.1155/jdr/6693363

    Figure Lengend Snippet: rHu-BDNF influences fibrin polymerization, coagulation, and lysis. (a) Representative turbidity curves were recorded spectrophotometrically at 350 nm (37°C) with 30-s intervals. (b) Maximum turbidity values were determined under identical monitoring conditions. (c) Maximum clot firmness was assessed by thromboelastographic analyses (MA). Plasma samples supplemented with recombinant BDNF prior to thrombin/tPA-induced coagulation underwent subsequent polymerization analysis. (d) Thrombin clotting time (TT) and (e) lysis time were measured. All samples were performed in triplicate. Data are expressed as mean ± SEM, n = 3 different pools. ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, and ⁣ ∗∗∗ p < 0.001.

    Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

    Techniques: Coagulation, Lysis, Clinical Proteomics, Recombinant

    Taxa used for molecular phylogenetic analyses and their GenBank accession numbers. (T) = ex-type strains. Isolates in this study are shown in bold.

    Journal: MycoKeys

    Article Title: New species and records of Phaeobotryon ( Botryosphaeriales , Botryosphaeriaceae ) from Larix in China

    doi: 10.3897/mycokeys.112.139053

    Figure Lengend Snippet: Taxa used for molecular phylogenetic analyses and their GenBank accession numbers. (T) = ex-type strains. Isolates in this study are shown in bold.

    Article Snippet: P. rhois , CFCC 89662 = CCTCC AF2014017 T , Rhus typhina , China , Km030584 , Km030591 , Km030598.

    Techniques:

    Classification of honey types and regional sources.

    Journal: Food Science & Nutrition

    Article Title: Protective Effects of Some Graded Iranian Honey Samples Against Cold Water Immersion‐Induced Gastric Ulcers in Rats

    doi: 10.1002/fsn3.4567

    Figure Lengend Snippet: Classification of honey types and regional sources.

    Article Snippet: H5 , Sumaq , Rhus coriaria , Anacardiaceae , Gonabad/Razavi Khorasan province.

    Techniques: