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anti senescence marker protein 30  (Proteintech)


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    Proteintech anti senescence marker protein 30
    Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence <t>marker</t> <t>protein‐30</t> <t>(SMP30),</t> Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Anti Senescence Marker Protein 30, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 19 article reviews
    anti senescence marker protein 30 - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "NMNAT1 Activates Autophagy to Delay D‐Galactose‐Induced Aging in Cochlear Hair Cells"

    Article Title: NMNAT1 Activates Autophagy to Delay D‐Galactose‐Induced Aging in Cochlear Hair Cells

    Journal: Aging Cell

    doi: 10.1111/acel.70373

    Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: CCK-8 Assay, Staining, Flow Cytometry, Western Blot, Marker, Expressing

    Establishment of D‐gal‐induced aging model in cochlear explants. (A) Immunofluorescence staining demonstrating the number of myosin7a + hair cells in cochlear explants treated with 20, 30, and 40 mg/mL D‐gal for 72 h. (B) Quantification of the number of myosin7a + hair cells in cochlear explants ( n = 4; four individual explants from four mice). (C–E) Western blotting of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X expression in cochlear explants exposed to D‐gal. (F–H) Statistical analysis of SMP30 ( n = 5), Lamin B1 ( n = 3), and γ‐H2A.X ( n = 3) in C–E; n corresponds to the number of independent samples, each consisting of 12 explants from six mice. (I) Senescence‐associated β‐gal staining images of hair cells in cochlear explants exposed to D‐gal. (J) Quantification of β‐gal staining intensity in I ( n = 4; four individual explants from four mice). Scale bar: 20 μm. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: Establishment of D‐gal‐induced aging model in cochlear explants. (A) Immunofluorescence staining demonstrating the number of myosin7a + hair cells in cochlear explants treated with 20, 30, and 40 mg/mL D‐gal for 72 h. (B) Quantification of the number of myosin7a + hair cells in cochlear explants ( n = 4; four individual explants from four mice). (C–E) Western blotting of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X expression in cochlear explants exposed to D‐gal. (F–H) Statistical analysis of SMP30 ( n = 5), Lamin B1 ( n = 3), and γ‐H2A.X ( n = 3) in C–E; n corresponds to the number of independent samples, each consisting of 12 explants from six mice. (I) Senescence‐associated β‐gal staining images of hair cells in cochlear explants exposed to D‐gal. (J) Quantification of β‐gal staining intensity in I ( n = 4; four individual explants from four mice). Scale bar: 20 μm. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: Immunofluorescence, Staining, Western Blot, Marker, Expressing



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    Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence <t>marker</t> <t>protein‐30</t> <t>(SMP30),</t> Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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    Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence <t>marker</t> <t>protein‐30</t> <t>(SMP30),</t> Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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    Image Search Results


    Taq Man Probes used in the validation of the NGS results experiment.

    Journal: Scientific Reports

    Article Title: Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

    doi: 10.1038/s41598-025-98918-9

    Figure Lengend Snippet: Taq Man Probes used in the validation of the NGS results experiment.

    Article Snippet: RGN , Ss03380996_u1.

    Techniques: Biomarker Discovery

    Primers used for quantitative methylation-specific PCR.

    Journal: Scientific Reports

    Article Title: Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

    doi: 10.1038/s41598-025-98918-9

    Figure Lengend Snippet: Primers used for quantitative methylation-specific PCR.

    Article Snippet: RGN , Ss03380996_u1.

    Techniques: Methylation, Amplification

    Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Aging Cell

    Article Title: NMNAT1 Activates Autophagy to Delay D‐Galactose‐Induced Aging in Cochlear Hair Cells

    doi: 10.1111/acel.70373

    Figure Lengend Snippet: Establishment of D‐gal‐treated aging model in HEI‐OC1 cells. (A) Schematic illustration of D‐gal treatment in HEI‐OC1 cells. (B) Cell viability after exposure to different concentrations of D‐gal, evaluated using the CCK‐8 assay ( n = 4, independent samples). (C) Immunofluorescent staining of Mito‐SOX in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (D) Quantitative analysis of Mito‐SOX intensity in C ( n = 4, independent samples). (E) Mito‐SOX levels were detected using flow cytometry in HEI‐OC1 cells exposed to D‐gal (5 and 20 mg/mL) for 72 h. (F) Statistical analysis of Mito‐SOX levels in E ( n = 3, independent samples). (G) Immunofluorescent staining of DCFH‐DA in HEI‐OC1 cells. (H) Quantitative analysis of DCFH‐DA intensity in G ( n = 4, independent samples). (I) DCFH‐DA levels were detected using flow cytometry in HEI‐OC1 cells. (J) Statistical analysis of DCFH‐DA levels in I ( n = 3, independent samples). (K–M) Western blot analysis of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X in HEI‐OC1 cells treated with D‐gal. (N–P) Statistical analysis of SMP30 ( n = 4, independent samples), Lamin B1 ( n = 3, independent samples), and γ‐H2A.X ( n = 3, independent samples) expression in K–M. Scale bar: 20 μm. Statistical significance as ns, no significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies used in this study were as follows: rabbit anti‐β‐tubulin (1:40000, 5568; CST), rabbit anti‐β‐Actin (1:40000, 20536‐1‐AP; Proteintech), mouse anti‐GAPDH (1:4000, ab8245; Abcam), rabbit anti‐senescence marker protein‐30 (SMP30) (1:1000, 17947‐1‐AP; Proteintech), rabbit anti‐lamin B1 (1:1000, 13435; CST), rabbit anti‐γ‐H2A.X (1:400, 2577; CST), rabbit anti‐P21 (1:1000, 37543; CST), rabbit anti‐P16 (1:1000, 29271; CST), rabbit anti‐NMNAT1 (1:1000, 98354; CST), rabbit anti‐NMNAT1 (1:400, 11399‐1‐AP; Proteintech), rabbit anti‐LC3B (1:1000, 3868; CST), rabbit anti‐P62 (1:1000, 23214; CST), mouse anti‐autophagy related 7 (ATG7) (1:1000, 67341; Proteintech), and rabbit anti‐BECLIN1 (1:1000, 3495; CST).

    Techniques: CCK-8 Assay, Staining, Flow Cytometry, Western Blot, Marker, Expressing

    Establishment of D‐gal‐induced aging model in cochlear explants. (A) Immunofluorescence staining demonstrating the number of myosin7a + hair cells in cochlear explants treated with 20, 30, and 40 mg/mL D‐gal for 72 h. (B) Quantification of the number of myosin7a + hair cells in cochlear explants ( n = 4; four individual explants from four mice). (C–E) Western blotting of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X expression in cochlear explants exposed to D‐gal. (F–H) Statistical analysis of SMP30 ( n = 5), Lamin B1 ( n = 3), and γ‐H2A.X ( n = 3) in C–E; n corresponds to the number of independent samples, each consisting of 12 explants from six mice. (I) Senescence‐associated β‐gal staining images of hair cells in cochlear explants exposed to D‐gal. (J) Quantification of β‐gal staining intensity in I ( n = 4; four individual explants from four mice). Scale bar: 20 μm. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Aging Cell

    Article Title: NMNAT1 Activates Autophagy to Delay D‐Galactose‐Induced Aging in Cochlear Hair Cells

    doi: 10.1111/acel.70373

    Figure Lengend Snippet: Establishment of D‐gal‐induced aging model in cochlear explants. (A) Immunofluorescence staining demonstrating the number of myosin7a + hair cells in cochlear explants treated with 20, 30, and 40 mg/mL D‐gal for 72 h. (B) Quantification of the number of myosin7a + hair cells in cochlear explants ( n = 4; four individual explants from four mice). (C–E) Western blotting of senescence marker protein‐30 (SMP30), Lamin B1, and γ‐H2A.X expression in cochlear explants exposed to D‐gal. (F–H) Statistical analysis of SMP30 ( n = 5), Lamin B1 ( n = 3), and γ‐H2A.X ( n = 3) in C–E; n corresponds to the number of independent samples, each consisting of 12 explants from six mice. (I) Senescence‐associated β‐gal staining images of hair cells in cochlear explants exposed to D‐gal. (J) Quantification of β‐gal staining intensity in I ( n = 4; four individual explants from four mice). Scale bar: 20 μm. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies used in this study were as follows: rabbit anti‐β‐tubulin (1:40000, 5568; CST), rabbit anti‐β‐Actin (1:40000, 20536‐1‐AP; Proteintech), mouse anti‐GAPDH (1:4000, ab8245; Abcam), rabbit anti‐senescence marker protein‐30 (SMP30) (1:1000, 17947‐1‐AP; Proteintech), rabbit anti‐lamin B1 (1:1000, 13435; CST), rabbit anti‐γ‐H2A.X (1:400, 2577; CST), rabbit anti‐P21 (1:1000, 37543; CST), rabbit anti‐P16 (1:1000, 29271; CST), rabbit anti‐NMNAT1 (1:1000, 98354; CST), rabbit anti‐NMNAT1 (1:400, 11399‐1‐AP; Proteintech), rabbit anti‐LC3B (1:1000, 3868; CST), rabbit anti‐P62 (1:1000, 23214; CST), mouse anti‐autophagy related 7 (ATG7) (1:1000, 67341; Proteintech), and rabbit anti‐BECLIN1 (1:1000, 3495; CST).

    Techniques: Immunofluorescence, Staining, Western Blot, Marker, Expressing