Journal: Journal of Biological Chemistry

Article Title: β-Catenin Regulates Vitamin C Biosynthesis and Cell Survival in Murine Liver

doi: 10.1074/jbc.m109.047258

Figure Lengend Snippet: FIGURE 3. Regucalcin, which is expressed in both Hep3B cells and HepG2 cells, also associates with -catenininthecell.A,WesternblotofproteinsharvestedfromHepG2andHep3Bhumanhepatomacelllines showsthatHepG2cellsexpresshigherlevelsofregucalcinthanHep3Bcells.Westernblotforactinservesasthe loading control. B, real time PCR analysis of mRNA harvested from HepG2 and Hep3B human hepatoma cell lines. Regucalcin mRNA expression was 6-fold higher in Hep3B cells but more than 200-fold higher in HepG2 cells as compared with average of normal human livers. Regucalcin mRNA expression was standardized to actin expression. C, immunofluorescence on cultured HepG2 and Hep3B cells demonstrates higher regucalcin (green) and -catenin (red) in HepG2 cells than Hep3B cells. Also, as seen in bottom panels, colocalization (white arrows) of -catenin and regucalcin was evident especially in HepG2 cells. Images were taken at 600 magni- fication. D, HepG2 and Hep3B cell lysates immunoprecipitated with -catenin antibody and probed for regu- calcin (top) show association of the two proteins. Nonspecific (N) IgG did not pull down regucalcin. Similar lysates immunoprecipitated with regucalcin (SMP30) antibody (Ab) and probed for -catenin also show asso- ciation of full-length -catenin with regucalcin in both cell types and truncated -catenin-regucalcin associa- tioninHepG2cells.Successfulpulldownof-cateninandregucalcinbytheirantibodiesisverifiedinrespective lower panels.

Article Snippet: Primary antibodies against -catenin (1:100) and SMP30 (also known as regucalcin) (1:100) (Santa Cruz Biotechnology) were utilized.

Techniques: Control, Real-time Polymerase Chain Reaction, Expressing, Immunofluorescence, Cell Culture, Immunoprecipitation

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Lentiviral vectors: LV5: GP96 lentiviral vector structure; LV8: SMP30 lentiviral vector structure. (a) GP96-transfected DCs. (b) SMP30-transfected DCs. (a) and (b) show the same location.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Plasmid Preparation, Transfection

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: WB after transfection. (a) Detection of GP96 in 6 groups. (b) Detection of SMP30 in 6 groups. (c) GAPDH as the internal control. The GP96 group expressed more GP96, and the SMP30 group expressed more SMP30, indicating that transfection was successful.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Transfection, Control

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Liver cancer model. (a) The liver cancer model. (b) The GP96 + SMP30 group: tumors had disappeared in some mice on day 4. (c) Tumors harvested from mice. (d) Negative TUNEL staining for apoptosis in the DC group. (e) Positive TUNEL staining for apoptosis in the GP96 + SMP30 group.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: TUNEL Assay, Staining

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Tumor growth curve and ELISA results. Curve: the tumor volume in the GP96 + SMP30 group was smaller than that in the protein group on day 4, day 14, and day 16, P < 0.05. IL-2: the GP96 + SMP30 group showed secretion of more IL-2 than the other groups, P < 0.01. IFN- γ : the GP96 + SMP30 group showed secretion of more IFN- γ than the other groups, P < 0.01.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Immunohistochemical staining of tumors. (a) Tumor expressing IL-2 in the GP96 + SMP30 group; the brown cytoplasm indicates positive staining. (b) Tumor expressing IL-2 in the GP96 group; the colorless cytoplasm indicates negative staining. (c) Tumor expressing IFN- γ in the GP96 + SMP30 group; the brown cytoplasm indicates positive staining. (d) Tumor expressing IFN- γ in the SMP30 group; the colorless cytoplasm indicates negative staining.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Immunohistochemical staining, Staining, Expressing, Negative Staining

Journal: Scientific Reports

Article Title: Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

doi: 10.1038/s41598-025-98918-9

Figure Lengend Snippet: Taq Man Probes used in the validation of the NGS results experiment.

Article Snippet: RGN , Ss03380996_u1.

Techniques: Biomarker Discovery

Journal: Scientific Reports

Article Title: Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

doi: 10.1038/s41598-025-98918-9

Figure Lengend Snippet: Primers used for quantitative methylation-specific PCR.

Article Snippet: RGN , Ss03380996_u1.

Techniques: Methylation, Amplification

Journal: PLoS ONE

Article Title: Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

doi: 10.1371/journal.pone.0016545

Figure Lengend Snippet: HEK-293A cells (A) and C3A liver cells (B) were treated with the indicated number of viral particles for 72 h and the expression of SMP30-HA was analyzed by western blotting using SMP30 or HA antibodies.

