Journal: Journal of Diabetes Research

Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

doi: 10.1155/jdr/6693363

Figure Lengend Snippet: BDNF lower in AIS/DM patients. (a) Expression of plasma BDNF in healthy controls, AIS/non-DM patients, and AIS/DM patients. (b) Representative images of immunohistochemical detection of BDNF (brown) in clots from AIS/non-DM and AIS/DM patients. (c) The column graph represents mean ± SEM of staining scores in each group. (d) Representative clots from AIS/non-DM and AIS/DM patients were stained using MSB to visualize the fibrin (dark pink to red), RBCs (yellow), and platelets (gray). (e) Column graph displays the differences in fibrin content in clots. (f) Fibrin fluorescence of clots from AIS patients. (g) The bar graph represents mean ± SEM fluorescence values.

Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

Techniques: Expressing, Clinical Proteomics, Immunohistochemical staining, Staining, Fluorescence

Journal: Journal of Diabetes Research

Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

doi: 10.1155/jdr/6693363

Figure Lengend Snippet: The clot permeability and ultrastructure. (a) Thrombus permeability was significantly lower in AIS/DM patients than AIS patients without DM. (b) Correlation between BDNF concentrations and thrombus permeability. (c) BDNF levels in patients based on the severity of stroke. (d) Scanning electron micrographs of thrombi. Arrows indicate fibrin; dotted lines indicate fibrin band. Bar = 20 μ m. (e) Transmission electron micrographs of thrombi from the AIS/non-DM and AIS/DM patients. Arrows indicate fibrin; dotted lines indicate fibrin band. Bar = 5 μ m.

Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

Techniques: Permeability, Transmission Assay

Journal: Journal of Diabetes Research

Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

doi: 10.1155/jdr/6693363

Figure Lengend Snippet: BDNF influences fibrin density. (a) A ribbon diagram illustrates the binding of BDNF to the heparin-binding domain of fibrin(ogen), with the structure generated via DMFold. (b) rHu-BDNF was added to plasma pools from AIS/DM patients before induction of coagulation with thrombin; then, the fibrin density of the clot was analyzed. Visualization images of clots using rabbit polyclonal fibrinogen/FITC (bar = 100 μ m) and (c) the percentage of fibrin fibers versus control. Fibrin fibers were analyzed using ImageJ software. All samples were performed in triplicate. Data are expressed as mean ± SEM, ⁣ ∗ p < 0.05.

Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

Techniques: Binding Assay, Generated, Clinical Proteomics, Coagulation, Control, Software

Journal: Journal of Diabetes Research

Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

doi: 10.1155/jdr/6693363

Figure Lengend Snippet: Observing the fibrin diameter by scanning electron microscopy. (a) Fibrin structures formed from venous blood from AIS/DM subjects were added with different concentrations of BDNF. The ultrastructure of fibrin was observed by a scanning electron microscope. (b) The column chart shows the difference in the diameter of fibrin fibers in clots of different groups. ⁣ ∗ p < 0.05 and ⁣ ∗∗∗ p < 0.001.

Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

Techniques: Electron Microscopy, Microscopy

Journal: Journal of Diabetes Research

Article Title: Plasma BDNF Is Reduced in Acute Ischemic Stroke Patients With Type 2 Diabetes Mellitus and Associated With Fibrin-Rich Thrombi

doi: 10.1155/jdr/6693363

Figure Lengend Snippet: rHu-BDNF influences fibrin polymerization, coagulation, and lysis. (a) Representative turbidity curves were recorded spectrophotometrically at 350 nm (37°C) with 30-s intervals. (b) Maximum turbidity values were determined under identical monitoring conditions. (c) Maximum clot firmness was assessed by thromboelastographic analyses (MA). Plasma samples supplemented with recombinant BDNF prior to thrombin/tPA-induced coagulation underwent subsequent polymerization analysis. (d) Thrombin clotting time (TT) and (e) lysis time were measured. All samples were performed in triplicate. Data are expressed as mean ± SEM, n = 3 different pools. ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, and ⁣ ∗∗∗ p < 0.001.

Article Snippet: Citrated plasma aliquots (100 μ L), underwent 15-min preincubation at 37°C with either (a) 1- μ L distilled water containing graded recombinant human BDNF (rHu-BDNF) qualities (0.1, 0.2, or 0.3 ng, Solarbio P00062) or (b) equivalent-volume distilled water (control).

Techniques: Coagulation, Lysis, Clinical Proteomics, Recombinant

Journal: MycoKeys

Article Title: New species and records of Phaeobotryon ( Botryosphaeriales , Botryosphaeriaceae ) from Larix in China

doi: 10.3897/mycokeys.112.139053

Figure Lengend Snippet: Taxa used for molecular phylogenetic analyses and their GenBank accession numbers. (T) = ex-type strains. Isolates in this study are shown in bold.

Article Snippet: P. rhois , CFCC 89662 = CCTCC AF2014017 T , Rhus typhina , China , Km030584 , Km030591 , Km030598.

Techniques:

Journal: Food Science & Nutrition

Article Title: Protective Effects of Some Graded Iranian Honey Samples Against Cold Water Immersion‐Induced Gastric Ulcers in Rats

doi: 10.1002/fsn3.4567

Figure Lengend Snippet: Classification of honey types and regional sources.

Article Snippet: H5 , Sumaq , Rhus coriaria , Anacardiaceae , Gonabad/Razavi Khorasan province.

Techniques: