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Image Search Results
Journal: Indian Journal of Hematology & Blood Transfusion
Article Title: RHD Genotyping by Molecular Analysis of Hybrid Rhesus box in RhD-Negative Blood Donors from Iran
doi: 10.1007/s12288-018-0992-3
Figure Lengend Snippet: PCR-RFLP analysis for RHD gene deletion. a Banding patterns of RHD−/RHD− homozygotes. There are 3 Pst I sites in the hybrid Rhesus box amplicons. Digestion with this enzyme ( Pst I) results in 179, 397, 564, 1887-bp fragments (lanes 1–9). b PCR-RFLP for RHD+/RHD− heterozygous samples. An additional 744-bp fragment results from downstream Rhesus box digestion and indicates the heterozygous situation (lanes 1–2). M: Molecular marker 100 bp (YT8503, YEKTA TAJHIZ AZMA, IRAN)
Article Snippet: The PCR products were digested with
Techniques: Marker
Journal: Translational Cancer Research
Article Title: Sini decoction-polysaccharide compound regulates proliferation, apoptosis, and glycolysis of liver cancer cells through PHLDA2/ANXA2
doi: 10.21037/tcr-24-1625
Figure Lengend Snippet: Identification of difference-related genes affected by SNDPC in HCC. (A) Heatmaps, (B) volcano plot, (C,D) GO, (E,F) pathway, and (G,H) Venn were used to analyze the difference-related genes in HCC. (I) Western blotting was used to determine the expression of PHLDA2 and SPINK1. The results are presented as the mean ± SD. ***, P<0.001. GO, Gene Ontology; TCGA, The Cancer Genome Atlas; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SNDPC, Sini decoction-polysaccharide compound; HCC, hepatocellular carcinoma; SD, standard deviation.
Article Snippet: After the membranes were incubated overnight, they were sealed with 5% bovine serum albumin for 2 h and with primary antibodies against Bcl-2 (ab196495; Abcam, Cambridge, UK), Bax (ab32503; Abcam), cleaved caspase 3 (25128-1-AP; Proteintech, Rosemont, IL, USA), caspase 3 (82202-1-RR; Proteintech), LDHA [#2012; Cell Signaling Technology (CST), Danvers, MA, USA], HK2 (22029-1-AP; Proteintech), PKM2 (#3198; CST), PHLDA2 (14661-1-AP; Proteintech),
Techniques: Western Blot, Expressing, Standard Deviation