psti (TaKaRa)
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psti
Psti, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psti/Pst+I/pm42816489-401-5-8
Average 96 stars, based on 1870 article reviews
Psti, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psti/Pst+I/pm42816489-401-5-8
Average 96 stars, based on 1870 article reviews
psti - by Bioz Stars,
2026-10
96/100 stars
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Polymerase Chain Reaction:Article Title: Mannose metabolic pathway senses glucose supply and regulates cell fate decisions. Article Snippet: Polymerase chain reaction and restriction enzyme digestion 5 PCR was performed using cDNA (500 ng), Phusion High-Fidelity DNA Polymerase (M0530, New England Biolabs, Ipswich, MA, USA), and primers (Tabel 1) in a 20 μL reaction mixture according to the manufacturer’s instructions. .. To analyze XBP1 mRNA splicing, the PCR products were incubated with or without Incubation:Article Title: Mannose metabolic pathway senses glucose supply and regulates cell fate decisions. Article Snippet: Polymerase chain reaction and restriction enzyme digestion 5 PCR was performed using cDNA (500 ng), Phusion High-Fidelity DNA Polymerase (M0530, New England Biolabs, Ipswich, MA, USA), and primers (Tabel 1) in a 20 μL reaction mixture according to the manufacturer’s instructions. .. To analyze XBP1 mRNA splicing, the PCR products were incubated with or without Article Title: Submolecular video-imaging of the Smc5/6 complex topologically bound to DNA. Article Snippet: .. Thewashed beads were incubated with Amplification:Article Title: Lipid production control factor for oleaginous yeast Article Snippet: A PCR reaction solution (composition: KOD One Master Mix (Toyobo): 25 μL, 10 μM 115694 kpn Fw (5′-ggggtaccATGGACCCGTTAATGGACAATG-3′ (SEQ ID NO: 3)): 1.5 μL, 10 μM 115694 pst Rv (5′-ttctgcagTCACTCAACTAGTACAATCCGATTCTG-3′ (SEQ ID NO: 4)): 1.5 μL, template (reverse transcription solution of L. starkeyi CBS1807): 10 μL, sterile water: 12 μL) was prepared, and 115694 cDNA was amplified under the following PCR reaction conditions (first denaturation at 98° C. for 2 minutes; then 10 seconds at 98° C., 5 seconds at 58° C., and 40 seconds at 68° C. for 30 cycles; and final elongation at 68° C. for 1 minute). .. The amplified fragment was purified by phenol-chloroform/ethanol precipitation, and after digestion with KpnI (TaKaRa) and Article Title: Contribution of a Novel TetR/AcrR Family Transcriptional Regulator RalT of Ralstonia pseudosolanacearum Strain OE1‐1 to the Fine‐Tuning of Its Virulence Article Snippet: .. The amplified fragments were digested with Article Title: Contribution of a Novel TetR/AcrR Family Transcriptional Regulator RalT of Ralstonia pseudosolanacearum Strain OE1-1 to the Fine-Tuning of Its Virulence. Article Snippet: .. The amplified fragments were digested with Purification:Article Title: Lipid production control factor for oleaginous yeast Article Snippet: A PCR reaction solution (composition: KOD One Master Mix (Toyobo): 25 μL, 10 μM 115694 kpn Fw (5′-ggggtaccATGGACCCGTTAATGGACAATG-3′ (SEQ ID NO: 3)): 1.5 μL, 10 μM 115694 pst Rv (5′-ttctgcagTCACTCAACTAGTACAATCCGATTCTG-3′ (SEQ ID NO: 4)): 1.5 μL, template (reverse transcription solution of L. starkeyi CBS1807): 10 μL, sterile water: 12 μL) was prepared, and 115694 cDNA was amplified under the following PCR reaction conditions (first denaturation at 98° C. for 2 minutes; then 10 seconds at 98° C., 5 seconds at 58° C., and 40 seconds at 68° C. for 30 cycles; and final elongation at 68° C. for 1 minute). .. The amplified fragment was purified by phenol-chloroform/ethanol precipitation, and after digestion with KpnI (TaKaRa) and Ligation:Article Title: Lipid production control factor for oleaginous yeast Article Snippet: A PCR reaction solution (composition: KOD One Master Mix (Toyobo): 25 μL, 10 μM 115694 kpn Fw (5′-ggggtaccATGGACCCGTTAATGGACAATG-3′ (SEQ ID NO: 3)): 1.5 μL, 10 μM 115694 pst Rv (5′-ttctgcagTCACTCAACTAGTACAATCCGATTCTG-3′ (SEQ ID NO: 4)): 1.5 μL, template (reverse transcription solution of L. starkeyi CBS1807): 10 μL, sterile water: 12 μL) was prepared, and 115694 cDNA was amplified under the following PCR reaction conditions (first denaturation at 98° C. for 2 minutes; then 10 seconds at 98° C., 5 seconds at 58° C., and 40 seconds at 68° C. for 30 cycles; and final elongation at 68° C. for 1 minute). .. The amplified fragment was purified by phenol-chloroform/ethanol precipitation, and after digestion with KpnI (TaKaRa) and other:Article Title: Mannose metabolic pathway senses glucose supply and regulates cell fate decisions Article Snippet: The solvent system was as follows: solvent A, 100% acetonitrile (018-19853, FUJIFILM Wako Pure Chemical Corporation); solvent B, 0.4% formic acid (067-04531, FUJIFILM Wako Pure Chemical Corporation) in water adjusted to pH 4.4 with ammonia (02512-95, Nacalai Tesque). Derivative Assay:Article Title: Contribution of a Novel TetR/AcrR Family Transcriptional Regulator RalT of Ralstonia pseudosolanacearum Strain OE1‐1 to the Fine‐Tuning of Its Virulence Article Snippet: .. The amplified fragments were digested with Article Title: Contribution of a Novel TetR/AcrR Family Transcriptional Regulator RalT of Ralstonia pseudosolanacearum Strain OE1-1 to the Fine-Tuning of Its Virulence. Article Snippet: .. The amplified fragments were digested with |