Article Snippet: SMP30 antibody was supplied by Cosmo Bio Co. (Denver, CO) and HA-probe antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Expressing, Western Blot

Journal: PLoS ONE

Article Title: Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

doi: 10.1371/journal.pone.0016545

Figure Lengend Snippet: Rat liver cytosolic extract in increasing amounts (10, 20 and 30 µg total protein marked as 1, 2 and 3) were subjected to western blotting using antibody against SMP30. Extracts of C3A liver cells expressed SMP30-HA was shown for comparison.

Article Snippet: SMP30 antibody was supplied by Cosmo Bio Co. (Denver, CO) and HA-probe antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

doi: 10.1371/journal.pone.0016545

Figure Lengend Snippet: The animals were injected with the control virus (Ad-null, A & B) or Ad-SMP30-HA (C & D) via the tail vein at a dose of 2×10 11 VP/animal and sacrificed on day 4 post-virus injection. Tissue homogenates were processed by western blotting using anti-HA antibody.

Article Snippet: SMP30 antibody was supplied by Cosmo Bio Co. (Denver, CO) and HA-probe antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Injection, Western Blot

Journal: PLoS ONE

Article Title: Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

doi: 10.1371/journal.pone.0016545

Figure Lengend Snippet: HEK-293A cell (A) and C3A cells were treated with Ad-SMP30-HA (20 VP/cell for HEK-293A cells and 500 VP/cell for C3A liver cells) for varying periods of time, cell extracts were prepared in M-PER buffer and separated into the particulate and cytosolic fractions. Western blotting was carried out using antibody against HA tag.

Article Snippet: SMP30 antibody was supplied by Cosmo Bio Co. (Denver, CO) and HA-probe antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

doi: 10.1371/journal.pone.0016545

Figure Lengend Snippet: A, The inhibitors, MGI32 (10 µM), PSI (50 µM), LCys (10 µM), chloroquine (50 µM) and tunicamycin (3 µg/ml) were added along with Ad-SMP30-HA (20 VP/cell) to the cells and incubated for 24 h; B, Effect of proteasomal degradation system on the processing of SMP30 protein expressed in HEK-293A cells in the presence or absence of 10 µM MG132; C, Varying concentrations of calpain inhibitor I (CI, 5, 10, 15 and 20 µM) or MDL28170 (MDL, 5, 10 and 15 µM) were added to the cells along with Ad-SMP30-HA (20 VP/cell) and incubated for 24 h; D, HEK-293A cells were treated with 20 µM caspase inhibitor I [Z-VAD(OMe)-FMK] or 5 µM β-secretase inhibitor (Z-VLL-CHO) or 5 µM γ-secretase inhibitor (Z-LLNle-CHO) or 10 µM MG132 along with Ad-SMP30-HA (20 VP/cell) for 24 h; Equal volume of DMSO served as control. The cells were harvested, lysed with SDS-PAGE buffer containing 5% β-mercaptoethanol and SDS-PAGE followed by western blotting was carried out using anti-HA antibody.

Article Snippet: SMP30 antibody was supplied by Cosmo Bio Co. (Denver, CO) and HA-probe antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Incubation, SDS Page, Western Blot

Journal: Food Science & Nutrition

Article Title: Optimization of Ultrasound Extraction of Total Anthocyanin From Berberis kaschgarica Rupr. by Response Surface Methodology and Its Antihypertensive Effect

doi: 10.1002/fsn3.4591

Figure Lengend Snippet: Serum level of (A) renin (REN); (B) angiotensin‐converting enzyme (ACE); (C) angiotensin II (Ang‐II); (D) angiotensin‐(1–7) (Ang‐(1–7)); (E) Endothelin‐1 (ET‐1); and (F) nitric oxide (NO) after 12 weeks of treatment of Wistar–Kyoto (WKY) rats and spontaneously hypertensive (SHR) rats groups. WKY group (distilled water), SHR group (distilled water), SHR + CAP group (12.5 mg/kg of captopril), SHR + BKRA‐L (50 mg/kg body of purified extract of B. kaschgarica anthocyanins), SHR + BKRA‐M (100 mg/kg of purified extract of B. kaschgarica anthocyanins), and SHR + BKRA‐H (200 mg/kg of purified extract of B. kaschgarica anthocyanins) were administered for 12 weeks by oral gavage. Each group contains 10 rats. The data are presented as mean ± standard deviation. One‐way analysis of variance (ANOVA) followed by Tukey's post hoc test, significance difference: ### p < 0.001 versus WKY group and * p < 0.05, ** p < 0.01, *** p < 0.001 versus SHR group.

Article Snippet: The REN, ACE, Ang‐II, and Ang‐(1–7) Elisa kits were purchased from Elebscience (Elabscience, Wuhan, China), while the ET‐1 and NO Elisa kits were obtained from Shanghai Elisa Biotech (Shanghai Elisa Biotech Co. Ltd. China) and Beyotime Biotechnology (Beyotime Biotechnology, Shanghai, China), respectively.

Techniques: Purification, Standard Deviation