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Structured Review

Affibody polypeptides
Polypeptides, supplied by Affibody, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polypeptide/affibody+ligands/us12653913-225-1-5
Average 86 stars, based on 1 article reviews
polypeptides - by Bioz Stars, 2026-11
86/100 stars

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Glycoproteomics:

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: As a result, the present inventors confirmed that the Affibody molecules with amino acid modifications (substitutions) at two positions in the full-length amino acid sequence of 58 amino acids caused no glycosylation and had enhanced binding ability to an antigen (especially, IL-6). .. Therefore, an aspect of the present invention is to provide a polypeptide (Affibody molecule) improved in protein purity and affinity for a target antigen through glycosylation prevention even when produced in eukaryotic cells. .. Another aspect of the present invention is to provide a polypeptide complex, which contains the polypeptide (Affibody molecule) and an antibody or an antigen-binding fragment thereof specifically binding to any target antigen and thus is improved in protein purity and affinity for a target antigen.

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: .. Features and advantages of the present invention are summarized as follows. (a) The present invention provides a polypeptide (Affibody molecule) improved in protein purity and affinity for IL-6 through the prevention of glycosylation even when produced in eukaryotic cells. (b) The present invention provides a polypeptide complex improved in protein purity and affinity for IL-6 by containing the polypeptide (Affibody molecule) and an antibody or an antigen-binding fragment thereof specifically binding to any target antigen. (c) The present invention provides respective methods for manufacturing the polypeptide (Affibody molecule) and the polypeptide complex composed of an antibody and antigen-binding fragment thereof. ..

Produced:

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: As a result, the present inventors confirmed that the Affibody molecules with amino acid modifications (substitutions) at two positions in the full-length amino acid sequence of 58 amino acids caused no glycosylation and had enhanced binding ability to an antigen (especially, IL-6). .. Therefore, an aspect of the present invention is to provide a polypeptide (Affibody molecule) improved in protein purity and affinity for a target antigen through glycosylation prevention even when produced in eukaryotic cells. .. Another aspect of the present invention is to provide a polypeptide complex, which contains the polypeptide (Affibody molecule) and an antibody or an antigen-binding fragment thereof specifically binding to any target antigen and thus is improved in protein purity and affinity for a target antigen.

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: .. Features and advantages of the present invention are summarized as follows. (a) The present invention provides a polypeptide (Affibody molecule) improved in protein purity and affinity for IL-6 through the prevention of glycosylation even when produced in eukaryotic cells. (b) The present invention provides a polypeptide complex improved in protein purity and affinity for IL-6 by containing the polypeptide (Affibody molecule) and an antibody or an antigen-binding fragment thereof specifically binding to any target antigen. (c) The present invention provides respective methods for manufacturing the polypeptide (Affibody molecule) and the polypeptide complex composed of an antibody and antigen-binding fragment thereof. ..

Binding Assay:

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: Therefore, an aspect of the present invention is to provide a polypeptide (Affibody molecule) improved in protein purity and affinity for a target antigen through glycosylation prevention even when produced in eukaryotic cells. .. Another aspect of the present invention is to provide a polypeptide complex, which contains the polypeptide (Affibody molecule) and an antibody or an antigen-binding fragment thereof specifically binding to any target antigen and thus is improved in protein purity and affinity for a target antigen. .. Still another aspect of the present invention is to provide a method for producing the polypeptide (Affibody molecule) and a method for producing the polypeptide complex containing the polypeptide and the antibody or the antigen-binding fragment thereof.

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: .. Features and advantages of the present invention are summarized as follows. (a) The present invention provides a polypeptide (Affibody molecule) improved in protein purity and affinity for IL-6 through the prevention of glycosylation even when produced in eukaryotic cells. (b) The present invention provides a polypeptide complex improved in protein purity and affinity for IL-6 by containing the polypeptide (Affibody molecule) and an antibody or an antigen-binding fragment thereof specifically binding to any target antigen. (c) The present invention provides respective methods for manufacturing the polypeptide (Affibody molecule) and the polypeptide complex composed of an antibody and antigen-binding fragment thereof. ..

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: .. As used to indicate an aspect of the present invention, the term “complex” is used to designate two or more linked polypeptide chains, of which one component is a polypeptide (Affibody molecule) having affinity for a target antigen (e.g., IL-6) as defined above and the other component is an antibody or an antigen-binding fragment thereof specifically binding the target antigen. ..

Conjugation Assay:

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: .. Therefore, the polypeptide (Affibody molecule) and the antibody or the antigen-binding fragment thereof may be linked by means of chemical conjugation (using known organic chemistry methods) or by any other means (for example, via the expression of the complex as a fusion protein, or either directly, or indirectly via a linker (e.g., an amino acid linker)). ..

Expressing:

Article Title: Polypeptide improved in protein purity and affinity for antigen, conjugate thereof with antibody or antigen-binding fragment, and preparation method therefor
Article Snippet: .. Therefore, the polypeptide (Affibody molecule) and the antibody or the antigen-binding fragment thereof may be linked by means of chemical conjugation (using known organic chemistry methods) or by any other means (for example, via the expression of the complex as a fusion protein, or either directly, or indirectly via a linker (e.g., an amino acid linker)). ..



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