![Stress‐induced BEVs are internalised by recipient cells and mediate horizontal gene transfer (HGT). (a) HGT rate of the tetR ‐cassette using BEVs derived from VC1620/1:: tetR (BEVs VC1620/1:: tetR ) as donor. BEVs VC1620/1:: tetR were isolated from AKI cultures in presence of bile (17.25 mM) or MMC (60 ng mL −1 ) or without any stressor (control, co). In addition, trypsin digested BEVs VC1620/1:: tetR derived from AKI cultures in presence of bile (17.25 mM) were used (see methods for detail). The purified linearised plasmid <t>pBR322</t> (lin PstI) served as an extracellular DNA control. V. cholerae WT grown in AKI or SOC: HEPES served as recipient and was incubated for 20 h at 30°C and 150 rpm with respective BEVs VC1620/1:: tetR (2.5 × 10 11 particles) or pBR322 linearised with PstI (100 ng) before total CFU and transformants were determined by plating on LB‐Sm and LB‐Tet plates. (b) HGT rate of the tetR ‐cassette using BEVs VC1620/1:: tetR derived from AKI cultures in presence of bile (17.25 mM) as donor and V. cholerae WT or diverse V. cholerae mutants as recipient. Recipients, grown in AKI or SOC: HEPES, were incubated for 20 h at 30°C and 150 rpm with respective BEVs VC1620/1:: tetR (2.5 × 10 11 particles) before total CFU and transformants were determined by plating on LB‐Sm and LB‐Tet plates. (c) Internalisation of BEVs VC1620/1:: tetR by V. cholerae WT. Rhodamine‐labelled BEVs VC1620/1:: tetR derived from AKI cultures in presence of bile (17.25 mM) or MMC (60 ng mL −1 ) or without any stressor (control, co) were incubated with V. cholerae WT for 20 h. In addition, trypsin digested BEVs VC1620/1:: tetR derived from AKI cultures in presence of bile (17.25 mM) were used (see methods for detail). (d) Internalisation of BEVs VC1620/1:: tetR derived from AKI cultures in presence of bile (17.25 mM) by V. cholerae WT or the deletion mutants ∆ pilA and ∆ comEA . (c, d) Uptake is detected by an increase in relative fluorescence units (RFU) measured every 30 min. Wells containing rhodamine‐labelled BEVs VC1620/1:: tetR without recipient cells served as a blank. Shown is the median ± IQR ( n = 13). The bar chart on the right shows the median area under the curve (AUC) ± IQR calculated from the internalisation assays. Asterisks highlight significant differences between respective data sets (* p < 0.05 Kruskal–Wallis test followed by post hoc Dunn's multiple comparisons). (e) Colonisation fitness of VC1620/1:: tetR and ∆ comEA . Results are shown as competitive index (CI) for competitions of VC1620/1:: tetR or ∆ comEA to a fully virulent LacZ − derivative of the WT ( lacZ − ) in LB (in vitro) and in adult mice (in vivo). Each circle represents the CI from a single assay. Horizontal bars and error bars indicate the median ± IQR. Asterisks highlight data sets with significantly different CI from the theoretical value of 1 [ p < 0.05, Wilcoxon test against a hypothetical value of 1, n = 14 for VC1620/21:: tetR /WT ( lacZ − ) in vitro; n = 12 for ∆ comEA /WT ( lacZ − ) in vitro; n = 8 for VC1620/21:: tetR /WT ( lacZ − ) in vivo; and n = 6 for ∆ comEA /WT ( lacZ − ) in vivo]. (f) In vivo HGT rate of the tetR ‐cassette using BEVs VC1620/1:: tetR derived from AKI cultures in presence of bile (17.25 mM) as donor and V. cholerae WT or ∆ comEA as recipient. Mice colonised with WT or ∆ comEA received BEVs VC1620/1:: tetR VC1620/1:: tetR by oral gavage twice a day for two days before mice were euthanised and total CFU and transformants were determined by plating the homogenised colon on LB‐Sm and LB‐Tet plates. (a, b, f) Data are presented as median ± IQR. Assays yielding in no transformants on LB‐Tet were set to limit of detection (LOD), which is indicated by a dotted line. The LOD was defined as 0.5 CFU detected in the highest concentration plated on LB‐Tet plates divided by the total CFU determined by plating on LB‐Sm plates. HGT rates significantly higher than the LOD were by evaluated by the Wilcoxon Signed Rank test against the hypothetical value of the LOD (* p < 0.05, n = 8 for Panels a and b, n = 10 for Panel f). Statistically significant differences between the bile‐induced BEVs VC1620/1:: tetR before and after trypsin digest ( n = 8) or between the WT and ∆ comEA colonised mice ( n = 10) were analysed via the Mann–Whitney U test (* p < 0.05). BEV, bacterial extracellular vesicle; IQR, interquartile range; MMC, mitomycin C.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7587/pmc13157587/pmc13157587__JEV2-15-e70301-g006.jpg)